Penicillum marneffi infection presenting as lump abdomen.

Penicillum Marneffi is the third leading opportunistic pathogen in HIV endemic areas. We report a case, with a rare clinical presentation as lump abdomen with newly diagnosed HIV -1 antibody reactive status. A diagnosis was made by endoscopic biopsy of growth in second part of duodenum which on histopathology and culture detected Penicillum Marneffi. He was treated with intravenous amphotericin B for 2 weeks followed by oral itraconazole. This case is reported for its unusual presentation to sensitize clinicians to consider this as an etiology in patients in HIV endemic areas who present as lump abdomen.

Comparison of six methods for fungal genomic DNA extraction / 中华临床感染病杂志

Objective To identify a rapid and efficient fungal genomic DNA extraction method for PCR amplification.Methods Genomic DNA was extracted from Penicillum marneffei,Rhizopus microsporus,Cryptococcus neoformans and Candida albicans by heating pyrolysis,microwave,repeated freezing and thawing,lysozyme digestion,overnight snail enzymatic and Qiagen kit methods.DNA electrophoretogram was observed by gel imaging system.The concentration and purity of extracted DNA were determined with an ultramicro nucleic acid protein tester and the yields were calculated.PCR amplification and sequencing were also performed.ANOVA and SNK-q test were used for data analysis.Results There were statistical differences in concentrations and yields of the fungal DNA extracted from Penicillum marneffei (hyphal phase and yeast phase),Rhizopus microsporus,Coptococcus neoformans and Candida albicans by six methods (F=750.83,220.95,669.35,132.01,510.20 and 1658.35,287.10,963.64,1147.77,4521.22,all P ＜0.01).Of six methods,microwave method gained the highest DNA concentration and yield,followed by heating pyrolysis method,while Qiagen kit method obtained the lowest concentration and yield.All DNA extracted by 6 kinds of methods were positive in PCR amplification.Conclusion All of the six methods can be used for fungal DNA extraction which is sufficient for PCR amplification,but microwave and heating pyrolysis methods are more easy and simple to perform.