Progress toward the clinical application of Rhodiolae Crenulatae Radix et Rhizoma / 国际中医中药杂志

Rhodiolae Crenulatae Radix et Rhizoma is a precious traditional Chinese medicine and has extensive pharmacological effects, such as anti-hypoxic, anti-fatigue, anti-aging, anti-inflammatory, anti-tumor, lowing blood sugar, heart and brain protection. It has been applied to the treatment of cardiovascular diseases, nervous diseases, respiratory diseases, endocrine diseases, fracture problems, emotional stress, and skin diseases. The present review summarized the literature about its clinical use, which would provide a reference for further utilization.

Determination of six flavonoids in Rhodiolae Crenulatae Radix et Rhizoma by HPLC-DAD / 中草药

Objective: To establish a determination method for the contents of ternatumoside II, rhodiosin, rhodionin, herbacetin, kaempferol, and rhodiolin in Rhodiolae Crenulatae Radix et Rhizoma and to compare the content differences of the six flavonid compounds in Rhodiolae Crenulatae Radix et Rhizoma from different sources. Methods: Using HPLC-DAD method and Kromasil C18 column (250 mm × 4.6 mm, 5 μm), with acetonitrile and water as mobile phase, gradient elution at flow rate of 1.0 mL/min and column temperature of 35℃. The detection wavelength was 382 nm. Results: Ternatumoside II, rhodiosin, rhodionin, herbacetin, kaempferol, and rhodiolin had good linearity in the ranges of 0.015 08-0.301 6, 0.123 2-2.464, 0.109 5-2.190 8, 0.007 8-0.156, 0.021 46-0.429 2, and 0.006 48-0.129 6 μg, respectively. The average recoveries of the six constituents were 98.17%-100.52% and RSD values were 1.64%-1.98%. Conclusion: The method is simple and accutate. It provides the reference for comprehensive quality control of Rhodiolae Crenulatae Radix et Rhizoma.

Ultrasonic Extraction Process Research and Mathematic Simulation of Tyrosol in Rhodiolae Crenulatae Radix et Rhizoma / 中国中医药信息杂志

Objective To study the ultrasonic extraction technology of tyrosol in Rhodiolae Crenulatae Radix et Rhizoma;To mathematically simulate the extraction process.Methods The content of tyrosol was set as index;HPLC was used;the ultrasonic extraction process of tyrosol in Rhodiolae Crenulatae Radix et Rhizoma was optimized by an orhogonal experiment and mathematic simulation.Results The optimum ultrasonic extraction conditions were as follows:80%ethanol was used as extraction solvent, the ratio of material to liquid was 1:35 (g:mL), with the extraction time of 30 min and ultrasonic power of 240 W. In these conditions, the extracting rate of tyrosol was 0.150 1%. Compared with the heating reflux method, the extraction time should be shortened by 66.7% and the extracting rate should be increased by 12%.Conclusion The extraction method is simple and the extraction rate of effective components is high. Mathematical simulation values based on ultrasonic extraction are consistent with experiment values.

Purification technology of salidroside by microporous adsorption resin / 中草药

Objective: To optimize the purification technology of salidroside with macroporous adsorption resin. Methods: The absorption and elution conditions of salidroside purificatin by various macroporous adsorption resins were investigated with the content of salidroside as index. Results: SP-825 type macroporous adsorption resin was the best choice for the purification of salidroside. The optimized parameters were as follows: the content of salidroside at 1.713-2.570 g/mL, eluting solvent of 10% aqueous ethanol, volume flow at 2 BV/h, and eluant volume at 30 BV. Conclusion: SP-825 type macroporous adsorption resin could significantly increase the purity of salidroside with the advantage of high absorption, high elution rate, low cost, environment friendly, and being applicable for the production in large scale.