Comparative performance of the protocol of plasma rich in growth factors - universal 1 (PRGF-U1) for obtaining platelet rich plasma

Objective: to compare the platelet concentration obtained after application of the protocol of plasma rich in growth factors - universal 1 (PRGF-U1) and the protocol of Anitua and Andia (PRP-A) for obtaining platelet rich plasma. Material and Method: A descriptive, cross-sectional and comparative study was carried out with a simple random probabilistic sample consisting of 16 patients who attended the Periodontics service of the Unit of Second Specialization in Stomatology of the National University of Trujillo. Five blood samples were obtained from each patient, after applying a health questionnaire to rule out any disease or drug consumption, in order to obtain the baseline platelet concentration and that obtained after PRGF-U1 and PRP-A. To compare the platelet concentrations of the two protocols, Student’s t-test was used considering a significance level of p<0.05. RESULTS: The baseline platelet concentration was 371,250 +/- 68,203 platelets/ μL, for PRGF-U1 it was 747,875 +/- 121,645 platelets/μL and for PRP-A it was 595,000 +/- 129,202 platelets/ML. A statistically significant difference (p<0.001) was found between both protocols. Conclusion: The PRGF-U1 protocol yielded a higher platelet concentration compared to the Anitua and Andia protocol.

Role of Autologous Platelet Derived Growth Factor and Fibrin Rich Plasma in Management of Chronic Non-healing Ulcer–A Pilot Study.

Background and Objectives: Chronic non-healing ulcer (CNHU) develops due to infections, trauma or underlying any medical and surgical conditions. Ulcer that have failed to response all available mode of treatment for long duration are more likely to develop gangrene and infection prone to limb amputation. This is a major public health problem. None of the conventional treatments are anticipated to stimulate active wound healing. The objectives of this study is to test the efficacy of topically applied autologous platelet derived growth factors and fibrin rich plasma in active repair of chronic non healing ulcer. Methods: Patients having one or more ulcers who have been receiving conventional treatment for their at least for more than 6 months duration but showed no evidence of healing till date were included in this study. Total of 30 skin ulcers were registered, of which 15 patients did not return for follow up. Rest 15 patients were included as the study group. All ulcers were treated with autologous platelet derived growth factors and fibrin rich plasma enriched antibiotic ointment. We observed that 73.33% ulcers were complete healed & rest ulcers had signs of improvement. Results: The study group showed complete healing in 73.33% ulcers and average 80% improvement observed in each ulcer, after applying autologous platelet derived growth factors and fibrin rich plasma. Significant ulcer healing was observed in patients who were less than 40 years of age, had no history of addiction for any toxic substance, and had ulcer size less than 30 sq cm. Ulcer healing rate was also found to be higher in cases whose duration of ulcer was within one year and those who did not have any history of systemic diseases. Conclusion: This study clearly shows the efficacy of topically applied autologous platelet derived growth factors and fibrin rich plasma in management of chronic non-healing ulcer.

The effect of rotation constancy static magnetic field on the expression of BMP-2 and PDGFs during fracture healing / 中华物理医学与康复杂志

Objective To evaluate the effect rotation constancy static magnetic field(RCSMF) on the expression of bone morphogenetic protein-2 (BMP-2) and platelet-derived growth factors (PDGFs) in fracture healing, and elucidate the possible mechanism of RCSMF promoting fracture healing.Methods A total of 80 rats with femur closed fracture were randomly divided into a magnetic treatment group (M) and a control group (C).The M group was given RCSMF treatment 30 minutes per day, 6 times per week, using 0.4 T 6 Hz magnetic field, while the C group was not given any intervention.Femurs and calluses were subjected to BMP-2, PDGF-A and PDGF-B immunohistochemistry assay at 2, 3, 4 and 8 weeks after the treatment.The integrated optical density (IOD) of positive staining was calculated.Two-factor variance analysis was used to compare the main effect of time, treatment and that of their interaction.Results For both groups, the IOD of immunoreactivity of BMP-2 at 2, 3 and 4 weeks after the treatment was significantly higher than that 8 weeks after the treatment, and the value at four weeks after the intervention was also significantly higher than that at 2 weeks after it.There was a significant interaction effect of time × treatment of BMP-2 (F =3.17, P ＜ 0.05).Significant differences were observed in the IOD of positive staining PDGF-A at different time points for both groups : the value at 2 weeks after treatment was significantly higher than that at 3, 4 and 8 weeks after the treatment, and that at 3 and 4 weeks after treatment was also significantly higher than that at 8 weeks after the treatment.The IOD of positive staining PDGF-B of the M group (57.6 ± 2.1) was significantly higher than that of the C group (50.11 ± 2.22, P ＜ 0.05).There was significant difference in the IOD of positive staining PDGF-B (F =50.06 ,P ＜ 0.01) at different time points : the IOD of positive staining PDGF-B at 3 weeks after treatment was significantly higher than that at 2 and 8 weeks group after the treatment, and the value at 2 and 4 weeks after treatment was also significantly higher that at 8 weeks after the treatment.Conclusion RCSMF may elevate the expression BMP-2 and PDGF-B to promote fracture healing.

Platelet rich fibrin: A new hope for regeneration in aggressive periodontitis patients: Report of two cases.

The purpose of this study was to evaluate the effectiveness of platelet rich fibrin (PRF) in periodontal regeneration in generalized aggressive periodontitis (GAgP) patients. Two patients diagnosed with GAgP were selected for this study. Baseline clinical and radiographic examination was performed and patients were treated by surgical periodontal therapy along with PRF. Post-surgical re-evaluation was carried out at regular intervals to evaluate clinical and radiographic changes. Surgical periodontal therapy accompanying the placement of PRF in angular defects of GAgP patients showed decreased probing pocket depth, increased attachment level and radiographic bone fill when baseline and 9 month follow-up data was compared. Surgical reconstructive therapy with placement of PRF in angular defects of GAgP patients can be an effective approach to enhance the periodontal regeneration.

Aplicação clínico-cirúrgica do plasma rico em plaquetas: estudo revisional / Clinical and surgical applications of prp: revisional study

Este trabalho teve como objetivo realizar uma revisão bibliográfica sobre a efetividade do Plasma Rico em Plaquetas (PRP). como foco na área da odontologia. A metodologia aplicada para a construção dessa pesquisa foi através de bancos de dados eletrônicos (BIREME, LILACS, BBO, Portal CAPES, PUBMED) e livros relacionados ao assunto, onde foram analisados os métodos de obtenção do PRP, suas ações através dos fatores de crescimento e suas aplicações clínicas. O PRP é uma concentração autóloga de plaquetas humanas em um pequeno volume de plasma, e é gerado a partir de uma preparação 100% orgânica realizada antes do procedimento cirúrgico. A sua utilização é considerada uma tática para aumentar e acelerar os efeitos dos fatores de crescimento contido nas plaquetas, iniciadoras universais da cicatrização, mostrando bons resultados em regeneração tecidual, principalmente em enxertos ósseos. A literatura possui críticos que questionam cientificamente a confiabilidade desse recurso, porém a maioria dos pesquisadores apresentou resultados clínicos bastante satisfatórios de sua aplicação.

The aim of this work was a bibliographical revision about the effectiveness of the Platelet-rich Plasma (PRP), with focus on dentistry. The methodology applied for the construction of this research was through electronic data bases (BIREME, LILACS, BBO, Portal CAPES, PUBMED) and books related to the subject, where the methods of attainmento of PRP, its action by growth factors and its clinical applications was analyzed. The PRP is an autologous concentration of human platelets in a small volume of plasma, and is created from a 100% organic preparation before the surgical procedure. Its use is a tactic to increase and to speed up the effect of growth factors contained in platelets, universal begining of healing, showing good results in tecidual regeneration, mainly in bone grafts. The literature has critics who question the trustworthiness of this material, however, the majority of these researchers related satisfactory results of its application.

Platelet-derived Growth Factors and Tissue Repair / 中国康复理论与实践

@#Platelet-derived growth factors (PDGFs) are a member of family of glycoprotein dimers (Mr 27000～35000) that exert potent mitogenic and chemotactic activities toward cells of mesenchymal origin. The PDGFs possess a myriad of critical roles in embryonic development,cellular differentiation and response to tissue damage. It is one of the growth factors,which appear early during the process of wound healing. Especially,PDGFs can effectively promote the healing of some chronic refractory wounds,such as diabetes mellitus ulcer,chronic venous ulcer,bedsore,radioactivity ulcer,etc..

A comparison of bioresorbable membranes alone or in combination with platelet-derived growth factors and insulin-like growth factors on the periodontal healing of the dehiscence defects in dogs / 대한치주과학회지

The purpose of present study is to compare the effect of treatment using Guidor(R) as a barrier membrane in conjunction with platelet-derived growth factor and insulin like growth factors on experimental dehiscence defects. Following the resection of premolar crowns, roots were submerged. After 12 weeks of healing period, experimental dehiscence defects of 4mm in height and 4mm in width were surgically created on the mid-facial aspect of the lower premolar roots in each of 4 adult dogs. After root planning and demineralization of the root surface with citric acid, the control groups received 4% methylcellulose gel only, the test group I received 4% methylcellulose gel and were covered by Guidor(R) and the test group II were treated with PDGF and IGF and 4% methylcellulose gel with Guidor(R) coverage. Histological and histomorphometric analysis following 8 weeks of healing revealed the following results. 1. The new bone formation showed no statistically significant difference in all groups with 0.59+/-0.82mm(14.03+/-19.60%) for control, 0.70+/-0.39mm(16.30+/-9.01%) for group I, 0.87+/-0.76mm(18.74+/-16.03%) for group II. 2. The new cementum formation showed no statistically significant difference in all groups with 0.54+/-0.48mm(16.38+/-14.57%) for control, 0.95+/-0.38mm(23.43+/-9.30%) for group I, 1.01+/-0.75mm(22.10+/-16.11%) for gorup II. 3. The root resorption showed statistically significant differences betweenthe control group and all test groups(p<0.05) with 2.11+/-0.53mm(52.93+/-12.32%) for control, 0.63+/-0.27mm(15.32+/-7.05%) for group I, 0.89+/-0.33mm (19.26+/-7.11%) for group II. On the bases of these results, there were no statistically difference between treatment using resorbable membrane and resorbable membrane in conjunction with PDGF and IGF in the dehiscence defects, where it was difficult to maintain space. The use of membrane seemed to be more effective in the inhibition of root resorption.

Significance of the expressions of VEGF mRNA, bFGF mRNA,PDGF mRNA and their receptors in pancreatic carcinoma / 中国普通外科杂志

Objective To study the effect of intratumor angiogenesis and vascular growth factor(VEGF), basic fibroblase growth factor(bFGF), Platelet-derived growth factor (PDGF), and their receptors(flt-1 bFGFR.PDGFR) on the invasion, metaslasis of pancreatic carcimoma(PC) and the relationship between the expressions of three kinds of angiogenic factors, their receptors and microvessel count(MVC). Methods Tis- sue sections of 51 PC and 32 acute or chronic pancreatitis were examined by in situ hybridization for the expression of VEGF bFGF.PDGF, and by immunohistochemistry for the expression of the three kinds of angiogenic factor receptors and MVC. The correlation of the expressions and pathological characteristics of PC were also studied. Results The positive rate of VEGF mRNA,bFGF mRNA, PDGF mRNA and their receptors in PC were significantly higher than that in acute or chronic pancreatistis( P