Projection-Specific Heterogeneity of the Axon Initial Segment of Pyramidal Neurons in the Prelimbic Cortex / 神经科学通报·英文版

The axon initial segment (AIS) is a highly specialized axonal compartment where the action potential is initiated. The heterogeneity of AISs has been suggested to occur between interneurons and pyramidal neurons (PyNs), which likely contributes to their unique spiking properties. However, whether the various characteristics of AISs can be linked to specific PyN subtypes remains unknown. Here, we report that in the prelimbic cortex (PL) of the mouse, two types of PyNs with axon projections either to the contralateral PL or to the ipsilateral basal lateral amygdala, possess distinct AIS properties reflected by morphology, ion channel expression, action potential initiation, and axo-axonic synaptic inputs from chandelier cells. Furthermore, projection-specific AIS diversity is more prominent in the superficial layer than in the deep layer. Thus, our study reveals the cortical layer- and axon projection-specific heterogeneity of PyN AISs, which may endow the spiking of various PyN types with exquisite modulation.

The speed-limiting role in the onset of antidepres-sants:the re-establishment of the excitation/inhibi-tion(E/I)function balance in the mPFC of rats / 中国药理学与毒理学杂志

OBJECTIVE There are serious hazards in depression,and the precise mechanism underlying the delayed onset of clinical antidepressants remains unclear.The purpose of this study was to investigate the regular pattern of the speed-limiting role of excitation/inhibition(E/I)function balance in the mechanism of antidepressant action.METHODS Based on the previous study,we focused on glutamatergic pyramidal neurons in the medial prefrontal cortex(mPFC)here and used its excitability to represent the establishment of a new E/I functional balance.We studied the changes in the firing activity of glutamatergic pyramidal neuron in the mPFC at different administration times for five types of antidepressants that act on different pharmacological targets and different onset times,including fluoxetine(SSRI),duloxetine(SNRI),vilazodone[serotonin 1A receptor(5-HT1A)ago-nist and SSRI],ketamine[N-methyl-D-aspartate(NMDA)receptor antagonist],and hypidone hydrochloride(YL-0919,new antidepressant with sigma-1 receptor ago-nist and SSRI).We first examined the initial onset time of activation of pyramidal neurons using multichannel elec-trophysiological recordings and tested the antidepressant behavioral effects using the FST.We then selected three antidepressants(fluoxetine,ketamine,and vilazodone)to explore its effects on the BDNF-mTOR pathway by West-ern blotting.In addition,we disrupted the E/I function bal-ance using chemogenetics to investigate the antidepres-sant-like effects of YL-0919 and ketamine in the FST and TST.RESULTS We found that treatment with fluoxetine for 17 days significantly increased the firing activity of pyramidal neurons and decreased the immobility duration in the FST.Similarly,it took duloxetine for 10 d,vilazodone for 4 d,YL-0919 for 3 d and ketamine for 24 h,to exert such effects.Meanwhile,Western blotting results sug-gested that the expression of BDNF and phosphorylation of mTOR in the mPFC significantly increased.How-ever,haloperidol,a classic antipsychotic(without antide-pressant effects),exerted no such effects on the firing activities of pyramidal neurons.In addition,disrupting the E/I function balance(via activating the GABA neurons and inhibiting the glutamate neurons)blocks out the antidepressant-like effects of YL-0919 and ketamine in the FST and TST.CONCLUSION Taken together,our findings suggest that the commencement of antide-pressant effects may be accompanied by the increase in the firing activity of pyramidal neurons and the activation of the BDNF-mTOR pathway,which may be a necessary and rate-limiting process.The re-establishment of the E/I balance may be a landmark event for the onset of antide-pressant effects.

COVID-19 crítico e disfunção neurológica - uma análise comparativa direta entre o SARS-CoV-2 e outros agentes infecciosos / Critical COVID-19 and neurological dysfunction - a direct comparative analysis between SARS-CoV-2 and other infectious pathogens

RESUMO Objetivo: Avaliar se a infecção grave pelo SARS-CoV-2 está mais comumente associada a sinais de disfunção do trato corticoespinhal e outros sinais, sintomas e síndromes neurológicas, em comparação com outros agentes infecciosos. Métodos: Este foi um estudo de coorte prospectivo com inclusão consecutiva de doentes admitidos a unidades de cuidados intensivos devido a síndrome do desconforto respiratório agudo infeccioso primário, com necessidade de ventilação mecânica invasiva por > 48 horas. Os doentes incluídos foram atribuídos aleatoriamente a três investigadores para a avaliação clínica, a qual incluía a pesquisa de sinais de disfunção do trato corticoespinhal. Os dados clínicos, incluindo outras complicações neurológicas e possíveis preditores, foram obtidos independentemente a partir dos registros clínicos. Resultados: Foram incluídos consecutivamente 54 doentes com síndrome do desconforto respiratório agudo, 27 devido a SARS-CoV-2 e 27 devido a outros agentes infecciosos. Os grupos eram comparáveis na maioria das características. Os doentes com COVID-19 apresentavam risco significativamente superior de complicações neurológicas (RR = 1,98; IC95% 1,23 - 3,26). Os sinais de disfunção do trato corticoespinhal tendiam a ser mais prevalentes em doentes com COVID-19 (RR = 1,62; IC95% 0,72 - 3,44). Conclusão: Este estudo foi a primeira análise comparativa visando avaliar disfunção neurológica, entre doentes com infecção SARS-CoV-2 e outros agentes infecciosos, em um contexto de unidade de cuidados intensivos. Reportamos um risco significativamente superior de disfunção neurológica em doentes com COVID-19. Como tal, sugere-se o rastreio sistemático de complicações neurológicas em doentes com COVID-19 crítico.

ABSTRACT Objective: To evaluate whether critical SARS-CoV-2 infection is more frequently associated with signs of corticospinal tract dysfunction and other neurological signs, symptoms, and syndromes, than other infectious pathogens. Methods: This was a prospective cohort study with consecutive inclusion of patients admitted to intensive care units due to primary infectious acute respiratory distress syndrome requiring invasive mechanical ventilation > 48 hours. Eligible patients were randomly assigned to three investigators for clinical evaluation, which encompassed the examination of signs of corticospinal tract dysfunction. Clinical data, including other neurological complications and possible predictors, were independently obtained from clinical records. Results: We consecutively included 54 patients with acute respiratory distress syndrome, 27 due to SARS-CoV-2 and 27 due to other infectious pathogens. The groups were comparable in most characteristics. COVID-19 patients presented a significantly higher risk of neurological complications (RR = 1.98; 95%CI 1.23 - 3.26). Signs of corticospinal tract dysfunction tended to be more prevalent in COVID-19 patients (RR = 1.62; 95%CI 0.72 - 3.44). Conclusion: Our study is the first comparative analysis between SARS-CoV-2 and other infectious pathogens, in an intensive care unit setting, assessing neurological dysfunction. We report a significantly higher risk of neurological dysfunction among COVID-19 patients. As such, we suggest systematic screening for neurological complications in severe COVID-19 patients.

Clinical features of a pedigree of charcot-marie-tooth disease type 2cc caused by the point mutation of NEFH gene / 中华实用儿科临床杂志

The clinical data of 4 patients in a pedigree of charcot-marie-tooth disease type 2cc (CMT2cc) caused by the NEFH gene mutation from the Department of Rehabilitation, Tianjin Children′s Hospital in March 2020 were reviewed and analyzed retrospectively.The purpose of this study was to improve clinicians′ awareness of the di-sease.The pedigree had signs and symptoms of varying degrees of pyramidal fasciculus involvement, high arched feet, and achilles tendon contracture.The electrophysiological testing of both lower extremities suggested sensory and motor nerve axonal damage, and an abnormal visual evoked potential was observed.Second-generation sequencing revealed that the pathogenic factor was the NEFH gene variation: c.1319G>A (p.Ser440Asn), which is a new mutation site that has never been reported before. NEFH mutations can cause a complex clinical phenotype of CMT2cc, which is therefore easily misdiagnosed.Central and peripheral nerves are simultaneously involved in CMT2cc patients.Electrophysiological testing and genetic analysis are required to clarify the diagnosis of CMT2cc.

Clinical features of a pedigree of charcot-marie-tooth disease type 2cc caused by the point mutation of NEFH gene / 中华实用儿科临床杂志

The clinical data of 4 patients in a pedigree of charcot-marie-tooth disease type 2cc (CMT2cc) caused by the NEFH gene mutation from the Department of Rehabilitation, Tianjin Children′s Hospital in March 2020 were reviewed and analyzed retrospectively.The purpose of this study was to improve clinicians′ awareness of the di-sease.The pedigree had signs and symptoms of varying degrees of pyramidal fasciculus involvement, high arched feet, and achilles tendon contracture.The electrophysiological testing of both lower extremities suggested sensory and motor nerve axonal damage, and an abnormal visual evoked potential was observed.Second-generation sequencing revealed that the pathogenic factor was the NEFH gene variation: c.1319G>A (p.Ser440Asn), which is a new mutation site that has never been reported before. NEFH mutations can cause a complex clinical phenotype of CMT2cc, which is therefore easily misdiagnosed.Central and peripheral nerves are simultaneously involved in CMT2cc patients.Electrophysiological testing and genetic analysis are required to clarify the diagnosis of CMT2cc.

Protein Kinase C Controls the Excitability of Cortical Pyramidal Neurons by Regulating Kv2.2 Channel Activity / 神经科学通报·英文版

The family of voltage-gated potassium Kv2 channels consists of the Kv2.1 and Kv2.2 subtypes. Kv2.1 is constitutively highly phosphorylated in neurons and its function relies on its phosphorylation state. Whether the function of Kv2.2 is also dependent on its phosphorylation state remains unknown. Here, we investigated whether Kv2.2 channels can be phosphorylated by protein kinase C (PKC) and examined the effects of PKC-induced phosphorylation on their activity and function. Activation of PKC inhibited Kv2.2 currents and altered their steady-state activation in HEK293 cells. Point mutations and specific antibodies against phosphorylated S481 or S488 demonstrated the importance of these residues for the PKC-dependent modulation of Kv2.2. In layer II pyramidal neurons in cortical slices, activation of PKC similarly regulated native Kv2.2 channels and simultaneously reduced the frequency of action potentials. In conclusion, this study provides the first evidence to our knowledge that PKC-induced phosphorylation of the Kv2.2 channel controls the excitability of cortical pyramidal neurons.

Training intensity distribution on running time in amateur endurance runners: a scoping review / Distribución de intensidades de entrenamiento sobre el tiempo de carrera en corredores recreativos de resistencia: revisión de alcance

Problem: Intensity in endurance training is important for improving race time; its optimal handling in amateur runners has not been extensively studied. The polarized training intensity distribution (TID) model emerges as a possibility to reduce race time; however, effect of this model remains to be demonstrated compared to other TID models. Objective: The objective of this study is to explore the current state of the evidence and its the gaps, according to the effect of the polarized TID model on race time in amateur runners compared to other TID models. Method: A scoping review without date restrictions was carried out in PubMed, EBSCO, SciELO, LILACS, and Google Scholar. Randomized controlled studies, quasi-experimental studies, and case studies, which comprise polarized TID model in amateur runners on race time, were include. Results: Five studies evaluated the effect on running time using the polarized TID model compared to other models in amateur runners; four of them did not show differences between groups in the race times in two, five, and ten km. Only one study showed significant diferences in the race time at 21 km. Conclusions: The model with polarized TID did not show significant differences in race time compared to other models, except for a case report in which the polarized TID was higher by 21 km compared to the threshold TID: 1 hour. 20 min. 22 seconds and 1 hour. 26 min. 34s, respectively. The scarce evidence found, the heterogeneity in the distances in the evaluated race time, the distribution of zones in the same TID, the duration of the interventions, and the monitoring of the loads, are the main limitations found in the studies. The polarized TID could contribute to adherence, lower perception of effort, and injury prevention. However, this must be tested in future studies.

Problema: La intensidad en el entrenamiento de la resistencia es importante para mejorar el tiempo de carrera; su manipulación óptima en corredores recreativos no ha sido estudiada ampliamente. El modelo de distribución de intensidad del entrenamiento (DIE) polarizado emerge como posibilidad para reducir el tiempo de carrera. Sin embargo, falta demostrar su efecto comparado con otros modelos de DIE. Objetivo: Explorar el estado actual de la evidencia científica y sus vacíos respecto al efecto del modelo de DIE polarizado sobre el tiempo de carrera en corredores recreativos, en comparación con otros modelos de DIE. Método: Se realizó una revisión de alcance sin restricción de fechas en PubMed, EBSCO, SciELO, LILACS y Google Scholar. Se incluyeron estudios controlados aleatorios, estudios cuasiexperimentales y estudios de caso, que tuvieran como DIE el modelo polarizado en corredores recreativos sobre el tiempo de carrera. Resultados: Cinco estudios evaluaron el efecto en el tiempo de carrera usando el modelo de DIE polarizado comparado con otros modelos en corredores recreativos; cuatro de ellos no mostraron diferencias entre grupos en los tiempos de carrera en dos, cinco y diez km. Solo un estudio mostró diferencias significativas en el tiempo de carrera en 21 km. Conclusiones: El modelo con DIE polarizado no mostró diferencias significativas en el tiempo de carrera comparado con otros modelos, a excepción de un reporte de caso en el cual la DIE polarizado fue superior en 21 km comparado la DIE umbral: 1 hora. 20 min. 22 s y 1 hora. 26 min. 34 s, respectivamente. La escasa evidencia encontrada, la heterogeneidad en las distancias en el tiempo de carrera evaluado, la distribución de zonas en una misma DIE, la duración de las intervenciones y la monitorización de las cargas son las principales limitaciones encontradas en los estudios. La DIE polarizado podría contribuir a la adherencia, a una menor percepción del esfuerzo y a la prevención de lesiones. No obstante, esto debe ser probado en estudios futuros.

FOXG1 Directly Suppresses Wnt5a During the Development of the Hippocampus / 神经科学通报·英文版

The Wnt signaling pathway plays key roles in various developmental processes. Wnt5a, which activates the non-canonical pathway, has been shown to be particularly important for axon guidance and outgrowth as well as dendrite morphogenesis. However, the mechanism underlying the regulation of Wnt5a remains unclear. Here, through conditional disruption of Foxg1 in hippocampal progenitors and postmitotic neurons achieved by crossing Foxg1

Separate thyrothymic thyroid remnant; clinically crucial anatomic variation

PURPOSE: The anatomical variations of the thyroid gland including separate thyroidal remnant at the thyrothymic area are of significance during thyroid surgery for “total” thyroidectomy, and for recurrent goitre. In the present study, we aimed to detect the separate rests of thyroidal tissue in the thyrothymic region.METHODS: The thyrothymic region was explored for identification, dissection, and excision of separate thyroidal remnants in 134 patients who underwent primary thyroid surgery. In this series, we studied the incidence and anatomical features of the thyrothymic remnant and its relation with other embryologic remnants.RESULTS: Overall, 222 sides of the thyroid were explored in this study. An entirely separate thyrothymic remnant of the thyroid was identified and excised in 8 of 134 patients (6%). Mean size of removed remnants was 36.4 mm (range, 29–45 mm) in diameter. The incidences of pyramidal lobe (PL) and Zuckerkandl's tubercle (ZT) were 71.6% and 59.7%, respectively. The thyrothymic remnant coexisted with PLs in 4 patients. Four patients had all 3 embryologic remnants: thyrothymic remnant, PLs, and ZTs.CONCLUSION: An entirely separate thyroidal remnant at the thyrothymic area is not a rare variation. The considerably large size of a remnant may threaten the completeness of thyroidectomy and may result in recurrence if it is left behind after thyroid surgery. Awareness, identification, and excision of the separate remnant at the thyrothymic area and the other embryologic remnants are critical for ensuring completeness of thyroidectomy and preventing recurrences.

Morphology of thyroid gland- a comparative study between male and female thyroid glands

Introduction: Thyroid gland surgery is one of the most common surgical interventions in the head and neckregion. The gland is located low down in front of the neck. It consists of two symmetrical lobes united by anisthmus. A small portion of the gland substance often projects upwards from the isthmus, generally to the left ofthe midline as the Pyramidal lobe (PL). The Levator glandulae thyroidea(LGT) descends from the hyoid body to theisthmus or apex of Pyramidal lobe, innervated by branch of external laryngeal nerve. Hence this study onmorphological analysis of the gland will hopefully help to minimise the complications during the surgeries.Materials and methods: The study was done in 50 specimens which included glands dissected out of adultcadavers, specimens used for teaching in the department and rest of them were collected from fresh cadavericworkshops.statistical analysis was done between male and female thyroid glands using spss software 20version, dependant variables were compared using chi square test.Result: The shape of the gland was mostly normal, Isthmus was related to 2,3,4 th tracheal rings and in 6% casesit was absent. The pyramidal lobe was seen mostly to the left in 38% cases, LGT was absent in 36% cases,muscular LGT was seen on left side in 18% cases and STA was seen related to the medial border of apex in 52%cases.Conclusion: In the present study we observed variation in the morphology between the male and female thyroidglands

A Study Of The Variations In The Morphology Of The Thyroid Gland And Its Surgical Correlations

Introduction: Being a highly vascular and one of the essential endocrine glands, the knowledge of the variationsin the morphology of the thyroid gland stands an important role with respect to their identification, isolation orpreservation, and prevention of any untoward complication during the surgeries around the neck region, thusincreasing the chances of favourable surgical outcome.Aim: The aim of the present study was to identify the frequent variants in the structure of the thyroid gland, andto compare the same with the work of other authors.Materials and Methods: The present study was conducted on total 86 thyroid glands with morphological variations,out of which 42 were of males and 44 were of females. These were obtained during routine dissection of the neckin the adult human cadavers aged above 50 years.Results: The most common variation in the present study was the presence of pyramidal lobe (50% in males and65.9% in females), followed by the levator glandulae thyroidae (45.2% in males and 52.3% in females), absenceof the isthmus (7.1% in males and 20.5% in females), accessory thyroid tissue (2.4% in males and 2.8% infemales), and the least common was presence of groove in the thyroid tissue (0% in males and 2.8% in females).There were also combination of variants in some thyroids.Conclusion: The study emphasizes on the common anatomical variations in the thyroid gland morphology andtheir surgical correlations.

Mnemonic and Histopathological Assessment of the Neuroprotective Effects of Murraya Koenigii Leaves Extract in Rats with Partial Global Cerebral Ischaemia

@#Introduction: Preclinical studies have reported that Murraya koenigii leaves (MKL) could enhance memory. MKL is also known for its antioxidant activity. The current study was to assess the possible neuroprotective potential of MKL methanolic extract in a two vessel occlusion (2VO) rat model of partial global cerebral ischaemia. Methods: Rats were divided into memory and learning groups. Each group was subdivided into sham control, untreated 2VO and MKL-treated 2VO subgroups. The Morris water maze test was implemented to assess the rats’ cognitive function postoperatively. Brain samples were histopathologically examined for viable neurons within the CA1 hippocampal region. Results: Water maze test findings showed that MKL positively improved memory and learning impairments. However, this improvement in memory test for the treated group was still significantly inferior to that of the healthy control group. Additionally, MKL treated group exhibited insignificant difference in the number of viable hippocampal CA1 pyramidal neurons from that of the untreated 2VO group, whereas both MKL treated and untreated 2VO groups showed significantly less viable neurons when compared with the control group. Conclusion: MKL extract modestly improved memory without providing substantial neuroprotective action to the hippocampal neurons in rats with chronic partial global cerebral ischaemia.

Anatomical variations of thyroid glands in northeastern-Thai embalmed cadavers / Variaciones anatómicas de la glándula tiroides en cadáveres embalsamados del noreste de Tailandia

SUMMARY: This study aimed to investigate the variation patterns of thyroid glands in embalmed cadavers donated for anatomical studying and research in the northeastern part of Thailand. The crossed-sectional descriptive study was applied to systemically observe the thyroid gland in abnormality of 45 embalmed cadavers. The study was conducted in the Anatomy Laboratory of College of Medicine and Public Health, Ubon Ratchathani University. The indications of thyroid gland abnormalities were recorded by photographs before classification of data used further in a descriptive analysis using SPSS. The results showed that 8 of the 45 cadavers (17.8 %) were observed with thyroid gland abnormality (4 males; 8.9 % and 4 females; 8.9 %). Such variations could be classified into 3 types; pyramidal lobe (8.8 %), levator glandulae thyroideae (2.2 %) and the co-existing of levator glandulae thyroideae (8.8 %), respectively. The thyroid gland abnormality observed in this study was mainly related to many previous reports. Besides the number of thyroid variations are a crucial indication of thyroid gland abnormality recurring, these gland abnormalities are susceptible to the operation of radiology physicians in thyroidectomy and tracheostomy.

RESUMEN: El objetivo de este estudio fue investigar los patrones de variación de la glándula tiroides en cadáveres embalsamados donados para estudios e investigaciones anatómicas en el noreste de Tailandia. El estudio transversal descriptivo se aplicó para observar sistémicamente la glándula tiroides en caso de anomalía en 45 cadáveres embalsamados. El estudio se realizó en el Laboratorio de Anatomía de la Facultad de Medicina y Salud Pública de la Universidad de Ubon Ratchathani. Las anomalías de la glándula tiroides se registraron mediante fotografías antes de la clasificación de los datos y se utilizaron en un análisis descriptivo con SPSS. Los resultados mostraron que en 8 de los 45 cadáveres (17,8 %) se observaron anomalías de la glándula tiroides (4 hombres; 8,9 % y 4 mujeres; 8,9 %). Tales variaciones podrían ser clasificadas en 3 tipos: lóbulo piramidal (8,8 %), músculo elevador de la glándula tiroides (2,2 %) y la coexistencia del músculo elevador de la glándula tiroides (8,8 %), respectivamente. La anomalía de la glándula tiroides observada en este estudio se relacionó principalmente con informes anteriores. Además, la cantidad de variaciones tiroideas es una indicación crucial de la anomalía de la glándula tiroides que se repite, esta anomalía de la glándula es susceptible a las intervenciones de los médicos radiólogos en la tiroidectomía y la traqueotomía.

1-triacontanol cerotate isolated from Marsilea quadrifolia Linn. safeguards hippocampal CA3 neurons and augments special memory deficit in chronic epileptic rats / Cerotato de 1-triacontanol aislado de Marsilea quadrifolia Linn. protege las neuronas CA3 del hipocampo y mejora el déficit de memoria especial en ratas epilépticas crónicas

SUMMARY: Currently many people with epilepsy do not have seizure control even with the best available medications. Moreover various antiepileptics have adverse cognitive impact with other side effect. Thus, need for new antiepileptic drugs still remains challenge. However, many of the natural components have antiepileptic action and this fact remains scientifically unexplored. This study was designed to check the behavioral and neuro-pathological outcome of 1-Triacontanol cerotate (1TAC), isolated from Marsilea quadrifolia Linn. (MQ) on chronic Pentylenetetrazol (PTZ) kindling model of epilepsy in rats. Two-month-old adult male Wistar rats (n=60) were randomly divided into six groups; Group I (Cage Control), II (Vehicle Control), III (Positive Control), IV (Standard drug treated), V (1TAC: 40 mg/kg) & VI (1TAC: 80 mg/kg). To induce kindling a 35 mg/kg dose of PTZ was injected i.p. in every 48 hrs for 30 days in Group III to VI. Spatial memory performance was tested using Morris water maze, following which brains were further processed for histopathological investigations. Interestingly, 1TAC was able to minimize the loss of pyramidal cells in hippocampal CA3 region. These cellular changes were behaviorally responded as improved special learning and memory, a better spatial navigation and object place configuration. The current study strongly implicates that 1TAC from MQ has potent neuroprotective role and augments special memory deficit in chronic epileptic rats. The isolated component which attenuates spatial memory performance could be beneficial outcome to retain cognitive blunting in chronic epilepsy.

RESUMEN: Actualmente, muchas personas con epilepsia no cuentan con un control adecuado de las convulsiones, incluso con los mejores medicamentos disponibles. Además, varios antiepilépticos tienen un impacto cognitivo adverso además de efectos secundarios. Por lo tanto, la necesidad de nuevos fármacos antiepilépticos sigue siendo un desafío. Sin embargo, muchos de los componentes naturales tienen acción antiepiléptica y este hecho permanece científicamente inexplorado. Este estudio se diseñó para verificar el resultado conductual y neuro-patológico del cerotato de 1-triacontanol (1TAC), aislado de Marsilea quadrifolia Linn. (MQ) en el modelo de epilepsia en ratas del pentilenetetrazol (PTZ) crónico (PTZ). Ratas Wistar adultas de dos meses de edad (n = 60) se dividieron aleatoriamente en seis grupos; Grupo I (Control de jaula), II (Control de vehículo), III (Control positivo), IV (Medicamento estándar de tratamiento), V (1TAC: 40 mg / kg) y VI (1TAC: 80 mg / kg). Para inducir la inflamación se inyectó una dosis de 35 mg / kg de PTZ i.p. en cada 48 horas durante 30 días en los grupos III a VI. El rendimiento de la memoria espacial se probó utilizando el laberinto de agua de Morris, después de lo cual se procesaron los cerebros para investigaciones histopatológicas. Curiosamente, 1TAC pudo minimizar la pérdida de células piramidales en la región CA3 del hipocampo. Estos cambios celulares respondieron de manera conductual como una mejora del aprendizaje especial y la memoria, una mejor navegación espacial y la configuración del lugar del objeto. El estudio actual implica fuertemente que 1TAC de MQ tiene un potente papel neuroprotector y mejora el déficit de memoria especial en ratas epilépticas crónicas. El componente aislado que atenúa el rendimiento de la memoria espacial podría ser un resultado beneficioso para retener la reducción cognitiva en la epilepsia crónica.

Difference of brain-derived neurotrophic factor expression and pyramid cell count during mastication of food with varying hardness

Abstract Previous studies suggested that mastication activity can affect learning and memory function. However, most were focused on mastication impaired models by providing long-term soft diet. The effects of chewing food with various hardness, especially during the growth period, remain unknown. Objective: To analyze the difference of hippocampus function and morphology, as characterized by pyramidal cell count and BDNF expression in different mastication activities. Materials and Methods: 28-day old, post-weaned, male-Wistar rats were randomly divided into three groups (n=7); the first (K0) was fed a standard diet using pellets as the control, the second (K1) was fed soft food and the third (K2) was fed hard food. After eight weeks, the rats were decapitated, their brains were removed and placed on histological plates made to count the pyramid cells and quantify BDNF expression in the hippocampus. Data collected were compared using one-way ANOVA. Results: Results confirmed the pyramid cell count (K0=169.14±27.25; K1=130.14±29.32; K2=128.14±39.02) and BDNF expression (K0=85.27±19.78; K1=49.57±20.90; K2=36.86±28.97) of the K0 group to be significantly higher than that of K1 and K2 groups (p<0.05); no significant difference in the pyramidal cell count and BNDF expression was found between K1 and K2 groups (p>0.05). Conclusion: A standard diet leads to the optimum effect on hippocampus morphology. Food consistency must be appropriately suited to each development stage, in this case, hippocampus development in post-weaned period.

Ten-Year Follow-Up of Transcranial Magnetic Stimulation Study in a Patient With Congenital Mirror Movements: A Case Report

Most studies concerning congenital mirror movements (CMMs) have been focused on the motor organization in the distal hand muscles exclusively. To the best of our knowledge, there is no data on motor organization pattern of lower extremities, and a scarcity of data on the significance of forearm and arm muscles in CMMs. Here, we describe the case of a 19-year-old boy presenting mirror movements. In these terms, a 10-year transcranial magnetic stimulation study demonstrated that the motor organization pattern of the arm muscles was different from that of distal hand and forearm muscles even in the same upper extremity, and that the lower extremities showed the same pathways as healthy children. Moreover, in this case, an ipsilateral motor evoked potentials (MEPs) for distal hand muscles increased in amplitude with age, even though the intensity of mirror movements decreased. In the arm muscles, however, it was concluded that the contralateral MEPs increased in amplitude with age.

Morphological Variations of the Thyroid Gland in the North Indians: A Cadaveric Study with its Clinical Relevance

Introduction: The morphological variations of the thyroid gland are not an uncommon phenomenon and may due to embryological remnant or non-specific development of different parts of it. Prior anatomical knowledge of these variations is of immense importance to prevent catastrophies during or after thyroid surgeries. Therefore, in this study we aimed to investigate the prevalence of morphological variations of the thyroid glands in north Indian cadavers. Subjects and Methods: This study was conducted on 50 formalin embalmed adult human cadavers aging between 40–65 years, of which 40 were males and 10 were females. Thyroid glands were dissected and examined properly for the presence of pyramidal lobe, levator glandulae thyroidae, accessory thyroid tissue and complete absence of isthmus. Results: The pyramidal lobe was present in 9 (18%) and frequently arising from the right side of the isthmus. LGT was found in 7 (14%) and almost in all cases it was extending from the apex of the pyramidal lobe to the hyoid bone. Only 2 (4%) cadavers did not show an isthmus while accessory thyroid tissue was found only in one case. Morphological variations were more common in females than in males and the difference was statistically significant (p value<0.05). The means of all measured parameters were higher in female than in male but these gender differences were not significant (p>0.05). Conclusion: Morphological variation of the thyroid gland is a common phenomenon, particularly in female. Hence it requires proper detection and documentation prior to any thyroid surgery, so that iatrogenic catastrophies can be avoided

Age-dependent changes of p53 and p63 immunoreactivities in the mouse hippocampus / 한국실험동물학회지

P53 and its family member p63 play important roles in cellular senescence and organismal aging. In this study, p53 and p63 immunoreactivity were examined in the hippocampus of young, adult and aged mice by using immunohistochemistry. In addition, neuronal distribution and degeneration was examined by NeuN immunohistochemistry and fluoro-Jade B fluorescence staining. Strong p53 immunoreactivity was mainly expressed in pyramidal and granule cells of the hippocampus in young mice. p53 immunoreactivity in the pyramidal and granule cells was significantly reduced in the adult mice. In the aged mice, p53 immunoreactivity in the pyramidal and granule cells was more significantly decreased. p63 immunoreactivity was strong in the pyramidal and granule cells in the young mice. p63 immunoreactivity in these cells was apparently and gradually decreased with age, showing that p63 immunoreactivity in the aged granule cells was hardly shown. However, numbers of pyramidal neurons and granule cells were not significantly decreased in the aged mice with normal aging. Taken together, this study indicates that there are no degenerative neurons in the hippocampus during normal aging, showing that p53 and p63 immunoreactivity in hippocampal neurons was progressively reduced during normal aging, which might be closely related to the normal aging processes.

A Study of the Association between Psychotropic Drugs and Cognitive Dysfunction in Patients with Schizophrenia

Background: Cognitive deficits among patients with schizophrenia are now recognized as being widelyprevalent and one of the most disabling aspects of the illness, as they are associated with poor functionaloutcomes. Psychotropic medications and benzodiazepines, which are often used in these patients couldimpact cognition as could the extrapyramidal side-effects. Yet, here are hardly any Indian studies on thesubject. The aim of this study was to determine whether cognitive dysfunction among patients withschizophrenia is associated with extrapyramidal symptoms, anticholinergic burden of psychotropic drugsand benzodiazepine dosageMethodology: 40 clinically stable, out-patients suffering from schizophrenia, without any pre-existingneurological disorders between the ages of 18 to 60 years, who had been compliant with medications wereselected. Extrapyramidal symptoms (EPS) were assessed using the modified Simpson-Angus scale,anticholinergic burden (ACB) was evaluated using the Anticholinergic burden scale. The benzodiazepinedosage was noted. Cognition was assessed using the Stroop Color and Word Test and the Wisconsin Cardsorting test. The correlations between scores on EPS and ACB and neuropsychological tests were carriedout using partial correlations controlling for positive and negative symptoms.Results: There were no significant associations noted between extrapyramidal symptoms, anticholinergicburden, benzodiazepine dosage and performance on the neurocognitive tests used.Conclusion: Methodological differences make it difficult to construct comparisons across studies but thereis some evidence to support our findings

Hallazgo de quiste ependimario (ependimoma) intramedular en el estudio de la vía piramidal y síndrome siringomiélico / Finding of intramedullary ependymal cyst (Ependymoma) in the study of pyramidal tract and syringomyelic syndrome

Introducción: Una paresia tiene que ser objeto de un detallado análisis para conocer su correcta etiología. La exploración física debe incluir una minuciosa valoración neurológica que oriente la localización de la lesión. Caso Clínico: Paciente con paresia de extremidad inferior derecha, hiperreflexia, clonus y disminución de sensibilidad térmica y algésica por debajo de la apófisis xifoides. Estos hallazgos señalan a nivel vertebral dorsal, D8, posible localización de una lesión que afecta a la vía piramidal motora y la vía sensitiva. La sospecha clínica se confirma mediante RMN que mostró la presencia de masa intramedular, posible ependimoma. Conclusiones: El conocimiento de la anatomía de los haces nerviosos que discurren por la médula espinal y la información nerviosa que proporcionan, contienen signos clínicos motores y sensitivos que permiten discernir qué haces nerviosos están lesionados. Este juicio clínico puede dirigir, de manera mucho más concreta, hacia el tipo de exploraciones y pruebas complementarias que pueden ser necesarias para concretar un diagnóstico y reducir el gasto sanitario, así como el tiempo de espera para un posible tratamiento quirúrgico(AU)

Introduction: A paresis must be the object of a detailed analysis to know its correct etiology. Physical examination should include a thorough neurological assessment to guide the location of the lesion. Clinical case: Patient with paresis of the lower right extremity, hyperreflexia, clonus, and decreased thermal and algic sensitivity below the xiphoid process is preseneted. These findings point to the dorsal vertebral level, D8, possible location of a lesion that affects the pyramidal motor pathway and the sensory pathway. Clinical suspicion is confirmed by MRI that showed the presence of intramedullary mass, possible ependymoma. Conclusions: The knowledge of the anatomy of the nerve bundles that run through the spinal cord, and the nerve information they provide contain clinical and sensory clinical signs, allowing to discern which nerve bundles are injured. This clinical judgment can lead, in a much more concrete way, to the type of explorations and complementary tests that may be necessary to diagnose and reduce health costs, as well as the waiting time for a possible surgical treatment(AU)