RESUMO
ObjectiveTo investigate the mechanism of dexamethasone (Dex) in inhibiting monocyte adhesion and phagocytose function.Methods Under the stimulation of phorbo1-12-myristate-13-acetate (PMA),U937 monocytes cultured in vitro were treated with Dex and Fasudil respectively.The adhesion rate of U937 monocles to human umbilical vein endothelial cells (HUVECs) and their phagocytic ability of India ink were studied.The protein content and activity of rho-associated coiled-coil protein kinase 1 ( ROCK1 ) as well as the effects of mifepristone and cycloheximide on Dex were determined.ResultsBoth DEX and Fasudil could significantly inhibit the adhesion tate and phagocytosis of U937 cells stimulated by PMA and suppressed the activity of ROCK1.While mifepristone and cycloheximide could not alter these effects of DEX.ConclusionDEX interferes with the adhesion and phagocytosis function of U937 cells by inhibiting ROCKI activity.
RESUMO
Codnopsispilosula polysaccharides (CPP) , which administered intra-peritoneally 200mg/kg?d-1 for 5 d or 8 d in mice, enhanced thephagocytic activity of peritoneal macrophages on chiken RBC, the erythrocyte (E)rosette formation of thymus T lymphocyte and the clearance rate of charcoal particles in normal mice and antagonized the inhibition of E rosette formation and of peritoneal phagocytic activity caused by cyclophosphamide ( CY ) or hydrocortisone ( HCT ) . It inhibited the delayed type hypersensitivity ( DT H ) induced by DNCB and enhanced DT H induced by dexamethasons ( DMS ) . It has regulatory important signification in resisting decrepitude.