THE COMPARATION OF EXPERIMENTAL CONDITIONS EXTRACTING THE PROTEOLYTIC ENZYME FROM THE ALIMENTARY CANAL OF PNEUMATOPHORUS JAPONICUS / 中国海洋药物

Various conditions were compared for extracting the proteolytic enxyme from the alimentary canal of Pneumatophorus juponicus (Houttuyn). The results showed that when using CaCl2 as activator, the suitable conditions were pH7. 5,extracting temperature 20 C,extracting time 2h,activiting time 16h,70% alcohol precipitaion.