RESUMO
Objective: To study the chemical constituents of Pilea sinofasciata. Methods: Column chromatography, such as silica gel, Sephadex LH-20, and preparative HPLC, was used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR, and 13C-NMR, and the physical constants were used to elucidate their structures. Results: Nineteen compounds were isolated from 95% ethanol extracts of P. sinofasciata, including α-tocopherol (1), stigmasterol (2), epihernandulcin (3), hernandulcin (4), benzoic acid (5), ethyl linolenate (6), ethyl hexadecanoate (7), α-amyrin (8), palmitic acid (9), behenic acid (10), adenosine (11), indole-3-carboxylic acid (12), protocatechuate (13), gallic acid (14), betulinic acid (15), oleanolic acid (16), potassium nitrate (17), diosmetin 7-O-β-D-glucopyranoside (18), and 3-O-β-D-xylopyranosyl (1→2)-β-D-glucopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid (19). Conclusion: All of the compounds are isolated from the plant for the first time.
RESUMO
OBJECTIVE: To study the antibacterial constituents of Pilea notata C. H. Wright.