Content determination of five flavonoids in Tibetan medicine Rhododendron anthopogonoides by quantitative analysis of multi-components by single marker (QAMS) / 中国中药杂志

To establish a quantitative analysis of multi-components by single marker(QAMS) method for five flavonoids in Rhododendron anthopogonoides and verify its feasibility and applicability in the medicinal materials of R. anthopogonoides. With hyperoside as the internal reference, relative correction factors(RCF) of rutin, quercetin, quercitrin and kaempferol were established by high-performance liquid chromatography(HPLC) analysis. RCFs were used to calculate the content of each component, system durability and relative retention time. Simultaneously, QAMS and external standard method(ESM) were used to determine the content of five flavonoids in 12 batches of R. anthopogonoides from different origins. The results were statistically analyzed to verify the accuracy and feasibility. The fingerprints and cluster analysis data of R. anthopogonoides analyzed and discussed differences among the batches. According to the results, the RCFs of rutin, quercetin, quercetin and kaempferol in R. anthopogonoides were 1.242 6, 0.990 5, 0.535 0, and 0.781 3, respectively. The RCFs represented a good reproducibility under different experimental conditions. Besides, there was no significant difference between QAMS and ESM. Besides, the fingerprint and cluster analysis data showed the consistency between the classification and with the origin distribution of the herbs. In conclusion, the QAMS method shows a good stability and accuracy in the quality control of R. anthopogonoides.

Chemical constituents from Rhododendron anthopogonoides / 中草药

Objective: To study the chemical compositions of Rhododendron anthopogonoides. Methods: The chemical constituents were isolated from R. anthopogonoides by Sephadex LH-20, MCI, silica gel column chromatography, and preparative-HPLC. The structures were assigned by physicochemical property and spectroscopic data. Results: Ten compounds have been isolated from R. anthopogonoides and their structures were indentified as caffeic acid (1), trans-ferulic acid (2), kaempferol (3), dihydroquercetin (4), noreugenin (5), 4-(3,4-dihydroxyphenyl)-2-butanone (6), 3,5-dihydroxy toluene (7), ursolic acid (8), ethyl 3,4-dihydroxybenzoate (9), and procurcumenol (10). Conclusion: Compounds 1-3, 5-7, 9, and 10 are obtained from R. anthopogonoides for the first time. Compounds 2,6,9, and 10 are obtained from the plants of Rhododendron L. for the first time.

Inclusion complexes of azalea oil with ?-cyclodextrin / 中成药

Objective: To study the inclusion effect and rules of azalea oil (rhododendron anthopogonoides marxim) and ? cyclodextrin(? CYD) in different proportion. Methods: An optimal condition for the preparation of the azalea oil ? CYD was established by orthogonal test. Results: The optimal condition was that stiring, 30 ?C , 30min, ? CYD:oil=8g∶1mL Conclusion: This optimized process is simple, stable and efficient.