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@#【Objective】 To study the effects of soil environment on the growth, yield, and quality of Sharen (Amomi Fructus) under different planting patterns. 【Methods】 Soil physical and chemical indices and enzyme activities in four periods including early flowering (March), full flowering (June), fruit ripening (September), and late fruit picking (December), were measured under three planting patterns including natural forest, greenhouse, and rubber forest in Xishuangbanna, China. The changes in soil indices during the growth periods of Sharen (Amomi Fructus) under different planting patterns were analyzed, and the differences in plant growth, yield, and quality under different planting patterns were explored. Pearson correlation analysis was used to analyze the relationship between soil indices and Sharen (Amomi Fructus) growth, yield, and quality. Principal component analysis was used to investigate the effects of soil environment under different planting patterns on Sharen (Amomi Fructus) growth, yield, and quality. 【Results】 The soil moisture, available potassium content, and urease activity of the three planting patterns of Sharen (Amomi Fructus) increased initially and decreased afterwards throughout the year; pH and organic matter content showed little change in the whole year. Exchangeable manganese content and acid phosphatase activity gradually increased throughout the year. Hydrolyzed nitrogen content, exchangeable calcium content, available zinc content, protease activity, and sucrase activity decreased initially and increased afterwards throughout the year. Exchangeable magnesium content, available iron content, and catalase activity decreased annually. Total nitrogen content, total phosphorus content, and available phosphorus content fluctuated throughout the year. The total potassium content under natural forest and greenhouse planting decreased throughout the year, while the total potassium content under rubber forest showed an upward trend all year round. The organic matter content, total nitrogen content, total potassium content, available potassium content, available zinc content, urease activity, acid phosphatase activity, and catalase activity under greenhouse were significantly lower than those under natural and rubber forests (P < 0.05). Correlation analysis showed that plant growth, yield, and quality of Sharen (Amomi Fructus) were significantly correlated with soil organic matter, total nitrogen, hydrolyzed nitrogen, total phosphorus, available phosphorus, total potassium, available potassium, exchangeable manganese, exchangeable magnesium, exchangeable calcium, available zinc, urease, acid phosphatase, and invertase (P < 0.05). The results of the principal component analysis indicated that the soil environment of Sharen (Amomi Fructus) under natural forest was the best, followed by rubber forest and greenhouse. The order of its advantages and disadvantages is consistent with the growth index of Sharen (Amomi Fructus), but contrary to the yield of Sharen (Amomi Fructus), indicating that the soil environment directly affects the growth index and nutritional components of plants. 【Conclusion】 Different planting patterns of Sharen (Amomi Fructus) have different soil nutrient content, and the change rules in the growths period are not similar, with some differences. Soil indices have impacts on plant growth, yield, and quality of Sharen (Amomi Fructus). Soil ecological environment is positively correlated with the growth characteristics of Sharen (Amomi Fructus) plants, but has no direct correlation with yield and quality.
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Objective: To establish a HPLC fingerprint method for assessing the quality of Dendrobium huoshanense, in addition to determining concentrations of syringic acid, rutin, dendrophenol and naringenin in this crude drug. Methods: Agilent C18 column (250 mm × 4.6 mm, 5 μm) was utilized with the mobile phase comprising methanol-0.1% phosphoric acid with the flow rate of 1 mL/min in a gradient elution manner. The detection wavelength was set at 240 nm and the column temperature was 30 ℃. The resultant chromatograms were imported to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica (2012.1) to obtain retention time and peak area of samples. Similarity for 39 sets of samples were analyzed. The peak area data were processed by SPSS software for cluster analysis and the clustering effect was discussed. Results: The line relationship of this way was good (R > 0.999), with high precision regarding instrument used (RSD < 3.00%), the method showed good reproducibility (RSD < 3.00%), standard recovery was between 99.26% and 100.32% (RSD of 0.35%-1.67%). Different growth period and different planting patterns of D. huoshanense were distinct regarding the concentration of four compounds. Conclusion: The method is useful to evaluate and discriminate D. huoshanense at different growth period for the purpose of providing scientific reference on harvest, development and evaluation of D. huoshanense.
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To evaluate the effects of herbicide rate, cultivar, and spatial pattern on rice grain yield and weed suppression, a field experiment was conducted on a lowland rice field at the Rice Research Station of Tonekabon, north of Iran, in 2011. The experimental design was a split plot-factorial where the whole plot portion was a randomized complete block with three replicates. Main plots were pretilachlor rates (0, 0.5, 1, 1.5, 2 L ha-1 of pretilachlor). The subplots were factorial combinations of two crop spatial patterns (uniform and wide-row planting patterns, 20× 20 and 30 × 13 cm, respectively) and two traditional rice cultivars ('Hashemi' and 'Deylamani'). Regardless of cultivar and spatial pattern, rice grain and biological yields, panicle number per m2, leaf area index, and herbicide efficacy increased, while weed biomass decreased with increasing pretilachlor application rate. Rice grain and biological yields, panicle number per m2, leaf area index, and herbicide efficacy were significantly greater in uniform planting pattern than in wide-row planting pattern when averaged over cultivars and pretilachlor rates. Moreover, uniform planting pattern of rice plants suppressed weeds 39% better than wide-row planting pattern as averaged across pretilchlor rates and cultivars. 'Deylamani' produced grater grain and biological yields, panicle number per m2, leaf area index than 'Hashemi' as averaged across pretilachlor rates and spatial patterns. This experiment illustrated that uniform spatial pattern increased weed suppression, herbicide efficacy, and grain yield.
Para avaliar os efeitos da taxa de herbicida, cultivar e padrão espacial em arroz produção de grãos e supressão de plantas daninhas , um experimento de campo foi realizado em um campo de arroz irrigado na Estação de Pesquisa do Arroz de Tonekabon , norte do Irã, em 2011. O delineamento experimental foi uma fração de lote - fatorial, onde toda a parte de enredo foi em blocos casualizados com três repetições. As parcelas foram as taxas pretilaclor (0, 0.5, 1, 1.5 , 2 L ha-1 de pretilaclor) . As subparcelas foram combinações fatoriais de dois padrões de culturas espaciais (uniformes e padrões de plantio em toda a linha, 20 × 20 e 30 × 13 cm , respectivamente) e duas cultivares de arroz tradicionais (' Hashemi ' e ' Deylamani ') . Independentemente de cultivar e padrão espacial , grãos de arroz e os rendimentos biológicos , número de panículas por m2 , o índice de área foliar e eficácia do herbicida aumentou, enquanto a biomassa de plantas daninhas diminuiu com o aumento da taxa de aplicação pretilaclor . O grão de arroz e os rendimentos biológicos , número de panículas por m2 , o índice de área foliar e eficácia do herbicida foram significativamente maiores no padrão de plantio uniforme do que em toda a linha de plantio padrão quando calculados sobre cultivares e taxas pretilaclor . Além disso, o plantio uniforme padrão de plantas de arroz suprimida ervas daninhas 39% melhor do que em toda a linha de plantio padrão como média entre as taxas de pretilchlor e cultivares . ' Deylamani "produzido grão ralador e os rendimentos biológicos , número de panículas por m2 , índice de área foliar do que" Hashemi ", como média entre as taxas de pretilaclor e padrões espaciais. Este experimento mostrou que padrão espacial uniforme aumento supressão de plantas daninhas , eficácia do herbicida , e rendimento de grãos.