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Obiective To investigate the chemical constituents of Pothos chinensis.Methods Compounds were isolated by the silica gel and Sephadex LH-20,semi-PHPLC,preparative thin layer chromatography and recrystallization.Structures of the com-pounds were identified by the physicochemical properties as well as the 1H NMR,13C NMR and MS spectroscopic data.Results Six-teen compounds were isolated from the whole plant of P.chinensis and their structures were identified as liquiritigenin(1),p-hydroxy-benzoic acid(2),syringic acid(3),E-4-hydroxycinnamic acid(4),N-trans-p-coumaroyltyramine(5),N-trans-cinnamoyltyramine (6),N-trans-feruloyltyramine(7),(+)-epiloliolide(8),neoechinulin A(9),vitexin(10),berchemolide(11),(±)-syringaresinol (12),prunasin(13),4-hydroxy-3,5-dimethoxybenzaldehyde(14),β-sitosterol(15)and β-daucosterol(16),respectively.Conclu-sion Compounds 1 and 9-14 are isolated from the genus Pohos for the first time.
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OBJECTIVE:To screen the α-glucosidase inhibitory active part from Pothos chinensis. METHODS:The aqueous extractions of P. chinensis were extracted by petroleum,ethyl acetate,n-butyl alcohol in turn to obtain different polarparts. Effect of each part on α-glucosidase inhibitory activity was determined,and enzyme inhibition kinetics was conducted for the screened parts with strong activity and relatively high yield rate;effects of each part on blood glucose level of mice loaded with glucose,su-crose and starch were respectively determined (using Acacoside tablet as positive control). RESULTS:Enzyme inhibition kinetics in vitro showed the ethyl acetate part [yield rate was 0.40%,enzyme activity inhibition rate was(72.90±2.85)%] had strongα-glu-cosidase inhibitory activity and showed a dose-dependent,fast,non-competitive and reversible model. Results of in vivo glucose tol-erance indicated that Acacoside tablet and each part of P. chinensis had no effects on blood glucose level of glucose-loaded mice (P>0.05);while Acacoside tablet and ethyl acetate part in P. chinensis could reduce 30,60 min blood glucose level of su-crose-loaded mice and 30,60,120 min blood glucose level of starch-loaded mice(P<0.05 or P<0.01). CONCLUSIONS:Ethyl acetate part is theα-glucosidase inhibitory active part from Yao medicine P. chinensis.
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OBJECTIVE: To investigate the liposoluble constituents from the herb of Pothos chinensis (Raf.) Merr. of Araceae. METHODS: The compounds were isolated and purified by silica gel, Sephadex LH -20, semi-preparative HPLC and recrystallization methods. Their structures were identified on the basis of spectral analysis, physicochemical characteristics and comparison of their spectral data with those in the literature. RESULTS: Seventeen compounds were isolated and identified respectively as honokiol(1), hinokinin(2), racemosol(3), 24-propylcholest-7-en-3-ol(4), β-sitosterol(5), daucosterol (6), hentriacontane(7), palmitic acid (8), oleic acid(9), linoleic acid(10), 9, 12, 15-ocatadecatrienoic acid(11), 13-phenyltridecanoic acid(12), 13-(3', 4'-methyl -enedioxyphenyl)-tridecanoic acid(13), 1-monostearin(14), 1-monopalmitin(15), benzoic acid(16), and vanillic acid(17). CONCLUSION: Compounds 1-16 are isolated for the first time from this plant.
RESUMO
Objective: To investigate the chemical constituents in the whole herbs of Pothos chinensis. Methods: The chemical constituents were isolated and purified by various column chromatographic methods and preparative HPLC. Their structures were identified by physicochemical properties and spectral analyses. Results: Seventeen compounds were isolated from the methanol extract and identified as N-trans-cinnamoyltyramine (1), N-trans-p-coumaroyltyramine (2), N-cis-p-coumaroyltyramine (3), N-trans- feruloyltyramine (4), N-cis-feruloyltyramine (5), loliolide (6), dehydrovomifoliol (7), (3S, 5R, 8R, 9R)-5, 8-epoxy-6-megastigmene-3, 9-diol (8), (3R, 6R, 7E)-3-hydroxy-4, 7-megastigmadien-9-one (9), (3R)-4-[(2R, 4S)-2-hydroxy-2, 6, 6-trimethylcyclohexylidene]-3- buten-2-oneiol (10), 7-methoxycoumarine (11), imperatorin (12), 7-hydroxy-8-methoxycoumarin (13), 6-hydroxy-7-methoxycoumarin (14), indole-3-aldehyde (15), (-)-medioresinol (16), and daidzein (17). Conclusion: All the compounds are isolated from the plants in Pothos L. for the first time.