Effects of Uygur Medicine Branchlets roses extracts on blood glucose and glucose tolerance in diabetes mice induced by alloxan / 药物评价研究

Objective To observe the effect of extract from Branchlets roses on blood glucose and glucose tolerance in diabetes mice induced by alloxan.Methods Diabetes animal model was established by alloxan.Dividing the model mice into eight groups:model group,water extract high,middle,and low dose (3.70,1.85,and 0.93 g/kg) group,and ethanol extract high,middle,and low dose (2.75,1.37,and 0.70 g/kg) group,and metformin (positive drug,200 mg/kg) group,and normal mice were taken as control group.Drug was ig administered to mice 3 d after molding once daily.Blood glucose test paper was used to determine fasting blood glucose 0,10,20,and 28 d after modeling,and the glucose tolerance test was performed 30 d after modeling.Results The extract of Branchlets roses from all the groups could decrease the blood glucose and improve the glucose tolerance,and showed a certain dose-effect relationship.In all the extracts,the alcohol extract had the best effect,but the effect was not as good as the positive control drug metformin hydrochloride group.Conclusion The extract of Branchlets roses can reduce the blood sugar content of diabetic mice,and improve the glucose tolerance.

ISSR analysis and identification of medicinal plants Rosa chinensis, Rosa rugosa, and their relative species / 中草药

Objective: To study the genetic diversity and genetic relationship of medicinal plants Rosa chinensis, R. rugosa, and their relative species by ISSR molecular marker technique, and provide the reference for Rosa L. germplasm identification and breeding. Methods: Fifteen species (including 33 samples) of medicinal herbs R. chinensis, R. rugosa, and their relative species were studied by ISSR-PCR markers. Nei's genetic diversity index (H) and other parameters of genetic information were calculated by POPGEN 32, and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean (UPGMA) by NTSYS-pc software. Results: Six ISSR primers generated 110 loci of which 109 loci were polymorphic. The average percentage of polymorphie bands (PPB) was 99.09%. Nei's genetic diversity index (H) and Shannon's information index (I) were 0.370 5 and 0.546 4, and the coefficient of genetic differentiation (Gst) and gene flow (Nm) were 0.886 8 and 0.063 8 between the species levels. The genetic distance (D) varied from 0.169 1 to 0.730 2. In the cluster dendrogram, 15 species were clustered into six groups at the level of Genetic similarity coefficient (GS) 0.60. Conclusion: The results of ISSR analysis reveal that medicinal plants R. chinensis, R. rugosa and their relative species had the plentiful genetic diversity and the genetic relationships were consistent with morphological characters of taxonomy. ISSR method is efficient for identification of R. chinensis, R. rugosa, and their relative species, which could provide a scientific basis for the resource collection and identification of the species in Rosa L.

Anti-Inflammatory Effect of Rosa rugosa Flower Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages

Rosa rugosa Thunb, a deciduous shrub of the genus Rosa, has been widely used to treat stomach aches, diarrhoea, pain, and chronic inflammatory disease in eastern Asia. In recent years, our research team has extensively studied the Rosa rugosa flower extract, and specifically undertook pharmacological experiments which have optimized the extraction process. Our methods have yielded a standard extract enriched in phenolic compounds, named PRE. Herein, we expand our efforts and evaluated the anti-inflammatory activity of PRE on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages. PRE significantly inhibited production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor (TNF)-a, interleukin (IL)-6, and interleukin 1β (IL-1β), as well as expression of their synthesizing enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase2 (COX-2). Furthermore, PRE inhibited activity of mitogen-activated protein kinases (MAPK) as well as nuclear factor-kappa B (NF-κB) signaling pathway. Our findings are the first to explain the anti-inflammatory mechanism by PRE in LPS-stimulated macrophages. Given these results, we propose that PRE has therapeutic potential in the prevention of inflammatory disorders.

Isoflavones from flower buds of Rosa rugosa and their cytotoxicity / 中草药

Objective: To study the chemical constituents from the flower buds of Rosa rugosa. Methods: The chemical constituents from the flower buds of R. rugosa were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatography and high performance liquid chromatography (HPLC) methods. Their structures were elucidated by spectroscopic methods and physicochemical properties. Results: Three isoflavones, 6,8-dihydroxy-4',7-dimethoxyisoflavone (1), prunetin (2), and pratensein (3) were isolated from the flower buds of R. rugosa. Conclusion: Compound 1 is a new compound named rosa isoflavone. Compounds 2 and 3 are isolated from R. rugosa for the first time. Compound 1 displays the stronger cytotoxicity against A549 and PC3 cells with IC50 values of 2.6 and 3.2 μmol/L, respectively.

Flavonoids from flower buds of Rosa rugosa / 中草药

Objective: To identificate the bioactive natural products, and study the chemical constituents in the flower buds of Rosa rugosa. Methods: The chemical constituents in the flower buds of R. rugosa were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatography, and high performance liquid chromatography (HPLC) methods. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Results: Nine flavonoids were isolated from the flower buds of R. rugosa, and identified as 8-acetyl-4',7-dimethoxy-6-methyl-flavone (1), kaempferol (2), kaempferol-3-O-β-D-glucopyranoside (3), kaempferol-3-O-β-D-rutinside (4), quercetin (5), quercetin-3-O-β-D-glucopyranoside (6), rutin (7), luteolin (8), and luteolin-7-O-β-D-glucopyranoside (9). Conclusion: Compound 1 is a new compound and it displays the cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 6.8, 5.6, 2.2, 1.8, and 7.4 μmol/L, respectively. Compound 9 is isolated from R. rugosa for the first time.

ANALYSIS OF NUTRITION COMPONENTS IN THE FRUITS OF WILD ROSA RUGOSA THUNB / 营养学报

This paper deals with the nutrition components analysis on the fruits of wild Rosa rugosa Thunb. The results showed that total sugar was 8.21%, total acid 1.08%, protein 0.89%, pectin 1.43%, tannin 1.63%, vitamin C 1857-2027 mg/100g. The contents of 16 kinds of inorganic components and 16 kinds of amino acids were assayed too.