To study the effect of 'Covishield' vaccination on pre-donation platelet counts of plateletpheresis donors

ABSTRACT Introduction: Approximately 55.52% of the Indian population had been fully vaccinated by Jan. 2022, since its first roll out on January 16, 2021. A few concerns were raised concerning the Covishield vaccination related to thrombotic thrombocytopenia. Apheresis-derived platelet concentrates are frequently required in a plethora of clinical situations and post-vaccination decrement of platelet counts might lead to increased deferral of the plateletpheresis donors. Objectives. The aim of the study was to discover the effect of the Covishield vaccination on deferral rates of plateletpheresis donors. Methods: Blood samples were collected from the potential platelet donors for the completion of the standard questionnaire for the complete blood count. The data collected were tabulated in the MS Excel spreadsheet and the biostatistical analysis was performed with the SPSS v23. A p-value of < 0.05 was taken as significant. We compared this data with age-and sex-matched controls. Results: The mean age of cases and controls was 29.69 ± 8.57 and 30.15 ± 7.11, respectively. There was a significant difference in platelet counts of cases (188496.35 ± 72065.66/cumm) and controls (269524.50 ± 53981.60/cumm). Furthermore, donors who received one dose had higher platelet counts of 248676.47 ± 80075.24/cumm than those who received both doses of vaccine (179970.83 ± 66773.73/cumm). The difference in deferral rates between the two groups was remarkable (34.7% vs. 0.9%, with the p-value < 0.001). Conclusion: Vaccination certainly increased the deferral rates of plateletpheresis donors due to low platelet counts. Average platelet counts were low in fully vaccinated individuals, however, the platelets returned to normal counts as the post-vaccination days progressed.

Effect of double dose plateletpheresis on target yield and donor platelet recovery

Abstract Introduction The demand for apheresis platelets has increased in the recent past and the shrinking donor pool has shifted the trend to collection of double-dose or higher yield of platelets. Objective The present study aimed to determine the effect of double-dose plateletpheresis on the target yield and donor platelet recovery. Methods The study was conducted on 100 healthy plateletpheresis donors, 50 of whom were in the study group, which underwent double-dose plateletpheresis (DDP), and 50 of whom were in the control group for single-donor plateletpheresis. Pre- and post-procedure samples of donors were subjected to a complete blood count. The DDP product was sampled for platelet yield and then split into two parts. Platelet yield, collection efficiency, collection rate, recruitment factor and donor platelet loss were calculated. Results The mean platelet yield in the SDP was 4.09 ± 1.15 × 1011 and in the DDP, 5.93 ± 1.04 × 1011. There was a significant correlation between the pre-donation platelet count and platelet yield. The total of platelets processed for the SDP were 5.42 ± 1.08 × 1011 and for the DDP, 7.94 ± 0.77 × 1011. The collection efficiency was 71.93 ± 25.14% in the SDP and 72.94 ± 16.28% in the DDP, while the collection rates were 0.78 × 1011 and 0.94 × 1011 per minute, respectively. The average recruitment factor observed was 0.98 in the SDP, while it was 0.99 in the DDP. The mean platelet loss observed in the SDP was 35.55 ± 8.53% and in the DDP, 37.76 ± 8.65%. Conclusion The double-dose plateletpheresis supplements the platelet inventory in developing countries where the apheresis donor pool is limited. It is prudent to ensure stringent donor selection criteria for donors donating high-yield platelet products, thus enhancing donor safety and retention.

Utility of microcytic anemia factor and low hemoglobin density in the diagnosis of iron deficiency in blood donors / 中华检验医学杂志

Objective:The study was planned to evaluate the clinical utility of microcytic anemia factor (MAF) and low hemoglobin density (LHD%) in the screening of iron deficiency in blood donors.Methods:A total of 400 blood donors, 200 male and 200 female, were randomly admitted to Fujian Blood Center from January1, 2022 to February 28, 2022 by the way of stratified sampling. According to the fourth edition of Diagnostic and therapeutic criteria for hematological diseases, the patients were divided into three groups: normal group (N=299), iron depletion group (ID, n=54) and iron deficient erythropoiesis group (IDE, n=47), Blood routine indexes including hemoglobin (HGB), mean corpuscular volume hemoglobin (MCV), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration (MCHC) and iron metabolism indexes including serum ferritin (SF), serum iron (SI), total iron binding capacity (TIBC), transferrin saturation (TS) and unsaturated iron binding capacity (UIBC) were measured, MAF and LHD% were calculated by formula.One-way ANOVA or Kruskal-Wallis H tests were used to analyze the differences among three groups. Spearman correlation analysis was used to analyze the correlation between MAF and LHD% and iron metabolism indexes.The Receiver Operating Characteristic (ROC) curve was used to evaluated the diagnostic value of MCH, MCHC, MAF and LHD% for iron deficiency in blood donors. Results:MAF in ID group which was 11.81±0.81 were higher than the IDE group which was 10.69±0.95 and lower than the healthy group which was 13.17±1.24, the total difference among the three groups was statistically significant ( F=110.784, P<0.001), the difference between two groups was statistically significant ( P<0.01); LHD% in ID group which was 2.61 (1.87, 3.91)% were lower than the IDE group which was5.60(2.99, 8.02)% and higher than the healthy group which was1.74 (1.22, 2.73)%, the total difference among the three groups was statistically significant ( H=62.166, P<0.001), the difference between two groups was statistically significant ( P<0.01).In 101 iron deficiency blood donors, Spearman correlation analysis showed that MAF was positively correlated with SF, SI and TS ( r=0.426, P<0.01; r=0.547, P<0.01; r=0.566, P<0.01);contrarily, LHD% was negatively correlated with SF, SI and TS ( r=-0.397, P<0.01; r=-0.400, P<0.01; r=-0.479, P<0.01).The areas under the ROC curve of MCH, MCHC, MAF and LHD% diagnostic ID were 0.745, 0.646, 0.819 and 0.646, respectively;the cut-off value of MAF was 12.56, with a sensitivity of 67.90% and a specificity of 83.30%.While the areas under the ROC curve of MCH, MCHC, MAF and LHD% diagnostic IDE were 0.901, 0.834, 0.941 and 0.834, respectively; the cut-off value of MAF was 11.73, with a sensitivity of 87.60% and a specificity of 87.20%. Conclusions:MAF performed a high diagnostic value of iron deficiency, especially IDE, and can be used as a marker in the diagnosis of iron deficiency in blood donors.

Application of blood/fluid warmer during plateletpheresis in winter and its nursing / 中国输血杂志

【Objective】 To study the safety, effectiveness and nursing of blood/fluid warmer during the process of plateletpheresis in winter. 【Methods】 The blood re-transfusion speed during plateletpheresis in winter and the time of blood passing through the blood return pipeline was counted. The vitro blood was heated with a blood/fluid warmer under different temperature settings, and the rising speed of blood temperature was recorded. The blood samples were tested for blood routine examination, free Hb and erythrocyte morphology at 0, 15 and 30 minutes. In the process of plateletpheresis in winter, the blood donors′ ear temperature and the skin temperature near the reinfusion needle in the observation group and the controls were measured, and the blood donors were observed for shivering, arm chills, pain or other discomfort. After the blood donation, the thermal comfort was evaluated. 【Results】 There was no difference in the results of routine blood test and plasma free Hb test of vitro blood after warming at 41℃, 42℃ and 43℃ for 0, 15 and 30 minutes (P>0.05), and no change in erythrocyte morphology was found. The skin temperature near the reinfusion needle (before vs. after the start of phlebotomy) was statistically different by applying blood/fluid warmer or not(P0.05). The vitro blood heating experiment showed that when the room temperature was within 22~24℃, the blood retransfusion speed was (100-120) mL/min; after the application of blood/fluid warmer, the temperature of reinfusion blood could be raised from 27℃ to 33~37℃. The proportion of feeling comfortable and very comfortable and the score of thermal comfort in the blood donors who used the warmer were higher than those in the controls (P<0.05). When the temperature of the warmer was set above 38℃, the average score of thermal comfort of blood donors was above 8. 【Conclusion】 It is safe to apply the blood/fluid warmer during the plateletsapheresis in winter, which can significantly improve the comfort of blood donors and reflect the humanized service of blood stations, and is worth popularizing.

Research progress on iron nutritional status of plateletpheresis donors / 中国输血杂志

Apheresis platelets are extensively utilized in clinical practice due to high purity and minimal side effects. These platelets are primarily obtained from regular blood donors. However, there is no consensus on whether plateletpheresis leads to iron deficiency among blood donors. In recent years, increasing attention has been given to the impact of plateletpheresis on the iron nutritional status of these donors. Numerous studies have indicated a prevalence of iron deficiency among plateletpheresis donors. The process of plateletpheresis involves the loss of red blood cells, which can accumulate over time and disrupt iron metabolism, ultimately resulting in iron deficiency anemia. This condition not only affects the physical well-being of the donors but also leads to a decline in their willingness to donate blood. Blood collection and supply institutions should enhance their focus on the iron nutritional status of plateletpheresis donors and implement various measures, such as intensifying health education regarding the significance of iron supplementation, implementing programs for testing iron deficiency, considering the provision of iron supplements and extending blood donation intervals. It is crucial to prevent iron deficiency in plateletpheresis donors. These institutions should explore calculation models that can predict personalized blood donation intervals and iron supplementation strategies, and seek a balanced approach that is optimal for maintaining adequate collections while safeguarding donor health. The article comprehensively reviews literature at home and abroad on the etiology and hazards of iron deficiency in plateletpheresis donors, as well as detection methods and response measures. It serves as a foundation for developing scientific and reasonable care measures for blood donation, while also achieving personalized and scientific management and recruitment strategies for blood donors.

Analysis of hematological parameters in male plateletpheresis donors / 中国输血杂志

【Objective】 To investigate the variation of hematological parameters in male plateletpheresis donors. 【Methods】 A total of 194 male plateletpheresis donors from Fujian Blood Center were divided into two groups according to the frequency of blood donation: Group 1 (n=107), with the number of plateletpheresis donation less than or equal to 12 per year; Group 2 (n=87), with the number of plateletpheresis donation more than 12 per year. Serum ferritin (SF) and related iron metabolism indexes, red blood cell count (RBC), hemoglobin (Hb), hematocrit(Hct), platelet count (Plt) and other blood routine indexes, as well as percentage of reticulocyte counts (RET%), immature reticulocyte fraction(IRF) and other reticulocyte indexes were measured before blood donation and analyzed by statistical methods. 【Results】 Compared with Group 2, the RBC, Hb, Hct, SF in Group 1 were significantly higher, while Plt, RET%and IRF were significantly lower(P<0.05), and the probability of ferritin decrease in Group 1 was lower, with significant difference(P<0.05). 【Conclusion】 As the number of donation increased, male plateletpheresis donors were prone to iron deficiency, and the bone marrow hematopoiesis were obviously enhanced. We should be more concerned about male plateletpheresis donors who donated more than 12 times per year, further more, SF monitoring should be conducted and the blood donation interval should be appropriately extended.

Predicting donor-related factors for high platelet yield donations by classification and regression tree analysis

Introduction Collecting high-dose (HD) or double-dose (DD) apheresis platelets units from a single collection offers significant benefit by improving inventory logistics and minimizing the cost per unit produced. Platelet collection yield by apheresis is primarily influenced by donor factors, but the cell separator used also affects the collection yield. Objectives To predict the cutoff in donor factors resulting in HD and DD platelet collections between Trima/Spectra Optia and MCS+ apheresis equipment using Classification and Regression Trees (CART) analysis. Methods High platelet yield collections (target ≥ 4.5 × 1011 platelets) using MCS+, Trima Accel and Spectra Optia were included. Endpoints were ≥ 6 × 1011 platelets for DD and ≥ 4.5 to < 6 × 1011 for HD collections. The CART, a tree building technique, was used to predict the donor factors resulting in high-yield platelet collections in Trima/Spectra Optia and MCS+ equipment by R programming. Results Out of 1,102 donations, the DDs represented 60% and the HDs, 31%. The Trima/Spectra Optia predicted higher success rates when the donor platelet count was set at ≥ 205 × 103/µl and ≥ 237 × 103/µl for HD and DD collections. The MCS+ predicted better success when the donor platelet count was ≥ 286 × 103/µl for HD and ≥ 384 × 103/µl for DD collections. Increased donor weight helped counter the effects of lower donor platelet counts only for HD collections in both the equipment. Conclusions The donor platelet count and weight formed the strongest criteria for predicting high platelet yield donations. Success rates for collecting DD and HD products were higher in the Trima/Spectra Optia, as they require lower donor platelet count and body weight than the MCS+.

Melhores práticas no cuidado durante a doação de plaquetas por aférese: protocolo de revisão de escopo / Best care practices during apheresis platelet donation: a scoping review protocol / Mejores prácticas de atención durante la donación de plaquetas por aféresis: un protocolo de scoping review

OBJETIVO: mapear, na literatura científica, as melhores práticas na gestão do cuidado, durante o processo de doação de plaquetas por aférese. MÉTODO: esta revisão de escopo seguirá a metodologia proposta pelo Joanna Briggs, sendo norteada pela questão de pesquisa: Quais as melhores práticas na gestão do cuidado durante o processo de doação de plaquetas por aférese? Serão considerados artigos originais, independente do desenho do estudo; em português, inglês, francês e espanhol; entre os anos 2017 e 2021; que forneçam texto completo. Como critérios de exclusão: estudos relacionados à terapêutica de aférese plaquetária ou pesquisas que envolvam animais. Após seleção dos descritores, será realizada busca prévia nas bases MEDLINE, via PubMed, e CINAHL. Os dados selecionados para revisão serão exibidos no fluxograma PRISMA para revisões de escopo.

OBJECTIVE: to map, in the scientific literature, the best care practices during platelet donation by apheresis. METHOD: this scoping review will follow the Joanna Briggs Institute's framework, guided by the research question: What are the best care practices during platelet donation by apheresis? Original articles will be considered, regardless of the study design, in Portuguese, English, French, and Spanish, published from 2017 to 2021, with full text available. As exclusion criteria: studies related to platelet apheresis therapy or animal research. After selecting the descriptors, a previous search will be conducted in MEDLINE via PubMed and CINAHL. Studies selected for review will be displayed in the PRISMA flowchart for scoping reviews.

Research progress of iron deficiency in apheresis donors / 中国输血杂志

【Objective】 The loss of iron stores is well documented in whole blood donors, and a large number of studies have confirmed that blood donors are at risk of iron deficiency. With the development of apheresis donation, more and more attention has been paid to the health of apheresis donors. However, little is known about the iron status of apheresis donors. In recent 10 years, iron status of apheresis donors has been studied at home and abroad, and iron deficiency was found common among them. There is no systematic study concerning apheresis donors iron deficiency at present. This article summarizes the literature, concerning the principles and preventive measures about iron deficiency in apheresis donors, and aims at improving the recruitment strategy and providing high-quality service for donors.

Changes in hematological parameters of long-term high-frequency platelet donors / 中国输血杂志

【Objective】 To understand the effect of long-term high-frequency platelet donation on the health, safety and platelet quality of blood donors. 【Methods】 From August 2020 to July 2022, blood donors who donated platelets for single collection in the station were selected as two groups: those who donated for 20-29 times and those who donated for 30-44 times. Such 14 test indexes as red blood cell count (RBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), platelet count (Plt), white blood cell count (WBC), large platelet ratio (P-LCR), lymphocyte (LYM) , neutrophil (NE), mean hemoglobin content (MCH), mean hemoglobin concentration (MCHC), platelet specific volume (PCT), mean platelet volume (MPV) and platelet distribution width (PDW) were grouped and statistically analyzed for 5 times in each group. In addition, blood donors who have donated platelets more than 100 times in the station were chosen; the changes of their 5 parameters as RBC, Hb, Hct, PLT and WBC, as well as the correlation with the total number of platelet donations were analyzed through statistical analysis of the first 100 donations(10 donations/group). 【Results】 During 2 years, the hematological parameters were similar between 20-29 donation group(n=30) and 30-44 donation group(n=11) (P>0.05). For donors with donations≥100 occasions, RBC, Hb, Hct and WBC were negatively correlated with the number of blood donations, while Plt was positively correlated. There were significant differences in Hb, Hct, WBC and Plt among groups (P<0.05). Hb, Hct and WBC showed a downward trend, while Plt showed an upward trend. 【Conclusion】 With the increase of blood donations and units of blood donated, some changes in hematological parameters are observed among long-term high-frequency platelet donors. Monitoring and health education should be strengthened to ensure the safety and quality of blood donors.

Factors influencing the returning of plateletpheresis donors / 中国输血杂志

【Objective】 To explore and analyze on the factors that influence the returning of plateletpheresis donors. 【Methods】 A total of 1 302 plateletpheresis donors, selected from 2014 to 2020 in the blood center, were followed up for 1 year, and their re-donations were recorded. The data of factors were analyzed through Univariate analysis and Logistic regression on statistical software. 【Results】 From 2014 to 2020, there were 1 302 donors participated first-time plateletpheresis donation, including 1070 males and 232 females, aged 18-55 years old, with an average age (28.69±12.90) of years old. As to the education level, 539 cases were junior high school education level and below, 435 cases senior school or technical secondary school education level, and 328 cases junior college education level and above. Within the 1 302 first-time platelet donors, 16 developed adverse reactions to blood donation. 455 donors donated one dose, and 847 cases donated two doses. 506 were public or permanent employed, and 796 were unemployed. 1 267 were ethnic Hans and 35 were ethnic minorities. 21.81%(284/1 302) returned to donate within one year. Univariate and multivariate Logistic regression analysis showed that gender, age, educational level, whether suffered adverse reactions during the first-time donation, doses of donation, ethnicity and occupations all affected the returning of plateletpheresis donors. 【Conclusion】 Plateletpheresis donors with younger age, lower education level, unemployed status, ethnic minorities, no adverse reactions occurring during the first-time donation, and donation history of two doses are more likely to return to donate.

Effect of health publicity on plateletpheresis donation among college students in Beijing / 中国输血杂志

【Objective】 To evaluate the effect of health publicity on plateletpheresis donation among college students in Beijing. 【Methods】 Plateletpheresis donor recruitment was carried out in a conventional way in 2016~2017 and by health publicity in 2018~2019 among college students in Beijing. The data, including the number of blood donations, blood collection units, the number of repeated donations, the rate of eligible donors and the discarding rate due to blood donation reactions, of two groups were collected and statistically analyzed. 【Results】 The number of plateletpheresis donation, the collection units of platelets and the number of repeated blood donation among college students in Beijing have increased since 2016. The rate of ineligible college students at pre-donation screening decreased from 10.97% in 2016~2017 to 8.51% in 2018~2019(P<0.05), and the discarding rate due to blood donation reactions decreased from 0.45% to 0.17%(P<0.05). Most plateletpheresis candidates failed in the blood routine testing. 【Conclusion】 Health publicity helped college students in Beijing better understand the process of plateletpheresis donation to a certain extent, which played a positive role in expanding blood donors and promoting the recruitment and retention of apheresis donors in Beijing.

How apheresis donation effects peripheral blood parameters in plateletpheresis donors: Propensity Score Matching method / 中国输血杂志

【Objective】 To investigate the changes of platelet, white blood cell, red blood cell counts, Hb and PCV before and after plateletpheresis donation. 【Methods】 10 444 platelet donors who donated platelet from Aug 1, 2019 to Jul 31, 2020 were selected as subjects, among which first-time donors were selected as control group and donors who donated twice or more as donation group. After matching gender and age using Propensity Score Matching method in the two groups, the changes of platelet, white blood cell, red blood cell counts, Hb and PCV of platelet blood donors were compared by t test. 【Results】 There were a slight increase in Plt (t=-6.651, P0.05) and in PCV (t=0.030, P>0.05) after plateletpheresis donation. 【Conclusion】 Plateletpheresis donation has an effect on some peripheral blood parameters, but within the normal reference range.

Hemoglobin and ferritin status in frequent plateletpheresis donors / 中国输血杂志

【Objective】 To investigate and analyze the effect of frequent platelet donation on hemoglobin(Hb) and ferritin in blood donors. 【Methods】 73 male plateletpheresis donors, with an average donation interval of 15.26 days, in Beijing Red Cross Blood Center between March 2019 to June 2019 were enrolled into this study. These donors were classified into 3 groups according to the ferritin level during the 5 consecutive donations: 1st group (n=21, ferritin ≥30 μg/L), 2nd group (n=23, ferritin<30 μg/L) and 3rd group (n=29, ferritin fluctuated at 30 μg/L). Their Hb and ferritin during the 5 consecutive donation were detected and further analyzed. 【Results】 1) No significant difference was found in Hb during the 5 consecutive donations (P>0.05), and the ferritin decreased gradually(P<0.01). The incidence of low-value ferritin increased with the donations (35.62% - 63.01%). 2) The same change trend in Hb and ferritin was observed in 1st and 2nd group, except for Hb at 4th donation was significantly higher in 1st group than it in 2nd group. 3) Increasing Hb threshold for donation from 120g/L to 135 g/L can reduce the incidence of low-value ferritin from 23.23% to 18.00%, but also lead to a decrease of blood resources by 10.61%. 【Conclusion】 A progressive decline in iron reserve was observed in frequent platepheresis donors, with minimal impact of Hb on reducing the risk of iron deficiency. It is particularly necessary to carry out ferritin testing for frequent platepheresis donors.

Investigation and analysis of plasma ferritin levels in plateletpheresis donors / 中国输血杂志

【Objective】 We performed a prospective analysis of iron status in plateletpheresis donors, to provide data basis and support for optimizing the strategy of plateletpheresis donation and high quality service for donors. 【Methods】 Plasma ferritin levels were measured in samples from 3 444 plateletpheresis donors who donated in Beijing Red Cross Blood Center to evaluate iron status from the perspective of sex, age, donation status and number of donations in the past 12 months. 【Results】 1) The incidence of low ferritin level was 26.63% in 3 444 cases. There was significant difference in the incidence of low ferritin between sex, as 26.75% in males and 26.31% in females (P>0.05), respectively. There was no significant difference in the concentration of ferritin and the incidence of low ferritin between different age groups of first-time donors. The concentration of ferritin declined and the incidence of low ferritin in males and females increased along with accumulated donations during the past 12 months. 【Conclusions】 This is a large study of ferritin testing in Chinese plateletpheresis donors from multiple dimensions, which laid a foundation for further researches. Ferritin screening is necessary and helpful to reduce the risk of iron deficiency in plateletpheresis donors.

Progress of clinical application of autologous plateletpheresis technology in cardiovascular surgery / 中国胸心血管外科临床杂志

@#The incidence of cardiovascular disease remains high, and surgery is an important measure for the treatment of cardiovascular disease. However, cardiovascular surgery is complicated and difficult, and it is one of the departments with the highest rate of allogeneic blood transfusion. Allogeneic blood transfusion significantly increases the complications and mortality of patients, while autologous blood transfusion can effectively reduce allogeneic blood transfusion and adverse reactions. Autologous plateletpheresis technology is a popular autotransfusion method in recent years. This article reviews the autologous plateletpheresis technology and its clinical application in cardiovascular surgery.

Frequent plateletpheresis donations & its effect on haematological parameters: An observational study

Background & objectives: The well-being of donors undergoing frequent plateletpheresis has been a matter of concern. The aim of this study was to analyze the effect of frequent plateletpheresis on the haematological parameters (HP) of repeat donors. Methods: The study was conducted during February 2016 to March 2017 on all the repeat plateletpheresis donors undergoing the 2nd plateletpheresis within a month of the first in a tertiary care centre. Donors repeating plateletpheresis 3rd and 4th times were also studied. The values of the HP observed on follow up after plateletpheresis done on three different separators were compared. Results: HPs of the 98 donors were similar at follow up except mean platelet volume (P <0.05). Of the 98 donors, 35 were followed up within a week and 63 were followed up within 8-30 days. No significant alteration was found in the HPs except a significant difference in the variation of platelet counts of the two groups (P=0.025). In 34 donors who presented 3rd time for plateletpheresis (mean gap between 1st and 3rd plateletpheresis=31 days), no significant differences in the HPs were found except the platelet distribution width (P <0.05). Minimal difference in the HP was found in the baseline and the follow up of 3rd plateletpheresis i.e., at 4th plateletpheresis donation. Plateletpheresis through all the three cell separators used had similar effects on the follow up HPs. Interpretation & conclusions: Repeated plateletpheresis can be done without any detrimental effects on the cell counts of the plateletpheresis donors. The three cell separators yielded similar post-donation follow up haematological parameters.

Donors’ Calcium Level and Bone Density after Frequent and Regular Plateletpheresis Blood Donation

@#Introduction: Citrate is commonly used as an anti¬coagulant during plateletpheresis procedure. The calcium chelating property of citrate may cause hypocalcaemia when the anticoagulated blood are returned to the donor’s circulation after selective removal of platelet. This study aims at investigating how regular plateletpheresis affects calcium level and bone density in the donors. Methods: A cross-sectional study was conducted among healthy donors at National Blood Centre, Kuala Lumpur, from 15th January till 31st March 2016. Donors were divided into two groups based on the frequency of plateletpheresis donation: low frequency group - donors who had donated less than 20 times, high frequency group - donors who had donated more than 50 times. Dual emission X-ray absorptiometry (DEXA) scan was performed to assess bone density. Pre-donation blood sampling was taken for albumin level. Calcium and magnesium levels were measured before and after donation. Results: Fifty donors participated in this study where the median age of participants was 35.0 years for low frequency and 45.2 years for high frequency group. There was no significant difference in the corrected calcium for both groups before and after plateletpheresis. However, the magnesium levels were significantly reduced in both arms (P<0.05). The albumin level and DEXA scan showed no significant differences between the groups. Conclusion: This study did not show any significant difference in calcium levels, albumin and bone density; but donors’ magnesium levels were reduced in low and high frequency donors after plateletpheresis.

Observation on the effect of calcium gluconate oral solution combined with psychological intervention on preventing the advertise reaction in blood donation by plateletpheresis / 中国生化药物杂志

Objective To study the effect of calcium gluconate oral solution combined with psychological intervention on preventing the advertise reaction in blood donation by plateletpheresis.Methods From February 2015 to February 2016, 114 cases were collected in Shaoxing downtown blood bank, and were divided into the control group and the experimental group 57 cases in each group.In the blood collection process, the control group were not given any treatment, the experimental group was given calcium gluconate oral liquid combined with psychological intervention.The total incidence adverse reactions and SAS scores in the two groups was compared.Results Before blood donation, Before blood donation, SAS scores in the two groups has no statistically significance.After blood donation, the SAS scores and the total incidence of adverse reactions in the experimental group were significantly lower than those in the control group, the differences in the two groups were statistically significant.(P<0.05).Conclusion Calcium gluconate oral liquid combined with psychological intervention can prevent the adverse reactions in the process of blood donation by plateletpheresis, which is worthy of promotion in the process of plateletpheresis.

Influence of acute plateletpheresis on coagulative function after complex aortic arch surgery with deep hy-pothermic circulatory arrest / 临床麻醉学杂志

Objective This study evaluates the effectiveness for using acute plateletpheresis (APP) as a blood conservation method to reduce the need of blood transfusion and increase coagulation function in aortic arch surgery with deep hypothermic circulatory arrest (DHCA ). Methods Thirty-six type-A aortic dissections patients (male 31,female 5,age 23-65 years,ASA physical status II-IV)undergoing frozen elephant trunk with total arch replacement (Bentall plus Sun's surgery)were enrolled in the prospective randomized trial.The patients were randomized into two groups:regular blood conservation group (group control,n = 18)and group APP (n = 18).Blood sample was collected respectively after anesthesia induction (T1 ),before heparinization (T2 ),by the end of surgery (T3 )and 24 hours after surgery (T4 ).Data was collected and reviewed in terms of perioperative transfusion needs,normal laboratory examination,clinical outcomes including blood routine analysis (Hb,Plt,MPV,P-LCR)and thrombelastography (TEG-R,TEG-K,TEG-α,TEG-A,TEG-MA,TEG-EPL).Kaolin and heparinase detections were performed for TEG.Results Com-pared with T1 ,TEG-R,TEG-K,TEG-A,TEG-MA,TEG-EPL and Plt were significantly decreased while TEG-CI,MPV,P-LCR significantly increased in T4 in group APP (P <0.05 ).TEG-A,TEG-MA and Plt were significantly greater(P <0.05)in group APP than in group control at T2 ,and TEG-K,TEG-ELP and HBG were significantly less (P <0.05)in group APP than in group control at T3 . Conclusion The utilization of APP technique was associated with the improved coagulation function in aortic arch surgery with DHCA.