RESUMO
Objectives: To explore the effects of uric acid on expression of lysyl oxidase (LOX) and matrix metalloproteinase-2 (MMP-2) in rat vascular smooth muscle cells. Methods: Rat vascular smooth muscle cell was cultured, and divided into following groups: control group, uric acid group (cells were treated with 20, 40, 60 mg/L uric acid for 48 hours, cells were treated with 40 mg/L uric acid for 24, 48, 72 h) and β-aminopropionitrile group (cells were treated with 10 mg/ml β-aminopropionitrile for 24 hours). The reactive oxygen species was detected by confocal microscopy. mRNAs and protein expression levels of LOX and MMP-2 in rat vascular smooth muscle cells were measured by RT-PCR and Western blot, respectively. Results: The number of increased cell proliferation, reactive oxygen species burst, mRNAs and protein expression levels of LOX and MMP-2 were significantly increased in uric acid group than in control group (P<0.01). The mRNAs and protein expression levels of LOX and MMP-2 were significantly downregulated in β-aminopropionitrile group than in uric acid group (P<0.01). Conclusions: Uric acid can enhance the expression of LOX and MMP-2 in rat vascular smooth muscle cells.
RESUMO
Objective To study the effects of atorvastatin on expression of lysyl oxidase(LOX) in myocardial tissue of rats with diabetic cardiomyopathy (DCM) and its mechanism.Methods Thirty DCM SD rats were randomly divided into 3 groups:DCM group,treatment group (atorvastain 2 mg · kg-1 · d-1 by gastric gavage) and β-aminopropionitrile group(β-aminopropionitrile 80 mg · kg-1 · d-1 by gastric gavage),10 cases in each group.Other 10 SD rats were selected as the control group.At the end of week 8,the rats were killed for extracting the myocardial tissue RNA and protein.Expression levels of LOX,MMP-2 and NF-κB mRNAs and proteins in myocardial tissue of DCM rats were measured by RT-PCR and Western blot.Results The expression levels of LOX,MMP-2 and NF-κB mRNAs and proteins in the DCM group were significantly higher than those in the control group (P＜0.01),and compared with the DCM group,the expression of BAPN LOX,MMP-2 and NF-κB mRNA and proteins in the treatment group were significantly deceased (P＜0.01).Conclusion Atorvastatin can reverse the expression of LOX in myocardial tissue of DCM rat,and then may regulate the expression of MMP-2 and NF-κB.