Effect of laboratory referencing on data analysis of community viral load in HIV positive MSM from 15 cities, China / 中华流行病学杂志

Objective To compare the community viral load (CVL) among MSM in 15 cities in China using standardized national reference sources.Methods The study analyzed the existing database of National Major Science and Technology Project of China.The database was established with serial random survey of MSM HIV CVL among MSM in 15 cities from 2013 to 2015.VL tests were conducted in 15 laboratories with different equipment and methods,including RT-PCR,nucleic acid sequence based amplification (NASBA),branched DNA testing (bDNA) and Abbott M2000 RealTime system (M2000).Based on proficiency test for 15 laboratories conducted by National HIV Reference Laboratory,VL test values detected with EasyQ,bDNA and M2000 were converted and standardized into resultant values of TaqMan 2.0.Software SPSS 17.0 was used to produce descriptive statistics for the dataset.Results From 2014 to 2015,the 15 testing sites were found to use a number of different viral load detection techniques.In 2014,the community viral load values were (2.38 ±1.47) and (2.99 ± 1.31) in 15 testing sites,while in 2015 these values were found to be (2.07± 1.34) and (2.72± 1.19).The measurement of community VL was done using standard benchmarks of ≤200 copies/ml,≤400 copies/ml and ≤1 000 copies/ml,that were used for reference for now.Conclusion It is necessary to use standard detection method to improve the comparability of annual results.Using a standardized rate of ≤400 copies/ml or ≤ 1 000 copies/ml for successful control of VL was found with high stability for the result comparison among different areas.

Effect of laboratory referencing on data analysis of community viral load in HIV positive MSM from 15 cities, China / 中华流行病学杂志

Objective To compare the community viral load (CVL) among MSM in 15 cities in China using standardized national reference sources.Methods The study analyzed the existing database of National Major Science and Technology Project of China.The database was established with serial random survey of MSM HIV CVL among MSM in 15 cities from 2013 to 2015.VL tests were conducted in 15 laboratories with different equipment and methods,including RT-PCR,nucleic acid sequence based amplification (NASBA),branched DNA testing (bDNA) and Abbott M2000 RealTime system (M2000).Based on proficiency test for 15 laboratories conducted by National HIV Reference Laboratory,VL test values detected with EasyQ,bDNA and M2000 were converted and standardized into resultant values of TaqMan 2.0.Software SPSS 17.0 was used to produce descriptive statistics for the dataset.Results From 2014 to 2015,the 15 testing sites were found to use a number of different viral load detection techniques.In 2014,the community viral load values were (2.38 ±1.47) and (2.99 ± 1.31) in 15 testing sites,while in 2015 these values were found to be (2.07± 1.34) and (2.72± 1.19).The measurement of community VL was done using standard benchmarks of ≤200 copies/ml,≤400 copies/ml and ≤1 000 copies/ml,that were used for reference for now.Conclusion It is necessary to use standard detection method to improve the comparability of annual results.Using a standardized rate of ≤400 copies/ml or ≤ 1 000 copies/ml for successful control of VL was found with high stability for the result comparison among different areas.

Referencing and inosculating--discussion on modern research strategy for ethnomedicines / 中国中药杂志

This article reviewed the current situation of ethnomedicines in China, and propos that the important research on ethnomedicine is to focus on the theoretical and empirical characteristics of ethnomedicine and the special features in the processes of collecting,processing and clinical application of ethnomedicine, which will be benefit to find the research principles and methods with the characteristics of ethnomedicine. These principles include making clear of the effective substances and mechanisms, and theoretical and empirical characteristics of ethnomedicine. The three methods in the new drug discovery technology system for ethnomedicine are to solve the difference in varieties of traditional medical systems depending on finding the similarities of them, to find the conjunction in varieties of traditional medical systems through the key-herbs, and to find medicinal sources in varieties of traditional medical systems according to pharmaphylogeny.

An Ontology-Based GIS for Genomic Data Management of Rumen Microbes

During recent years, there has been exponential growth in biological information. With the emergence of large datasets in biology, life scientists are encountering bottlenecks in handling the biological data. This study presents an integrated geographic information system (GIS)-ontology application for handling microbial genome data. The application uses a linear referencing technique as one of the GIS functionalities to represent genes as linear events on the genome layer, where users can define/change the attributes of genes in an event table and interactively see the gene events on a genome layer. Our application adopted ontology to portray and store genomic data in a semantic framework, which facilitates data-sharing among biology domains, applications, and experts. The application was developed in two steps. In the first step, the genome annotated data were prepared and stored in a MySQL database. The second step involved the connection of the database to both ArcGIS and Protege as the GIS engine and ontology platform, respectively. We have designed this application specifically to manage the genome-annotated data of rumen microbial populations. Such a GIS-ontology application offers powerful capabilities for visualizing, managing, reusing, sharing, and querying genome-related data.

Agricultural exploitation impact on the quality of natural waters in the trout-breeding region of the Mantiqueira ecological corridor / Impacto da exploração agropecuária na qualidade de águas naturais em região de truticultura no corredor ecológico da Mantiqueira

Seasonal assessment of water quality was undertaken in an agricultural cattle-raising and trout-breeding region. Seventeen sites were sampled in ten municipalities close to the Papagaio and Ibitipoca State Parks in Minas Gerais, Brazil. Landsat 5 satellite images and Spotmap mosaic images with resolution of 2.5 m were employed to correlate classes of land use and topographic characteristics with the environmental contamination index of the water bodies at these sites. Results show a change in hydric classification of three sites evaluated. During the rainy season, sites 3, 7 and 17 were classified as class 3 according to CONAMA regulations, whereas the other sites were classified as class 2. During the dry season, sites 10 and 11 belonged to class 3 and the others to class 2. Literature and analysis of images and visits on the area showed that the principles of sustainable farming were not being followed in the region. Current research may be a tool for planning the preservation of natural resources. Results indicate there is a need for specific planning for fish farming and agriculture in the region for its sustainable economic exploitation.

Realizou-se a avaliação sazonal da qualidade da água, em região de truticultura e agropecuária, por amostragem, em 17 pontos, dez municípios, em torno dos parques estaduais do Papagaio e do Ibitipoca em Minas Gerais, Brasil. Usando imagens gratuitas Landsat 5 e imagens de mosaico Spotmap, resolução de 2,5 m, correlacionando as classes de uso da terra e características topográficas com o índice de contaminação ambiental dos corpos d'água nesses pontos. Os resultados mostram alteração da classificação hídrica de três dos pontos avaliados. Na época das chuvas, os pontos 3, 7 e 17 foram classificados como classe 3, de acordo com as normas do Conama (Conselho Nacional do Meio Ambiente), enquanto os outros pontos foram 2. Durante a estação seca, os pontos 10 e 11 eram de classe 3 e os outros como classe 2. A revisão de literatura, com a análise das imagens e visitas na área, mostrou que os princípios da agricultura sustentável não estão sendo seguidos na área. Este trabalho serve como ferramenta para o planejamento para preservação dos recursos naturais. Os resultados indicam que há a necessidade de um planejamento específico para a criação de peixes e agropecuária na região, permitindo assim sua exploração sustentável.