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Objective@#To investigate the comparative study of serum hepatitis B virus (HBV) large protein (HBV-LP) , HBV-DNA, and Pre S1 antigen (Pre S1-Ag) detection in the evaluation of serum HBV replication in patients with chronic hepatitis B.@*Methods@#A total of 482 patients infected with chronic hepatitis B virus (CHB) were enrolled and the serums were collected in a hospital of Hefei city in Anhui province from June 2013 to March 2015. The serum HBV-LP, HBV markers(HBV-M) and Pre S1-Ag were detected using ELISA, and HBV-DNA were quantified using quantitative real-time PCR. The positive detection rate difference of HBV-DNA, HBV-LP and Pre S1-Ag were compared, the correlation between the logarithm of HBV-DNA copies number and the absorbance value of HBV-LP was analyzed using Spearman rank correlation.@*Results@#The positive rates of HBV DNA, HBV-LP, and Pre S1-Ag were 67.22% (324/482), 73.86% (356/482), and 37.34% (180/482), respectively (P<0.01). The positive rates of the three markers were 54.57% (185/339), 64.90% (220/339), and 27.73% (94/339), respectively, in 339 HBeAg-negative CHB patients (P<0.01). In HBeAg negative patients, the positive rate of HBV-LP in 185 cases of HBV-DNA positive samples was 90.81% (168/185), which was higher than that of Pre S1-Ag with rate of 39.46% (73/185) (P<0.01).The positive rate of HBV-LP was 33.77% (52/154) in 154 cases of patients with negative HBV-DNA whose positive rate was higher than Pre S1-Ag with positive rate of 13.64% (21/154)(P<0.01). The positive rates of HBV-DNA, HBV-LP and Pre S1-Ag in HBsAg, HBeAg and HBcAb positive groups were 97.04% (131/135), 94.81% (128/135), and 60.00% (81/135), (P<0.01); The positive rates of three indexes of HBsAg, HBeAb, HBcAb positive group were 53.74% (122/227), 63.88% (145/227), and 27.31% (62/227); The positive rates of three indexes of HBsAg and HBcAb positive group were 55.79% (53/95), 67.37% (64/95), and 28.42% (27/95) (P<0.01). The absorbance value of HBV-LP was positively related with the logarithm of HBV-DNA copies number (Spearman rank correlation coefficient was 0.908, P<0.01).@*Conclusion@#There was a good correlation between HBV-LP and HBV-DNA load value, and could be used as an effective complement for the detection of HBV-DNA and HBV-M. Compared with Pre S1-Ag.
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Objective To investigate the relationship between Pre-S1 antigen and hepatic function of the patients with chronic hepatitis B.Methods Serum samples of 300 cases with chronic hepatitis B patients were collected,Pre-S1 antigen and alanine aminotransferase (ALT),aspartate aminotransferase (AST) were checked,and the correlation were analyzed.Results Among 300 cases,patients with positive Pre-S1 antigen were 58.33％(175/300).In patients with positive Pre-S1 antigen,the abnormal ALT(＞40 U/L) detection rate was 78.75％ (138/175),the abnormal AST(＞40 U/L) detection rate was 77.17％ (136/175),Pre-S1 antigen negative patients ALT abnormal detection rate was 51.20％ (64/125),AST anomaly detection rate was 42.40％ (53/125).There was significant difference between two groups(x2 =2.875,5.223;P＜0.05).Conclusion In patients with chronic hepatitis B,Pre-S1 antigens can be used as a marker of hepatitis B virus infection,copy and hepatic cell injury.
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Objective: To study the correlation between HBV Pre-S1 antigen, HBeAg levels and HBV DNA copies, so as to assess the clinical value of Pre-S1 in detection of HBV replication. Methods: A total of 363 HBsAg-positive samples were collected. The levels of Pre-S1 antigen, HBeAg and HBV DNA copies were determined by ELISA, time-resolved immuno-fluorescent method and real-time fluorescent quantitative PCR(FQ-PCR),respectively. The correlation between the determination results was analyzed. Results: Pre-S1 antigen level was correlated with the level of HBeAg (χ2 = 94.4,P<0.01), with the coefficient being 0.45; Pre-S1 antigen level was also correlated with HBV DNA(χ2 = 198.58, P<0.01), with the coefficient being 0.59. The positive rate of Pre-S1 increased with HBV DNA copies. When logarithm of HBV DNA copies was more than 3, the sensitivity, specificity, accuracy, positive predictive value(PPV), positive likelihood ratio(PLR) and odds ratio(OR) of Pre-S1 were 84.5%,91.2%,87.0%,94.1%,9.6,and 56.8 respective; the numbers of HBeAg were 50.4%,94.9%,67.2%,94.2%, 9.8 and 18.9, respectively. Conclusion: Pre-S1 antigen is correlated with HBV DNA and is more sensitive and accurate than HBeAg in reflecting HBV replication. The above 3 parameters can complement each other and the combination of them is more accurate in clinical diagnosis.
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Objective To discuss the value of detection of Pre S1 antigen(Pre-S1 Ag) of hepatitis B virus. Methods ELISA was used to detect Pre-S1 Ag and HBeAg in serum from 854 HBsAg-positive persons in medical ex-amination who have no symptom. In addition, Pre-S1Ag in serum from 300 random samples of HBsAg-negative persons were detected in medical examination. Results Pre-S1Ag positive rate was 41.1% (351/854) in 854 samples with HBsAg(+) ,and HBeAg positive rate was 26.6% (227/854) in them. Pre-S1 Ag positive rate was 79.7% (181/227) in HBeAg(+) group but was 27.1% (170/627) in HBeAg(-) group. Pre-S1 Ag wasn't detected in 300 samples with HBsAg(-). Conclusions In HBsAg-positive samples who have no symptom,the detection rate of Pre-SIAg is higher than that of HBeAg. Pre-Sl Ag can reflect duplication and infectivity of HBV better than HBeAg and it has more value to epidemiology investigation and clinical diagnosis than the latter.
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Objective To analyze and compare the Pre-S1 antigen and the serum markers to discuss the clinical significance of the Pre-S1 antigen of HBV.Methods The serum markers and the Pre-S1 antigen of HBV were detected by ELISA.Result By analyzing the serum markers in different combination modes,we found that the detection rate of the Pre-S1 antigen in different combination modes are:94% ( in the HBeAg positive mode) ; 15.6%(in the HBeAg negative mode) and negative in the mode both HBsAg and HBeAg are negative.Conclusions The Pre-S1 antigen of HBV has a coherence with HBeAg to a large extent,and has contact relations with the exist and replicate of HBV and can reflect the replication of HBV.The detection of the Pre-S1 antigen can help the diagnosis of HBV infection.
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Objective To investigate the correlation among HBV infection models,HBV Pre-S1 antigen and HBV DNA loads in serum and to provide evidence for the diagnosis and treatment of hepatitis B.Methods Hepatitis B markers,HBV Pre-S1 antigen and HBV DNA loads in serum samples were detected simultaneously for 1037 cases.Results The positive rate of HBV Pre-S1 antigen was higher in HBsAg,HBeAg and HBcAb positive model than that in other models(P0.05).Conclusion The quantitative detection of HBV DNA is important for the prevention of HBV infection.The combination assay of serum HBV markers,Pre-S1 antigen and HBV DNA load was beneficial to the monitoring of disease condition of hepatitis B and to the improvement of therapeutic effect.
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To study the relationship between serum Pre-S1 antigen and HBV DNA, serum Pre-S1 antigen and HBV DNA marker in 229 patients positive for HbsAg were detected by using ELISA method and compared. It was found that both Pre-S1 antigen and HbeAg were correlated with HBV DNA with the coefficient being 0. 9812 and could be used to reflect the existence or reproduction of HBV DNA well. Although there was statistical correlation between them, their clinical implication was not completely the same. Therefore, a conclusion was drawn that combined detection of Pre-S1 antigen, HBV and HBV DNA in the patients with different kinds of HBV infection was helpful to clinical diagnosis, therapeutic effect evaluation and prognosis judgement.
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Objective:To study the correlation between HBV Pre-S1 antigen,HBeAg levels and HBV DNA copies,so as to assess the clinical value of Pre-S1 in detection of HBV replication.Methods:A total of 363 HBsAg-positive samples were col- lected.The levels of Pre-S1 antigen,HBeAg and HBV DNA copies were determined by ELISA,time-resolved immuno-fluores- cent method and real-time fluorescent quantitative PCR(FQ-PCR),respectively.The correlation between the determination re- sults was analyzed.Results:Pre-S1 antigen level was correlated with the level of HBeAg(X~2=94.4,P