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1.
Chinese Journal of Biotechnology ; (12): 4056-4065, 2021.
Artigo em Chinês | WPRIM | ID: wpr-921486

RESUMO

Photorhabdus is a Gram-negative bacterium from the family Enterobacteriaceae that lives in a symbiotic association with nematode or insects. In addition to the role of being insect pathogens, one species called Photorhabdus asymbiotica (Pa) causes human infection around the world. Nevertheless, how does this transkingdom infection occur remains elusive. Here we focus on one pathogenic determinant called Photorhabdus virulence cassette (PVC) that is founded in the Pa genome and many other pathogens. The RNA-seq and qPCR data showed that the NF-κB and MAPK pathways were drastically activated in the PVC-treated mammalian macrophages. Western blotting assays using samples treated with various inhibitors of the affected pathways confirmed the results we have observed for MAPK pathway previously. p65 translocation assays validated the NF-κB activation in the macrophages after PVC treatment. Moreover, the bacterial phagocytosis by macrophage was also promoted by PVC at the early stage, and this phagocytosis was inhibited by cytoskeleton inhibitors. Thus, the results indicated that PVC is involved in the bacterial invasion by activating NF-κB and MAPK signaling pathway, providing a new perspective for analyzing the pathogenicity of Pa in human infections.


Assuntos
Animais , Humanos , Macrófagos , NF-kappa B/genética , Photorhabdus , Transdução de Sinais , Virulência
2.
Asian Pacific Journal of Tropical Biomedicine ; (12): 31-36, 2018.
Artigo em Chinês | WPRIM | ID: wpr-950464

RESUMO

Objective: To evaluate the efficacy of symbiotic bacteria, Xenorhabdus indica, Xenorhabdus stockiae, Photorhabdus luminescens subsp. akhurstii and Photorhabdus luminescens subsp. hainanensis as a larvicide against Aedes aegypti and Aedes albopictus. Methods: Larvae (L3-L4) of Aedes aegypti and Aedes albopictus were given 2 mL of a suspension 10

3.
Asian Pacific Journal of Tropical Biomedicine ; (12): 31-36, 2018.
Artigo em Chinês | WPRIM | ID: wpr-700093

RESUMO

Objective:To evaluate the efficacy of symbiotic bacteria,Xenorhabdus indica,Xenorhabdus stockiae,Photorhabdus luminescens subsp,akhurstii and Photorhabdus luminescens subsp.hainanensis as a larvicide against Aedes aegypti and Aedes albopictus.Methods:Larvae (L3-L4) of Aedes aegyptiand Aedes albopictus were given 2 mL of a suspension 107-108 CFU/mL of each symbiotic bacterium.Distilled water and Escherichia coli ATCC· 25922 were used as the control.The morality rate of the larval mosquitoes was observed at 24,48,72 and 96 h.The experiment was performed in triplicates.Results:The larvae of both Aedes species started to die at 24 h exposure.Aedes aegypti showed the highest mortality rate (87%-99%),96 h after exposure to Xenorhabdus stockiae (bNBP22.2_TH).The mortality rate of Aedes albopictus was between 82% and 96% at 96 h after exposure to Xenorhabdus indica (bKK26.2_TH).Low effectiveness of distilled water and Escherichia coliATCC· 25922 were observed in both Aedes larvae,with a mortality rate of 2% to 12%.Conclusions:The study confirms the oral toxicity of Xenorhabdus and Photorhabdus bacteria against Aedes spp.Xenorhabdus stockiae and Xenorhabdusindica may be an alternative agent for control Aedes spp.This is basic information for further study on the mechanism of action on Aedes larvae or application to control mosquito larvae in the community.

4.
Microbiology ; (12)1992.
Artigo em Chinês | WPRIM | ID: wpr-685771

RESUMO

The symbiotic bacterium exists in the intestines of entomopathogenic nematodes and is a potential biological agent.Systematic classification of these bacteria is scarce in China.In this paper,seven strains of symbiotic bacteria from local entomopathogenic nematodes were identified by both observation of mor-phology,physiological,biochemical characteristics and sequence analysis of 16S rDNA fragments.

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