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1.
Braz. j. med. biol. res ; 40(1): 19-25, Jan. 2007. ilus, tab
Artigo em Inglês | LILACS | ID: lil-439673

RESUMO

Lithium has been used for the last five decades to treat bipolar disorder, but the molecular basis of its therapeutic effect is unknown. Phosphoglucomutase is a key enzyme in the metabolism of glycogen. In yeast, rabbit and human HEK293 cells, it is inhibited by lithium in the therapeutic concentration range. We measured the phosphoglucomutase activity in erythrocytes and the inhibitor constant for lithium in a population of healthy subjects and compared them to those of bipolar patients treated with lithium or carbamazepine. The specific activity of phosphoglucomutase measured in vitro in erythrocytes from control subjects presented a normal distribution, with the difference between the lowest and the highest activity being approximately 2-fold (0.53-1.10 nmol mg Hb-1 min-1). Comparison of phosphoglucomutase activity in untreated bipolar patients and control subjects showed no significant difference, whereas comparison between bipolar patients treated with carbamazepine or lithium revealed significantly lower mean values in patients treated with carbamazepine (747.3 ± 27.6 vs 879.5 ± 35.9 pmol mg Hb-1 min-1, respectively). When we studied the concentration of lithium needed to inhibit phosphoglucomutase activity by 50 percent, a bimodal distribution among the population tested was obtained. The concentration of LiCl needed to inhibit phosphoglucomutase activity by 50 percent was 0.35 to 1.8 mM in one group of subjects and in the other it was 3 to 4 mM. These results suggest that phosphoglucomutase activity may be significant in patients with bipolar disorder treated with lithium and carbamazepine.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Antimaníacos/uso terapêutico , Transtorno Bipolar/tratamento farmacológico , Carbamazepina/uso terapêutico , Eritrócitos/enzimologia , Lítio/uso terapêutico , Fosfoglucomutase/efeitos dos fármacos , Antimaníacos/farmacologia , Escalas de Graduação Psiquiátrica Breve , Transtorno Bipolar/enzimologia , Estudos de Casos e Controles , Carbamazepina/farmacologia , Lítio/farmacologia , Fosfoglucomutase/metabolismo
2.
Iatreia ; 13(1): 6-15, mar. 2000. tab
Artigo em Espanhol | LILACS | ID: lil-422924

RESUMO

Este estudio descriptivo de las enzimas Fosfoglucomutasa 1 (FGM 1), Esterasa D (EsD) y Fosfatasa ácida eritrocitaria (FAE) como marcadores genéticos polimórficos, se realizó en 145 muestras de sangre, tomadas a igual número de donantes de los tres principales bancos de sangre de la ciudad, por el método de electroforesis convencional, con sistema de refrigeración, en gel de agarosa.La probabilidad de identificación para cada uno de los marcadores fue buena; la mejor fue la subtipificación de la FGM1, seguida de la FAE por ser las más polimórficas. Igual resultado se obtuvo para el poder de discriminación.Las frecuencias alélicas y fenotípicas se encontraron de manera representativa en la población, en la que estaban presentes 21 de los 22 fenotipos posibles.Los resultados obtenidos muestran que los tres marcadores enzimáticos estudiados se encuentran en equilibrio de Hardy-Weinberg, lo cual se comprobó aplicando el método estadístico ?2 y la corrección de Bonferroni para la FGM 1 subtipo; por ello son de gran utilidad para cálculos de probabilidad en casos forenses con población residente en Antioquia, pues se encontró que dada la procedencia de los donantes y sus padres se puede hacer inferencia a todo el departamento.


Assuntos
Fosfoglucomutase , Esterases , Isoenzimas , Fosfatase Ácida , Marcadores Genéticos
3.
Journal of Kunming Medical University ; (12)1986.
Artigo em Chinês | WPRIM | ID: wpr-516269

RESUMO

A method of typing of EsD-PCM_1-CLOI on the same gel electrophoresis has been devel- oped by improving and mixing the individual tests of typing EsD, PGM_1and GLOI. The method supplies a reliable, economical and practical means for more wide-spread use of EsD, PGM_1 and GLOI in the forensic medicine field.

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