Research Status of Endothelialization of Poly （ε-caprolactone） Small-Caliber Vascular Graft Implanted in Animals / 医用生物力学

The most serious problem facing the implantation of vascular graft in the body is the formation of blood clots. In order to solve this problem, various attempts have been made by the scientific community for many years. However, endothelialization is the fundamental method to solve thrombosis and keep vascular graft open for a long time. Poly （ε-caprolactone） (PCL) has the advantages of biodegradability, low cost and good mechanical properties. In recent years, it has been widely used as tissue engineering scaffolds, drug deliverys and so on. This article mainly reviews the endothelialization of small-caliber vascular graft based on PCL after implanted in different animal models, as well as the endothelialization of the same animal model but under different implantation conditions, and trying to find the reasons why small-caliber vascular grafts are still not ideal in clinical applications at different angles such as the different animal models and the different way about endothelialization, and provide references for future animal model selection.

Effect of Zebularine loaded MePEG-PCL nanoparticles on viability, attachment of in vitro cultured lens epithelial cells / 国际眼科杂志(Guoji Yanke Zazhi)

Abstract?AlM: To investigate the effect of zebularine ( Zeb ) loaded Poly ( ethylene glycol ) - block - poly ( ε -caprolactone) methyl ether ( MePEG-PCL) nanoparticles ( NPs) on the viability, attachment, and apoptosis of in vitro cultured lens epithelial cells ( LECs) .?METHODS: In vitro cultured infant human lens tissue HLE B-3 immortalized cells were distributed randomly divided into six groups. Each group was administered with free Zeb 50μmol/L ( ZebF1 group ) , 100μmol/L ( ZebF2 group) , Zeb -loaded MePEG-PCL NPs 50μmol/L ( ZebNP1 group) , Zeb -loaded MePEG-PCL NPs 100μmol/L ( ZebNP2 group) , MePEG-PCL empty NPs( NPs group) or blank medium (group C) respectively. A tetrazolium dye assay ( MTT) test and modified MTT test were performed to determine cell viability and cell attachment. DNA ladder was used to detect the cell apoptosis.?RESULTS: Determined by MTT colorimetric method:Cell proliferation rate of LECs were suppressed by all Zeb administration groups in a concentration-time dependent manner (P ZebNP1>ZebF2 (P<0. 05).?CONCLUSlON: Zeb loaded MePEG-PCL NPs had better effect on suppressing the viability and attachment of in vitro cultured LECs than the free Zeb groups , as well as enhancing the apoptosis.

Formulation and evaluation of nanoparticles containing artemisinin HCL.

The present study deals with the formulation and evaluation of Artemisinin HCl nanoparticles. Artemisinin is a sesquiterpene lactone chemical extract from Artemisia annua (sweet wormwood), is poorly soluble in water and a fast-acting blood schizonticide effective in treating the acute attack of malaria (including chloroquine – resistant and celebral malaria). Artemisinin are effective against multi-resistant strains of P. falciparum. The purpose of the present work is to minimize the dosing frequency, taste masking and toxicity and to improve the therapeutic efficacy by formulating Artemisinin HCl nanoparticles. Artemisinin HCl nanoparticles were formulated by solvent evaporation method using polymer poly(ε-caprolactone) with five different formulations. Nanoparticles were characterized by determining its particle size, polydispersity index, drug entrapment efficiency, particle morphological character and drug release. The particle size ranged between 100nm to 240nm. Drug entrapment efficacy was > 99%. The in-vitro release of nanoparticles were carried out which exhibited a sustained release of Artemisinin HCl from nanoparticles up to 24hrs. The results showed that nanoparticles can be a promising drug delivery system for sustained release of Artemisinin HCl.

Paclitaxel loaded poly(ε-caprolactone)/poloxamer 188 blend microspheres and in vivo evaluation of its antitumor activity / 国际生物医学工程杂志

Objective To prepare paclitaxel-loaded poly(ε-caprolactone)(PCL)/pluronic F68(F68)blend microspheres as a controlled release system. Methods Paclitaxel-loaded PCL/F68 blend microspheres were prepared by the oil-in water(O/W)emulsion/solvent evaporation method. Characterization of the microspheres followed to examine the particle size, the drug encapsulation efficiency, the surface morphology, in vitro release behavior and DSC analysis. In vivo antitumor activity of paclitaxel-loaded PCL/F68 blend microspheres was evaluated in mice bearing with hepatoma H22 cells ascites tumor. Results The results showed that the porous structure can be formed in the surface of PCL/F68 blend microspheres. Faster and controlled release of paclitaxel from PCL/F68 blend microspheres was achieved in comparison with the PCL microspheres. In animal tests, paclitaxel-loaded PCL/F68 blend microspheres showed the potent antitumor activity against hepatoma H22 cells in ascites tumor model. Conclusion The paclitaxel loaded PCL/F68 blend microspheres were found to own a faster release rate and a remarkably controlled release behavior.