RESUMO
Eleven compounds were purified from the anti-inflammatory extract of Picris davurica by column chromatograph on HP20 macroporous resin, MCI, sephadex LH-20 and ODS.Their structures were determined by the spectra data of NMR and MS as isoestin 7-O-β-D-glucopyranosyl-2'-O-α-L-arabinopyranoside(1), isoetin-7-O-β-D-glucopyranosyl-2'-O-β-D-xyloypyranoside(2), isoetin-7-O-β-D-glucopyranosyl-2'-O-α-D-glucopy- ranoside(3), isoetin 5'-O-β-D-glucopyranoside(4), isoetin-2'-O-β-D-xyloypyranoside(5), isoetin-4'-O-β-D-O-β-D-glucopyranoside(6), isoetin-7-O-β-D-glucopyranoside(7), isoestin-2'-O-α-L-arabinopyranoside(8), luteolin-7-O-β-D-glucopyranosie(9), isoetin(10) and luteolin (11). All compounds were isolated from this plantfor the first time.Among them, compounds 1-8 were isolated from the genus Picris for the first time.
RESUMO
Objective: To study the chemical constituents from the aerial part of Picris davurica and their anti-oxidative activity. Methods: Compounds were isolated by column chromatography of MCI, Sephadex LH-20, and ODS; Their structures and anti-oxidative activity were elucidated by NMR, MS, and DPPH methods. Results: On the basis of 1D-NMR, 2D-NMR, and ESI-MS data, eleven compounds were identified as caffeic acid (1), caftaric acid (2), chlorogenic acid (3), chicoric acid (4), 5-O-caffeoylshikimic acid (5), 3,4-di-O-caffeoylquinic acid (6), 3,5-di-O-caffeoylquinic acid (7), 4,5-di-O-caffeoylquinic acid (8) 4-O-caffeoylquinic acid (9), 5-O-caffeoylquinic acid (10), and 3,4-dihydroxy-6-(3,4-dihydroxy-E-styryl)-2-pyron-3-O-β-D-glucopyranoside (11). Conclusion: All the compounds reported in this study are isolated from the aerial part of P. hieracioides for the first time and show certain anti-oxidative activity.