RESUMO
Objective To explore the optimal conditions for extraction of Polygala fallax Hemsl. Methods Various trials were conducted to extract P. Fallax Hemsl. With L_(16) (4~5) orthogonal experiments. Results The optimal experimental parameters for extraction were 95% ethenol, dosage-liquor ration at 1:5, three-times extraction at 75℃, 1.5 hours for each extraction, and extraction rate at 11.89%. Conclusion Orthogonal test is convenient and accurate in extracting P. Fallax Hemsl.
RESUMO
Objective To investigate the chemical constituents of Polygala fallax.Methods The chemical constituents were isolated and purified via silica gel and Sephadex LH-20 column chromatography as well as recrystallization.Their structures were determined on the basis of spectral analysis and physicochemical properties.Results Six compounds were isolated and identified as 3-O?-D-glucopyranosyl senegenic acid(Ⅰ),tenuifolin(Ⅱ),presenegenin(Ⅲ),1,7-dimethoxy-2,3-methylenedioxy xanthone(Ⅳ),sinapinic acid(Ⅴ),ferulic acid(Ⅵ).Conclusion Compounds Ⅲ—Ⅵ are isolated from P.fallax for the first time.Compound Ⅰ is a new triterpenoid saponin,named fallaxsaponin A.
RESUMO
AIM: To select the macroresin used for purification of total saponins of Polygala Fallax Hemsl. METHODS: The adsorption and desorption capability of five different macroresins LSA-7,LSA-10,LSA-30,D-101A and HPD-100 in static state were studied by UV. RESULTS: nonpolar LSA-30 and HPD-100 macroporus resin had the optimum comprehensive properties of adsorption and desorption. CONCLUSION: The experimental results provide the basis for selection of macroporus resins used to purify total saponins of Polygala Fallax Hemsl.