Mechanism of "Szechwan Chinaberry Fruit-Rhizoma Corydalis" drug combination in treatment of liver cancer based on network pharmacology / 临床肝胆病杂志

Objective To investigate the pharmacological components of "Szechwan Chinaberry Fruit-Rhizoma Corydalis" drug combination and its potential molecular mechanism in the treatment of liver cancer based on network pharmacology. Methods Related databases, such as TCMSP, Uniprot, and GeneCard, were used to obtain the effective components of Szechwan Chinaberry Fruit and Rhizoma Corydalis, their corresponding action targets, and the disease targets of liver cancer, and the intersecting targets of drugs and diseases were selected. In addition, STRING and Metascape databases were used to screen out the core targets of drug action and perform GO function and KEGG pathway enrichment analyses. Results There were 9 active components in Szechwan Chinaberry Fruit and 49 active components in Rhizoma Corydalis, with 1 common component between the two drugs; there were 181 action targets of Szechwan Chinaberry Fruit and 1097 action targets of Rhizoma Corydalis, with 143 common targets between the two drugs. There were 162 intersecting targets between the drug combination and liver cancer, and the main genes involved were IL6, TP53, VEGFA, TNF, and CASP3. KEGG analysis showed that the main pathways involved included cancer pathway, AGE-RAGE signaling pathway of diabetes complications, TNF signaling pathway, NF-κB signaling pathway, and thyroid hormone signaling pathway. Conclusion There are many different components in the drug combination of "Szechwan Chinaberry Fruit-Rhizoma Corydalis", which can exert a therapeutic effect on liver cancer by acting on related genes and signaling pathways.

GC-MS in analysis of chemical constituents of volatile oils in drug pair of Radix Angelica dahurica and Ligusticum chuanxiong Hort / 第二军医大学学报

Objective To study the essential oil components in herbal pair (HP) of Radix Angelica dahurica-Ligusticum chuanxiong Hort. (RAD-LCH) and its single herb, and to investigate the influence of HP on the components of the volatile oil. Methods Essential oil was extracted from Radix Angelica dahurica and Ligusticum chuanxiong Hort. by steam distillation. Identification of extracted componentswas accomplished using GC-MS technique combined with library searching and literature comparison. Chromatography analysiswas carried out on a TR5-MS capillary column (30 m × 0. 25 mm, 0. 25 μm); the carrier gas was high-purity helium; the inlet temperature was 250°C; the injection volume was 1 μL, the split ratio 50: 1; constant current mode, the flow rate of carrier gas was 1. 00 mL/min; the temperature program was as follows: 50C for one minute, rose to 240C at 5°C/min and maintained for one minute, then rose to 280C at 5°C/min and maintained for one minute. MS condition was as follows: electron impact (El) ion source, electron energy 70 eV, ion source temperature 200°C, transmission line temperature 280°C, mass scan range m/z: 50-650 amu, data acquisition scan mode for full scan, and solvent delay five minutes. Results There were 69, 59, 59, 60 and 78 volatile constituents in RAD, LCH, HP (1:1), HP (4: 1) and essential oil of mixture of equal concentration from RAD and LCH (EOEC), and 19 common volatilecomponents were identified. Conclusion Regarding the volatile oil constituents and content, the volatile oil compositions of different HP proportions vary greatly, accompanied by changed contents of volatile oil components of single herb in HP.

Review on Research of Compatibility Mechanism of Chemical Ingredients in Prescriptions with Radix et Rhizoma Rhei / 世界科学技术-中医药现代化

Radix et Rhizoma Rhei, the major usage of which is purgation, is widely used in clinical therapy among different prescriptions. According to present reports, researches on herbal compatibility mechanism were mainly fo-cused on the comparison of content changes of active ingredients before and after. In oder to provide references, this review was made on researches of compatibility mechanism of prescriptions and paired drugs for ingredients of Radix et Rhizoma Rhei from research actuality.

The Medicine Experience of Commonly Used Paired Drugs and The Three Drugs Pairing of Mr. Chen Zugao / 浙江中医药大学学报

Objective]Aimed to inherit Mr Chen Zugao's clinical experience and understand his medical characteristics. [Methods]Through the review of the herbal medicine books, summarized the experience of commonly used drugs(paired drugs and the three drugs pairing) of Mr. Chen. [Results] Completed the summary about Mr Chen's 8 groups of paired drugs(dui yao) and the three drugs pairing(jiaoyao): guizhi, digupi; guizhi, gansui;chuanshanjia, zaojiaoci; baijiezi, jiuxiangchong; huangqi, tianhuafen; qinjiu, baijili; fuzi, xixin, shigao; huangqi, zhimu, taizishen. [Conclusions]The paired drugs(dui yao) and the three drugs pairing(jiao yao) are the main medicine prescription drugs. A lot of applications wil be done in order to achieve good results. Inferiors wil be harvested who can have careful study of Mr. Chen's experience.