RESUMO
Objective: To promote the comprehensive utilization in the root barks and stem barks of Toddalia asiatica and to establish an HPLC method for the determination of the fingerprints for the root barks and stem barks of T. asiatica. Meanwhile, the similarity was compared by similarity evaluation software based on the fingerprints. Methods: HPLC analysis was performed on C18 (250 mm × 4.6 mm, 5 μm) with acetonitrile and 0.1% aqueous solution of phosphoric acid as mobile phase in gradient mode at the wavelength of 269 nm, and the flow rate was 1 mL/min. The similarity was calculated by comparison on the fingerprints derived from 10 bathes of T. asiatica root barks and 10 bathes of T. asiatica stem barks, all the samples were collected from the different areas in different seasons. Results: The mutualmode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different sources were compared. The content ratio of active ingredients is different from samples in different areas and seasons. Conclusion: The fingerprint similarity is greater than 0.9 between T. asiatica root barks and T. asiatica stem barks, so the compositions are highly similar.
RESUMO
Objective: To study the chemical constituents from the root barks of Lycium chinense. Methods: The chemical constituents of EtOAc fraction from 95% ethanol extract of L. chinense were isolated and purified by chromatography on silica gel, Sephadex LH-20, and ODS. Their chemical structures were identified on the basis of physicochemical properties and spectroscopic data. Results: Twelve compounds were isolated and identified as N-trans-coumaroyltyramine (1), N-trans-feruloyltyramine (2), dihydro-N-caffeoyltyramine (3), apigenin (4), ferulic acid (5), p-hydroxycinnamic acid (6), 3-hydroxy-1-(4-hydroxyphenyl)-1-propanone (7), 3, 4-dihydroxybenzenepropionic acid (8), 3, 4-dihydroxybenzenepropionic acid methyl ester (9), p-hydroxy-benzoic acid (10), 4-methoxy salicylic acid (11), and nicotinic acid (12). Conclusion: Compounds 8 and 9 are two new natural products, and compounds 1, 6, 11, and 12 are obtained from this plant for the first time.
RESUMO
Objective: To study the chemical constituents from the root barks of Ginkgo biloba. Methods: The constituents were isolated by column chromatography and their structures were elucidated by physicochemical properties and spectroscopic analyses. Results: Thirteen compounds were isolated including four fatty acids, palmitinic acid (1), stearic acid (2), behenic acid (3), and lignoceric acid (4); one fatty alcohol, 1-heptacosanol (5); two sterides, β-sitosterol (6) and daucosterol (7); two flavonoids, genkwanin (8) and apigenin (9); and four ginkgolides, bilobalide (10), ginkgolide A (11), ginkgolide B (12), and ginkgolide C (13). Conclusion: Among them, compounds 3-5 are isolated from this plant for the first time. The results are helpful for the comprehensive utilization of the resources of the root barks of G. biloba.