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Objective: To establish a cell culture model of cervical-loop epithelial cells from Wistar rat lower incisors on culture bottle coated with rat tail collagen. Methods: The effect of self-made rat tail collagen on the culture of cervical-loop epithelial cells was observed. The cells were identified by immunohistochemistry staining. Results: Cervical-loop epithelial cells in Wistar rat lower incisors grew well in self-made rat tail collagen. The cervical-loop epithelial cells exhibited positive expression for integrin-β1 and monoclonal antibody CK in immunohistochemistry staining. Conclusion: The cell culture model of cervical-loop epithelial cells in Wistar rat lower incisors with self-made rat tail collagen can be helpful to research tooth development mechanism.
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OBJECTIVE To establish a cell culture of human nasal epithelial cells on glass cover slides coated with rat tail collagen and explore a method to research human mucociliary system. METHODS The rat tail collagen and collagen coated cover slides were prepared for explant culture of human nasal epithelial cells. RESULTS The collagen on cover slides must be thin and ? at. It was about 1mm thick. The cultured epithelial cells were monolayer and polygonal cells. There was tight junction between the cells. Ciliary beat frequency of different cilia was equal on the same cell,and it was also equal of on cells from uncinate process and inferior turbinate. CONCLUSION This successful cell culture model of human nasal epithelail cell could be helpful to research the physiology and function of human nasal mucociliary system.
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Objective:To develop an experimental three-dimensional model by ECV304 cells(human umbilical vein endothelial cell line) for investigating the mechanisms of angiogenesis in vitro.Methods:ECV304 cells were seeded onto three-dimensional collagen gels made of rat-tail collagen.When the endothelial cells were cultured and grown to near confluence,treated with bFGF for 3 to 12 days,and then assessed with inverted phase contrast microscope.Results:The endothelial cells migrated into the gels,formed complex networks by cell cords at different levels through the bottom view,and sprouted capillary-like structures through the side view.Conclusion:ECV304 cells are capable of expressing some early events of angiogenesis in the three-dimensional collagen gels:proliferating,migrating and sprouting and so on.It should be useful for studying angiogenesis in vitro.