RESUMO
Objective To investigate the process conditions for ?-cyclodextrin-inclusion of volatile oil obtained from Rhizoma Chuanxiong and Radix et Rhizoma Asari in Guanxin Zhitong Cataplasma,which is prescribed by Professor Deng Tietao.Methods The orthogonal design was used for the optimization of inclusion conditions.With the transformation rate of the volatile oil in inclusion complex and with the yield of inclusion complex as the evaluation indexes,the mass ratio of ?-cyclodextrin to oil,the temperature and the inclusion time were used as the main factors of L9(34)orthogonal design.Results The optimal conditions for inclusion were as follows: the mass ratio of ?-cyclodextrin to oil being 8∶1,in a saturated water solution,inclusion temperature at 60 ℃,and stirring for 1h.Conclusion The optimal conditions for ?-cyclodextrin-inclusion of volatile oil from Rhizoma Chuanxiong and Radix et Rhizoma Asari are steady and feasible.
RESUMO
Objective To determine the contents of free ferulic acid and total ferulic acid in different processed products of Rhizoma Chuanxiong.Methods With methanol-formic acid(95∶5) as the extraction solvent for free ferulic acid and with methanol-2% NaHCO3 water solution(95∶5) as the extraction solvent for total ferulic acid,ultrasonic method was used for the extraction.The contents of free ferulic acid and total ferulic acid were determined by HPLC,and the chromatographic conditions were as follows: C18 column(250mm?4.6mm,5?m),the mobile phase consisting of acetonitrile-1%acetic acid solution(20∶80),the detecting wavelength at 320nm,flow rate being 1.0 mL/min and detection at room temperature.Results The average contents of free ferulic acid and total ferulic acid in Rhizoma Chuanxiong roasted by wine were higher than those in raw and other processed Rhizoma Chuanxiong,and free ferulic acid content in raw and different processed Rhizoma Chuanxiong was lower than the total ferulic acid content.Conclusion The method is simple,accurate and can be used for the quality control standard for Rhizoma Chuanxiong,and the chemical assay of total ferulic acid content would be a better choice of assessing the herbal quality of Rhizoma Chuanxiong.