RESUMO
Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun (CK), though its oral bioavailability in rat is extremely low (0.69%) and its in vivo and in vitro metabolism has not yet been systematically investigated. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was employed to identify the metabolites and investigate the metabolic pathways of poliumoside in rat after oral administration 1.5 g·kg of poliumoside. As a result, a total of 34 metabolites (30 from urine, 17 from plasma, and 4 from bile) and 9 possible metabolic pathways (rearrangment, reduction, hydration, hydrolyzation, dehydration, methylation, hydroxylation, acetylation, and sulfation) were proposed in vivo. The main metabolite, acteoside, was quantified after incubated with rat intestinal bacteria in vitro. In conclusion, the present study systematically explored the metabolites of poliumoside in vivo and in vitro, proposing metabolic pathways that may be significant for further metabolic studies of poliumoside.
Assuntos
Animais , Masculino , Ratos , Administração Oral , Bactérias , Metabolismo , Bile , Química , Ácidos Cafeicos , Sangue , Química , Urina , Callicarpa , Química , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Química , Metabolismo , Glicosídeos , Sangue , Química , Urina , Intestinos , Microbiologia , Espectrometria de Massas , Métodos , Estrutura Molecular , Plasma , Química , Ratos Sprague-Dawley , Urina , QuímicaRESUMO
Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun (CK), though its oral bioavailability in rat is extremely low (0.69%) and its in vivo and in vitro metabolism has not yet been systematically investigated. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was employed to identify the metabolites and investigate the metabolic pathways of poliumoside in rat after oral administration 1.5 g·kg of poliumoside. As a result, a total of 34 metabolites (30 from urine, 17 from plasma, and 4 from bile) and 9 possible metabolic pathways (rearrangment, reduction, hydration, hydrolyzation, dehydration, methylation, hydroxylation, acetylation, and sulfation) were proposed in vivo. The main metabolite, acteoside, was quantified after incubated with rat intestinal bacteria in vitro. In conclusion, the present study systematically explored the metabolites of poliumoside in vivo and in vitro, proposing metabolic pathways that may be significant for further metabolic studies of poliumoside.
Assuntos
Animais , Masculino , Ratos , Administração Oral , Bactérias , Metabolismo , Bile , Química , Ácidos Cafeicos , Sangue , Química , Urina , Callicarpa , Química , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas , Química , Metabolismo , Glicosídeos , Sangue , Química , Urina , Intestinos , Microbiologia , Espectrometria de Massas , Métodos , Estrutura Molecular , Plasma , Química , Ratos Sprague-Dawley , Urina , QuímicaRESUMO
Objective: To study the technology for separation and purification of phenylethanoid glycosides from Callicarpa kwangtungensis by macroporous resin. Methods: The absorption and separation properties of 10 kinds of absorption resins were studied and the separation and purification technological process of phenylethanoid glycosides from C. kwangtungensis were optimized by HPLC with the total content of forsythiaside B and poliumoside as an index. Results: Using X-5 resin column as adsorbent, the optional conditions were as follows: loading qualities were 32.79 mg/g, the resin was washed by 4 BV distilled water to remove impurity and 7 BV 30% ethanol to elute phenylethanoid glycosides, the eluting velocity was 2 BV/h, and the content of phenylethanoid glycosides in the extract was 52.8% (n = 3). Conclusion: This technology is simple and feasible, and the process with X-5 resin is an effective method to separate and purify phenylethanoid glycosides from C. kwangtungensis.