Enfermedad del desgaste en praderas de Thalassia testudinum (Hydrocharitaceae) y su relación con el perfil metabólico / Wasting disease in Thalassia testudinum (Hydrocharitaceae) meadows and its relationship with metabolic profile

Resumen Introducción: Los protistas del género Labyrinthula causan la denominada "Enfermedad del desgaste" en el pasto marino, Thalassia testudinum. Desde el 2008 los monitoreos en el Caribe colombiano han mostrado variación espacial y temporal en la incidencia de la enfermedad, pero sin la alta mortalidad observada en otras regiones del mundo. Objetivo: Analizar algunos parámetros epidemiológicos en T. testudinum y comparar metabolitos entre plantas sanas e infectadas. Métodos: Registramos la severidad, incidencia y prevalencia de esta enfermedad en el Parque Nacional Natural Tayrona e Isla de Providencia, y analizamos muestras de agua y sedimentos. Además, aplicamos cromatografía líquida y de gases, junto con espectrometría de masas, a extractos metanólicos de muestras de hojas y rizomas de brotes sanos e infectados. Resultados: Las praderas se encontraban en buen estado, a pesar de la escasez de brotes de fanerógamas marinas en Tayrona y una alta incidencia (15 %) y severidad (355 %) de la enfermedad en Providencia. Las plantas infectadas tenían niveles más bajos de fenoles, flavonoides y azúcares. Las flavonas sulfatadas con aglicona luteolina y diosmetina, los esteroles (sitosterol y estigmasterol) y las oxilipinas volátiles se acumularon en las hojas (3-hidroxi-2-isopentanona) y los ácidos isopentanoico y octadecatrienoico en los rizomas. Conclusiones: Estos pastos marinos colombianos tienen producción diferencial de metabolitos. Probablemente como una defensa exitosa, aún a niveles bajos de severidad (0.1 %) e incidencia (1 %) de la enfermedad.

Abstract Introduction: Protists of the genus Labyrinthula cause the so-called "Wasting Disease" in seagrass, Thalassia testudinum. Monitoring in the Colombian Caribbean since 2008 has shown spatial and temporal variation in the disease's incidence, but without the high mortality observed in other regions of the world. Objective: To analyze some epidemiological parameters in T. testudinum and to compare metabolites between healthy and infected plants. Methods: We recorded severity, incidence and prevalence of this disease in Tayrona National Natural Park and Providencia Island, and we analyze water and sediment samples. Additionally, we applied gas and liquid chromatography, coupled with mass spectrometry, to methanolic extracts from leaf and rhizome samples of healthy and infected shoots. Results: The meadows were in good condition, despite the scarce seagrass shoots in Tayrona and a high incidence (15 %) and severity (35.5 %) of the disease in Providencia. Infected plants had lower levels of phenols, flavonoids and sugars. Sulphated flavones with aglycone luteolin and diosmetin, sterols (sitosterol and stigmasterol) and volatile oxylipins are accumulated in leaves (3-hydroxy-2-isopentanone) and isopentaenoic and octadecatrienoic acids in rhizomes. Conclusions: These Colombian seagrasses have differential production of metabolites. Probably as a successful defense, even at low levels of severity (0.1 %) and incidence (1 %) of the disease.

Tomato (Solanum lycopersicum L.) and Fusarium oxysporum f. sp. lycopersici interaction. A review / Interacción entre tomate (Solanum lycopersicum L.) y Fusarium oxysporum f. sp. Lycopersici. Una revisión

ABSTRACT The interaction between plants and pathogens is a very dynamic and complex relationship that also includes a high degree of specificity, and it is precisely this last characteristic which triggers such important responses in the survival of one or the other. The pathosystem formed by tomato (Solanum lycopersicum L.) and Fusarium oxysporum f. sp. lycopersici (Fol) has been the subject of multiple studies due to the importance of the vegetable worldwide and for the economic and ecological impact of the fungus responsible for the vascular wilt disease in tomato, causing losses that go up to 100%. One way to find alternatives for the management of any pathosystem is to know the actors involved and the mechanisms that govern the interaction through technological and scientific advances that clearly show how the interaction develops on a genetic level. This review collects the information from different scientific sources with focus on the knowledge of the fungus, tomato cultivation and plant defense applied to this pathosystem, as well as the molecular mechanisms.

RESUMEN La interacción entre plantas y patógenos es una relación muy dinámica y compleja, que conlleva un alto grado de especificidad y es esta última característica, la que desencadena respuestas tan importantes en la supervivencia de uno u otro. El patosistema formado por tomate (Solanum lycopersicum L.) y Fusarium oxysporum f. sp. lycopersici (Fol) ha sido objeto de múltiples estudios, debido a la importancia de la hortaliza, a nivel mundial y por el impacto económico y ecológico del hongo, responsable de la marchitez vascular, provocando pérdidas que llegan hasta el 100%. Una forma de encontrar alternativas para el manejo de cualquier patosistema es conocer los actores involucrados y los mecanismos que rigen la interacción, a través de avances tecnológicos y científicos, que muestren, claramente, cómo se desarrolla la interacción, a nivel genético. Esta revisión recoge la información de fuentes científicas con énfasis en el conocimiento del hongo, el cultivo del tomate y la defensa vegetal, aplicada a este patosistema, así como los mecanismos moleculares.

Critical yield-point model to estimate damage caused by brown spot and powdery mildew in barley / Modelo do ponto crítico para estimar danos causados pela mancha-marrom e oídio em cevada

Barley (Hordeum vulgaris L.) is the second most important winter crop in Southern Brazil. The excessive rainfall in this region during the crop-growing season increases the frequency and intensity of foliar fungal diseases. The research aimed to determine the damage function equations (DFE) for the multiple pathosystem of barley brown spot and powdery mildew based on the relationship between grain yield and diseases intensity at different 'BRS Cauê' cultivar growth stages (GS) during 2009 and 2010 growing seasons in Southern Brazil. The experiments were arranged in a randomized complete block design with nine treatments and four replicates. The disease gradients were generated by strobilurins and triazols fungicides rates and number of applications on barley cv. Cauê. The fungicide applications and disease incidence and severity assessments were performed at the 22, 31, 39, 45 and 56 plant GS. The DFE were obtained by variance analysis and linear regression between grain yield and diseases intensity. Significant and negative DFE were obtained and the damage coefficients (DC) varied from 29.48 to 100.08 (2009) and from 36.08 to 113.57kg ha-1 (2010) for incidence, and from 219.5 to 6,276.6 (2009) and 102.3 to 5,292.5kg ha-1 (2010) for severity. The largest damage coefficients were obtained when diseases assessments were made on GS 22 and 31 on both growing seasons evaluated. DFE were used to calculate the economic damage threshold (EDT) as a criterion to indicate the fungicide application moment to control the diseases in cultivars similar to 'BRS Cauê' in Southern Brazil.

A cevada (Hordeum vulgaris L.) é a segunda mais importante cultura de inverno no Sul do Brasil. Nessa região, o excesso de chuvas durante a estação de crescimento da cultura favorece o aumento na frequência e intensidade de doenças foliares. O trabalho objetivou determinar as equações de função de dano (EFD) para o patossistema múltiplo mancha-marrom e oídio da cevada pela relação entre rendimento de grãos e intensidade foliar das doenças em diferentes estádios fenológicos da cultivar de cevada 'BRS Cauê', nas safras 2009 e 2010 no sul do Brasil. O delineamento foi em blocos casualizados com nove tratamentos e quatro repetições. Os tratamentos foram constituídos de diferentes doses e número de aplicações dos fungicidas triazóis e estrobilurinas para gerar os gradientes de intensidade das doenças. As aplicações de fungicidas e as avaliações da incidência e severidade foliar foram realizadas nos estádios fenológicos (EF) 22, 31, 39, 45 e 56. As equações foram obtidas pela análise de variância e regressão linear entre rendimento de grãos e intensidade das doenças. As EFD foram significativas e negativas e os coeficientes de danos variaram de 29,48 a 100,08kg ha-1 (2009) e 36,08 a 113,57kg ha-1 ( 2010) para a incidência e de 219,5 a 6.276,6kg ha-1 ( 2009) e 102,3 a 5.292,5kg ha-1 (2010). Os maiores coeficientes de dano foram obtidos nos estádios EF 22 e 31em ambas as safras. As EFD foram usadas para calcular o limiar de dano econômico, que é um critério que indica o correto momento de aplicação de fungicidas para o controle das doenças em cultivares similares a 'BRS Cauê' no sul do Brasil.