Rheum officinale at different years old stage / 中草药

Objective To analyze the content and variation rules of 10 constituents in radix, rhizome, and leaf of Rheum officinale at one-, two-, and three-year-old stage, respectively, and provide theoretical guidance for efficient production and quality control of the crud drug. Methods The content of each constituent in R. officinale was determined by high performance liquid chromatography (HPLC), and one factor analysis of variance and multiple comparison were performed by SPSS 24.0. Results HPLC system was established for the determination of 10 components in R. officinale. The linear range was good (r2 > 0.997), RSD of precision, stability, and repeatability were less than 2%, and the recoveries were 96.10%—107.10%, respectively. The content analyses showed that, in the same part, the content of gallic acid decreased significantly year by year or at the 2nd growth years (P 3 > 2 (P 1 > 2 (P radix > leaf (P < 0.05). Conclusion The HPLC based determination of 10 constituents in R. officinale showed that the accumulation profiles of the samples at different years or from different parts varied. For the same parts, the contents of most constituents increased year by year. During the same growth year, the contents of most constituents in radix or rhizome were higher than those in leaf. The radix and rhizome of the three years old samples had the highest contents of main constituents.

Effect of Rheum officinale on activity of intestinal stem cells in Drosophila melanogaster induced by SDS toxicity / 中草药

Objective: To explore the effect of Rheum officinale extracts (ROE) on activity of intestinal stem cells of Drosophila melanogaster. Methods: The control group was fed with normal cornmeal-yeast medium, and the experimental group was fed with cornmeal-yeast medium containing 0.05 or 0.1 g/mL ROE. In the experiment, the gut damage was induced by feeding D. melanogaster with toxic compounds. The effects of ROE on survival rate, number and morphology of progenitor cells, proliferation and differentiation of intestinal stem cells, expression of reactive oxygen species (ROS), number of intestinal epithelial dead cells and life span of D. melanogaster were detected and analyzed. Results: ROE (0.05 and 0.1 g/mL) could increase the survival rate of D. melanogaster induced by toxic compounds. ROE (0.1 g/mL) could decrease SDS-induced ROS levels, reduce the number of intestinal epithelial dead cells, inhibit excessive proliferation and differentiation of intestinal stem cell, alleviate the excessive accumulation of progenitor cells, thereby maintain homeostasis in the gut. In addition, ROE could prolong the lifespan of D. melanogaster. Conclusion: ROE can inhibit excessive proliferation and differentiation of intestinal stem cells, enhance gut immune function, and prolong the life span of D. melanogaster.

Quality control for extracts from aerial parts of Rheum officinale based on multi-assay depending on one determination / 中草药

Objective: To establish a method for the quality control of the extracts from the aerial parts of Rheum officinale (EARO) based on multi-assay depending on one determination (MDOD). Methods: The quality control of EARO was carried out through the thin-layer chromatography(TLC), discrimination of chromatographic peak in fingerprint, ultraviolet spectrophotometric, and HPLC methods. The relative correction factors of aloe-emodin, chrysophanol, and physcione for detecting emodin were set up, the factors were used to measure their contents, and the method was adopted to the quality control of EARO. Results: The experimental and control samples in TLC showed the same colored spots at the same position in EARO, five characteristic peaks among six were identified, and showed a good linear relationship at the range of 4.944-29.664 μg/mL (r = 0.9990). The method of MDOD showed the recoveries of aloe-emodin, emodin, chrysophanol, and physcione were 97.08%, 95.78%, 100.60%, and 97.47%, and the RSD values were 3.32%, 2.42%, 3.72%, and 2.67%. There were no significant differences between MDOD method and external standard method. Conclusion: TLC and fingerprint could be used to identify the chemical constituents in EARO, and ultraviolet spectrophotometry could be used to control the quantity of the total anthraquinone. The method of MDOD could be used to quantitatively control the contents of aloe-emodin, emodin, chrysophanol, and physcione, and the method is simple and accurate, which has a higher repeatability.

Extraction optimization of anthraquinone from aerial part of Rheum officinale Baill based on Plackett-Burman design combined with CCD response surface methodology / 中国药学杂志

OBJECTIVE: To optimize the process of extacting effective constituents from aerial part of Rheum officinale Baill by Plackett-Burman design combined with CCD response surface methodology. METHODS: In Plackett-Burman combined with CCD response surface design, the independent variables were concentration of ethanol, extraction time, and solvent fold. The dependent variables were contents of total anthraquinone and rheum emodin. RESULTS: The optimal extraction process of total anthraquinone was as follows: 67.25% ethanol, 90 min for reflux per time, 28:1 fold of solvent and 2 times of extraction; the optimal extraction process of emodin was 85% ethanol, 90 min for reflux per time, 40 fold of solvent and 2 times of extration. CONCLUSION: The optimal process is simpleand convenient for extracting aerial part of Rheum officinale Baill with high precision and predictability. Copyright 2012 by the Chinese Pharmaceutical Association.