RESUMO
OBJECTIVE@#To investigate the correlation between soluble receptor for advanced glycation end products (sRAGE) level in the follicular fluid and ovarian responsiveness in non-PCOS patients undergoing controlled ovarian hyperstimulation.@*METHODS@#Ninety non-PCOS patients underwent IVF/ICSI using a short-acting long protocol for ovarian stimulation with a GnRH agonist. For each patient, the level of sRAGE in the follicular fluid was measured by enzyme linked immunosorbent assay (ELISA), and the data including the clinical baseline state, hormone level, number of oocytes obtained and the fertilization rate were collected.@*RESULTS@#Follicular fluid sRAGE level showed significant negative correlations with basal FSH level (=0.0036) and Gn dose ( 15) than in cases with oocytes obtained within the range of the target numbers (7-15) and below the target number (< 7) ( < 0.0001 and =0.0012, respectively).@*CONCLUSIONS@#Follicular fluid sRAGE level can reflect ovarian reserve function in non-PCOS patients, the number of oocytes obtained and the fertilization rate, and can thus predict ovarian responsiveness during controlled hyperstimulation in nonPCOS patients.
Assuntos
Feminino , Humanos , Fertilização in vitro , Líquido Folicular , Síndrome de Hiperestimulação Ovariana , Receptor para Produtos Finais de Glicação AvançadaRESUMO
Objective To investigate the protective effect of Inula britannica flower total flavonoids (IBFTF) on aging L929 cells induced by advanced glycation end products (AGEs), and to explore the mechanism of the expression of receptor advanced glycation end products (RAGE). Methods The senescence L929 cells model was established by using 0.100 g/L AGEs for 48 h culture in vitro, and then different concentration of IBFTF (0.1, 0.2, and 0.4 g/L) was given to the treatment group for 6 h culture, and 0.1 g/L aminoguanidine hemisulphate (AG) was given to the positive group for another 6 h culture, and while blank group was cultured with common culture medium. Senescence aging index of β-galactosidase staining cell numbers and cell cycle analysis in L929 cells were measured. The levels of reactive oxygen species associated with oxidative stress in the cells, and aging indicators such as superoxide dismutase (SOD), malondialdehyde (MDA) were also examined. The fluorescent intensity of RAGE was detected by immunofluorescence. The expression of RAGE protein and mRNA was detected by Western blotting and qPCR, respectively. Results IBFTF significantly inhibited AGEs-induced L929 cells senescence and decreased RAGE protein and mRNA expression. Different concentrations of IBFTF could significantly increase the SOD activity and reduce the MDA content and eliminate the reactive oxygen species in aging L929 cells in a dose-dependent manner. Conclusion Protective effect of IBFTF on aging L929 cells induced by AGEs may be related to its effect on the surpressing the expression of RAGE.
RESUMO
Objectives To explore the expression of the receptor for advanced glycation end products (RAGE) in pancreas and the serum concentration of RAGE in rats with acute necrotizing pancreatitis (ANP).Methods Sixty four male Spraque-Dawley rats were randomly divided into control group,ANP 6,18,24,36,48,72,96 h group with 8 rats in each group.The rat model of ANP was established by injecting 20％ L-arginine intraperitoneally at the dose of 250mg/100g body weight for twice at the interval of 1 h.Rats in control group were injected intraperitoneally with the same amount of saline.The pancreas samples were histologically examined by light microscope and scored.The amount of ascites,levels of serum amylase and RAGE was determined; Real-time PCR was performed to detect the expression of RAGE mRNA in pancreatic tissue.Results Pancreatic injuries aggravated with time.The amount of ascites increased from ( 1.98 ± 0.64) ml at 6h to (8.69 ±0.62)ml at 96 h.Serum amylase level began to increase at 6h after L-arginine intraperitoneal injection and reached the peak value at 18 h[(5069.88 ± 603.25 ) U/L],and returned to normal at 36 h.The serum concentration of RAGE and RAGE mRNA expression in pancreatic tissue were ( 18.33 ± 2.99) ng/ml and 0.41 ±0.13 in the control group.The corresponding values increased at 6 h in ANP group,which were (30.31 ±5.03) ng/ml and 1.57 ±0.19,and they were significantly higher than those in the control group (P ＜0.05) ; they reached the peak value at 24 h[( 105.41 ±21.31 ) ng/ml and 4.23 ±0.73],which were significantly higher than those in other ANP groups ( P ＜ 0.05 ) ; at 96 h they decreased to the lowest point [( 33.54 ± 6.96) ng/ml and 1.19 ± 0.19],which were still significantly higher than those in the control group (P ＜ 0.05 ).Conclusions The expression levels of RAGE in pancreatic tissues and serum level of RAGE increase within 36 h of ANP onset,then decrease gradually,but they are always higher than normal values.