Bactericidal Effect of Selenium Nanoparticles Combined with Povidone-iodine on Pathogenic Bacteria in Surgical Site Infection / 中山大学学报(医学科学版)

ObjectiveTo investigate the bactericidal effect of loaded multifunctional povidoneiodine-nanometer selenium （PVP-I@Se） disinfectant on Staphylococcus aureus （SA） and methicillin-resistant Staphylococcus aureus （MRSA）， and to provide an experimental basis for the reduction of surgical site infection （SSI）. MethodsThe control group was the povidone iodine （PVP-I） group with different concentrations of iodine （50， 75， 100， 200 and 400 μg/mL）. The PVP-I@Se group （experimental group） was the PVP-I group further supplemented with 2 μg/mL Selenium nanoparticles （SeNPs）. Then we compared the bactericidal effect of the two groups of disinfectant solutions on SA and MRSA by examining the minimum inhibitory concentration （MIC）， minimum bactericidal concentration （MBC）， the shortest sterilization time at a concentration of 50 μg/mL iodine and the inhibition zone diameters at concentrations of 200 μg/mL and 400 μg/mL iodine. ResultsMIC values of PVP-I against SA and MRSA were both 79.17 μg/mL， and those of PVP-I@Se were 54.17 and 70.83 μg/mL， respectively. MBC values of PVP-I against SA and MRSA were 129.17 and 150.00 μg/mL， respectively， and those of PVP-I@Se were 70.83 and 87.50 μg/mL， respectively. At a concentration of 50 μg/mL iodine， the shortest sterilization time of PVP-I for SA and MRSA was 130 s and 140 s， respectively， and that of PVP-I@Se was 65 s and 75 s， respectively. At a concentration of 200 μg/ml iodine， the inhibition zone diameters of PVP-I for SA and MRSA were 7.67 mm and 8.33 mm， and those of PVP-I@Se were both 9.50 mm. At a concentration of 400 μg/mL iodine， the inhibition zone diameters of PVP-I for SA and MRSA were 9.00 mm and 9.33 mm， and those of PVP-I@Se were 11.67 mm and 12.00 mm， respectively. ConclusionsPVP-I with different concentrations of 50， 75， 100， 200 and 400 μg/mL iodine supplemented with 2 μg/mL SeNPs have better and faster bactericidal effect on SA and MRSA. When combined with SeNPs， PVP-I can enhance the bactericidal activity against SA and MRSA， but with better sensitizing effect on SA than MRSA and higher demand of iodine concentration （400 μg/mL） for sensitizing effect on MRSA. This study provides a theoretical basis for selecting optimal concentration and action time of the disinfectant， thus reducing SSI.

Clinical efficacy of pemetrexed disodium in maintenance treatment of advanced lung adenocarcinoma / 中国基层医药

Objective:To investigate the clinical efficacy and adverse reactions of pemetrexed disodium in the maintenance treatment of advanced lung adenocarcinoma after chemotherapy with pemetrexed disodium and platinum.Methods:The clinical data of 35 patients with stage Ⅳ lung adenocarcinoma who received chemotherapy with pemetrexed disodium and platinum and were well treated in Beijing Huairou Hospital from January 2013 to August 2020 were retrospectively analyzed. Maintenance therapy with pemetrexed disodium was initiated after the completion of combination chemotherapy until disease progression. The clinical characteristics, therapeutic effects, adverse reactions, progression-free survival, and overall survival of the 35 patients were evaluated.Results:Among the 35 patients, no patients had complete remission, 11 patients had partial remission, 22 patients had stable disease, and 2 patients had progressive disease. The objective remission rate was 31.4%, disease control rate was 94.3%, median progression-free survival was 9.53 months, median overall survival was 18.21 months, 1-year survival rate was 68.6%, 2-year survival rate was 31.4%, and 3-year survival rate was 11.4%. Gender, age, smoking, and the baseline characteristics of patients undergoing first-line pemetrexed disodium or second-line pemetrexed disodium treatment had no effects on progression-free survival (all P > 0.05). Positive gene mutation and receiving four or more chemotherapy cycles had a protective effect on progression-free survival (both P < 0.05). Chemotherapy-related adverse reactions mainly included myelosuppression, nausea, elevated transaminase, and nephrotoxicity, all of which were mild and were relieved after symptomatic treatment. Conclusion:Pemetrexed disodium is effective and safe in the maintenance treatment of advanced lung adenocarcinoma. The results of this study are scientific.

Preparation and Performance of Three-Dimensional Silk FibroinScaffolds with Different Matrix Stiffness / 医用生物力学

Objective After hydrogen bonding between collagen ( COL) and silk fibroin ( SF ) at different concentrations, a composite scaffold with adjustable stiffness was prepared by combining with gel system, and its physical and chemical properties were characterized. Methods SF with different qualities was dissolved in sodium alginate (SA) solution, then COL solution at different concentration and calcium carbonate ( CaCO3 ) powder were added. The hydrogels of SC1, SC2, and SC3 groups were obtained by taking out the mixed solution and adding some gluconic acid lactone ( GDL) powder, and different SF scaffolds were obtained after freeze drying. Results The SF scaffolds with adjustable stiffness were successfully prepared. The compression moduli of SC1, SC2, and SC3 groups were (17. 31±2. 73), (24. 12±1. 81), (32. 54±1. 81) kPa, respectively. The innerstructure of the scaffolds was observed. From SC1 group to SC3 group, pores of the scaffolds were smaller and fewer, and hydrophilicity of the materials become better and better. Conclusions Three-dimensional ( 3D) porous scaffolds with different matrix stiffness can be prepared by changing the concentration of SF and COL solution. The concentration of SF and COL is proportional to the compression modulus, water absorption, water retention and swelling rate of SF scaffolds, while inversely proportional to porosity. The findings of this study are expected to provide theoretical guidance for construction of scaffolds with appropriate matrix stiffness for inducing osteogenic differentiation of mesenchymal stem cells

ACUTE ENCEPHALITIS SYNDROME IN A TERTIARY CARE SETTING WITH SPECIAL REFERENCE TO JAPANESE ENCEPHALITIS: AN OBSERVATIONAL STUDY IN ASSAM, INDIA

Acute Encephalitis Syndrome (AES) is a major public health problem and Japanese Encephalitis (JE) is one of the most important causes of AES. Therefore, it is crucial to know the etiology of AES for patient management and decision making. The present study aimed to assess the morbidity and mortality profile of AES/JE patients admitted in tertiary care setting. An observational prospective cross-sectional study was conducted among 140 hospitalized AES patients admitted in Pediatric and Medicine ward of Jorhat Medical College and Hospital (JMCH), Jorhat over a period from August, 2017 to July 2018. Blood serum and cerebrospinal fiuids (CSF) were tested for presence of JE specific IgM antibody by Mac ELISA during acute illness of AES. The data were compiled and analyzed using the IBM SPSS, V23.0. Of the total 140 AES cases, majority (60%) were below 15 years age (p-value 0.180). Significantly higher proportion of AES cases were from rural areas (94.3%) (p-value <0.0001). The male and female ratio was 1.3:1. The most common presenting symptoms in AES patients were fever (100%), change in mental status (100%), seizure (56.4%) and headache (42.9%). Signs of meningeal irritation were present in 69.3% of cases. Around 22.9% AES patients had GCS ? 8. A total 49 (35%) cases were found to be laboratory confirmed JE following detection of JE specific IgM antibody. Among the JE patients, only 4.1% were immunized with SA 14-14-2 vaccine and 14% died before discharge. The complete recoveries were observed in 78% of cases. JE is one of the major causes of AES which is highly prevalent in this part of India. The significant findings in the present study calls for policy decision to combat JE.

Regulating Function of Exogenous Salicylic Acid on Sugar Metabolism in Tomato Seedlings

Aims: SA (Salicylic acid) is a key regulator for sugar metabolic pathway, but the regulating function on sugar metabolism in tomato seedlings is imperfect. In this study, tomato seedlings were sprayed with SA, and then soluble sugar contents, enzyme related to sugar metabolism levels, gene expressions were determined. Study Design: In order to analyze the mechanism of SA in sugar metabolic pathway, tomato seedlings were sprayed with SA, and then soluble sugar contents, enzyme related to sugar metabolism levels, gene expressions were determined. Place and Duration of Study: College of Biological Science and Technology, between December 2021 and May 2022. Methodology: The expressions of genes related to sugar metabolism in tomato were measured by qRT-PCR. The contents of soluble sugar in tomato seedings were measured with HPLC (High performance liquid chromatography). Results: The sugar metabolism in tomato seedlings was affected by SA treatment for 6 h. The contents of fructose, glucose and sucrose were improved in tomato seedlings by SA treatment. Between 6 to 24 h, enzymatic activities of SlAI, SlNI and SlSS were higher than those in controls, while there was little change in enzyme activity of SISPS. Conclusion: The contents of soluble sugar, enzyme activities related to sugar metabolism had changed considerably by SA treatment. Especially from 6 h to 24 h after SA treatment, the contents of fructose and glucose were significantly higher than those of the control group, and the changes of sugar contents were mainly related to the increases of enzyme activities of AI, NI and SS, suggesting that SA treatment mainly affected the activities of SlAI, SlNI and SlSS to promote the accumulations of soluble sugars.

A reflexividade sobre o processo de formação permanente dos gestores da atenção básica: implicações para a qualidade da assistência / Reflection on the ongoing training process of primary care managers: implications for the quality of care

A presente tese encontra-se inserida no Núcleo de Estudos Trabalho, Saúde e Educação ­ NUPETSE/EEAAC/UFF, na Linha de Pesquisa: O Cuidado em seu Contexto Sociocultural; cujo Objeto de Estudo é: O processo de formação e o desenvolvimento de competências de gestores da atenção básica de municípios do Noroeste Fluminense e suas implicações para a qualidade da assistência prestada aos usuários do Sistema Único de Saúde. Tem por Objetivos: Compreender as implicações da formação permanente de gestores da Atenção Básica do Sistema Único de Saúde em municípios do Noroeste Fluminense para a qualidade da assistência; Identificar o perfil profissional dos gestores da Atenção Básica de municípios do Noroeste Fluminense, a partir do diagnóstico situacional do cenário onde atuam; Descrever as competências necessárias para a prática profissional destes gestores; compreender de que modo a reflexividade sobre a própria prática influencia na formação profissional permanente do gestor em saúde; e, discutir sobre as implicações da formação permanente na prática profissional destes gestores e na qualidade da assistência prestada à população. Como Referencial Teórico, utilizou-se a teoria de Donald Schön, que traz a importância de uma formação baseada em um ensino reflexivo; e também Philippe Perrenoud e suas evidências de que as competências profissionais se constroem no processo de formação. Quanto à Metodologia, trata-se de uma Pesquisa descritiva, de abordagem qualitativa, do tipo Estudo de Caso. Tem como participantes gestores da Atenção Básica que atuam em quaisquer de seus programas e/ou estratégias, em municípios da Região Noroeste Fluminense. A coleta de dados se deu por meio de entrevista semiestruturada, seguindo-se da técnica de observação não participante. Foi realizado o diagnóstico situacional dos municípios estudados no intuito de contribuir para a análise reflexiva sobre a formação profissional desses gestores e suas implicações para a qualidade da assistência na Atenção Básica de Saúde. Para análise dos dados, utilizou-se a Análise de Bardin e, como recurso metodológico para dar forma ao movimento de análise, foi utilizado o Ciclo da Reflexividade, com base no movimento de ação-reflexão-ação. Dos Resultados, originaram-se três categorias: "O Conhecimento Sobre o Processo de Gestão para o Desenvolvimento de Competências"; "O papel da Instituição e do Profissional no Processo de Formação Permanente"; e "Reflexão sobre a Prática: O processo que impulsiona a qualidade da assistência". Confirma-se, portanto, a Tese que: A competência gerencial na Atenção Básica está relacionada à formação profissional permanente, baseada na reflexividade sobre a prática, com implicações positivas para a melhoria da qualidade da assistência prestada a usuários do Sistema Único de Saúde do Noroeste do Estado do Rio de Janeiro. Conclui-se que os gestores, a partir da reflexividade crítica sobre o seu processo de formação profissional, percebem a importância de torná-la permanente, como uma estratégia para o fortalecimento de habilidades e o desenvolvimento de competências gerenciais, necessárias ao trabalho na Atenção Básica.

This thesis is inserted in the Work, Health and Education Studies Center ­ NUPETSE/EEAAC/UFF, in the Research Line: Care in its Sociocultural Context; whose Object of Study is: The process of training and development of competencies for primary care managers in municipalities in the Northwest of Rio de Janeiro and its implications for the quality of care provided to users of the Unified Health System. Its Objectives: To understand the implications of the permanent training of managers of Primary Care in the Unified Health System in municipalities in the Northwest of Rio de Janeiro, for the quality of care; Identify the professional profile of Primary Care managers in municipalities in the Northwest of Rio de Janeiro, based on the situational diagnosis of the scenario in which they operate; Describe the skills needed for the professional practice of these managers; Understand how reflexivity on the practice itself influences the permanent professional training of the health manager; and, Discuss the implications of continuing education in the professional practice of these managers and in the quality of care provided to the population. As a Theoretical Framework, Donald Schön's theories were used, which brings the importance of training based on reflective teaching; and also Philippe Perrenoud, and his evidence that professional competences are built in the training process. As for the Methodology, it is a descriptive research, of the qualitative approach, case study type. Its participants are managers of Primary Care, who work in any of its programs and / or strategies; in municipalities in the Northwest Region of Rio de Janeiro. Data collection took place through semi-structured interviews, followed by the non-participant observation technique. The situational diagnosis of the municipalities studied is being carried out in order to contribute to the reflective analysis on the professional training of these managers and their implications for the quality of care in Primary Health Care. Bardin's Analysis was used for data analysis and, as a methodological resource to shape the analysis movement, the Reflexivity Cycle was used, based on the action-reflection-action movement. From the Results, three categories emerged: "Knowledge about the Management Process for the Development of Competencies"; "Whose Fault is it? The role of the Institution and the Professional in the Ongoing Training Process"; and "Reflection on Practice: The process that drives the quality of care". Therefore, the Thesis is confirmed that: Managerial competence in Primary Care is related to permanent professional training, based on reflexivity on practice, with positive implications for improving the quality of care provided to users of the Unified Health System of the Northwest of the State of Rio de Janeiro. It is concluded that managers, based on critical reflexivity on their professional training process, perceive the importance of making it permanent, as a strategy for strengthening skills and developing managerial skills, necessary for work in Primary Care.

La presente tesis se inserta en el Centro de Estudios del Trabajo, la Salud y la Educación - NUPETSE/EEAAC/UFF, en la Línea de Investigación: El cuidado en su contexto sociocultural; cuyo Objeto de Estudio es: El proceso de formación y desarrollo de competencias de los gestores de atención primaria de los municipios del noroeste fluminense y sus implicaciones para la calidad de la atención prestada a los usuarios del Sistema Único de Salud. Sus objetivos son: Comprender las implicaciones de la formación permanente de los gestores de atención primaria del Sistema Único de Salud en los municipios del noroeste fluminense, para la calidad de la atención; Identificar el perfil profesional de los gestores de atención primaria en municipios del noroeste fluminense, a partir del diagnóstico situacional del escenario donde operan; Describir las habilidades necesarias para la práctica profesional de estos gerentes; Comprender cómo la reflexividad sobre la práctica misma influye en la formación profesional permanente del gestor de la salud; y, Discutir sobre las implicaciones de la formación continua en la práctica profesional de estos gestores y sobre la calidad de la atención prestada a la población. Como Referencia Teórica se utilizó la teoría de Donald Schön, que aporta la importancia de una formación basada en una enseñanza reflexiva; y también Philippe Perrenoud, y su evidencia de que las habilidades profesionales se construyen en el proceso de capacitación. En cuanto a la Metodología, se trata de una investigación descriptiva, con un enfoque cualitativo, del tipo Estudio de Caso. Sus participantes son gerentes de atención primaria, que trabajan en cualquiera de sus programas y/o estrategias; en municipios de la Región Noroeste de Fluminense. Los datos fueron recolectados a través de entrevistas semiestructuradas, seguidas de la técnica de observación no participante. El diagnóstico situacional de los municipios estudiados se realizó con el fin de contribuir al análisis reflexivo de la formación profesional de estos gestores y sus implicaciones para la calidad asistencial en atención primaria de salud. Para el análisis de Bardin se utilizó el análisis de datos y, como recurso metodológico para dar forma al movimiento de análisis, se utilizó el Ciclo de Reflexividad, basado en el movimiento acción-reflexión-acción. A partir de los Resultados, se originaron tres categorías: "Conocimiento sobre el Proceso de Gestión para el Desarrollo de Habilidades"; "El papel de la Institución y del Profesional en el Proceso de Formación Permanente"; y "Reflexión sobre la práctica: el proceso que impulsa la calidad de la atención". Por lo tanto, se confirma la tesis de que: La competencia gerencial en Atención Primaria está relacionada con la formación profesional permanente, basada en la reflexividad sobre la práctica, con implicaciones positivas para mejorar la calidad de la atención brindada a los usuarios del Sistema Único de Salud del Noroeste del Estado de Río de Janeiro. Se concluye que los directivos, a partir de la reflexividad crítica sobre su proceso de formación profesional, perciben la importancia de hacerlo permanente, como una estrategia para el fortalecimiento de habilidades y el desarrollo de habilidades directivas, necesarias para el trabajo en Atención Primaria.

Mastering your fellowship: Part 3, 2022

The series, 'Mastering your Fellowship', provides examples of the question formats encountered in the written and clinical examinations, Part A of the Fellowship of the College of Family Physicians of South Africa (FCFP SA) examination. The series is aimed at helping family medicine registrars (and their supervisors) prepare for this examination.Keywords: family physicians; FCFP (SA) examination; family medicine registrars; postgraduate training; national exit examination; infectious diseases.

Mastering Your Fellowship: Part 4, 2022

The 'Mastering Your Fellowship' series provides examples of the question format encountered in the written and clinical examinations, Part A of the Fellowship of the College of Family Physicians of South Africa examination. The series is aimed at helping family medicine registrars prepare for this examination.

Structure-based discovery of orally efficient inhibitors via unique interactions with H-pocket of PDE8 for the treatment of vascular dementia

Our previous study demonstrated that phosphodiesterase 8 (PDE8) could work as a potential target for vascular dementia (VaD) using a chemical probe 3a. However, compound 3a is a chiral compound which was obtained by chiral resolution on HPLC, restricting its usage in clinic. Herein, a series of non-chiral 9-benzyl-2-chloro-adenine derivatives were discovered as novel PDE8 inhibitors. Lead 15 exhibited potent inhibitory activity against PDE8A (IC50 = 11 nmol/L), high selectivity over other PDEs, and remarkable drug-like properties (worthy to mention is that its bioavailability was up to 100%). Oral administration of 15 significantly improved the cAMP level of the right brain and exhibited dose-dependent effects on cognitive improvement in a VaD mouse model. Notably, the X-ray crystal structure of the PDE8A-15 complex showed that the potent affinity and high selectivity of 15 might come from the distinctive interactions with H-pocket including T-shaped π-π interactions with Phe785 as well as a unique H-bond network, which have never been observed in other PDE-inhibitor complex before, providing new strategies for the further rational design of novel selective inhibitors against PDE8.

Study on improvement of non-alcoholic steatohepatitis by regulating CDKN1A by Yiqi-Bushen-Tiaozhi formula / 中国药理学通报

Aim To screen the mechanism of Yiqi- Bushen-Tiaozhi formula ( YBTF) in treating non-alcoholic steatohepatitis ( NASH) by network pharmacology analysis and to verify it by animal experiments. Methods TCMSP database and HPLC-MS analysis were used to mine the active ingredients and targets of YBTF; GSE89632 dataset was used to screen the differential expressed genes ( DEGs) between the normal and the NASH groups; GeneCards and DisGeNET databases were used to screen NASH-related disease genes. The intersection genes of the three are the target genes of YBTF treatment of NASH. The intersection gene of the three sets of genes was the target gene of YBTF in treating NASH. GO, KEGG, DO enrichment analysis, protein-protein interaction network, and network topology analysis were used to identify the hub genes of YBTF in the treatment of NASH. Molecular docking was used to judge whether cmcial target genes, active ingredients could be combined and exer ted a curative effect; Oil red 0 and HE staining were used to determine whether YBTF could treat NASH mice; (3-galactosidase ( SA- (3-Gal) test was used to determine whether NASH mice had hepatocyte senescence and whether YBTF improved senescence; West-ern blot. Quantitative Real-time PGR ( qRT-PCR) combined with sequencing results were used to verify whether YBTF could regulate the expression of the essential target genes screened from the protein and RNA levels. Results YBTF could improve cellular aging and treat NASH through CDKN1A. Conclusion The rational application of Traditional Chinese medicine (TCM) network pharmacology and experiments can provide new ideas and directions for studying the mechanism of YBTF.

Covid-19 and mental health: A study of social representations with university students / Covid-19 y salud mental: un estudio de representaciones sociales con estudiantes universitários / Covid-19 e saúde mental: um estudo de representações sociais com universitários

The objective was to apprehend the Social Representations (SR) of university students from a private Institution about Covid-19 and mental health. There were 294 participants, between 18 to 59 years old (M = 27.43; SD = 6.05). These participants submitted to a sociodemographic questionnaire and the Free Word Association Technique (stimuli: coronavirus and health mental), applied online. Process the data using the TRIDEUX-MOTS software through the Factor Analysis of Correspondence. The results pointed to consensualities between the "coronavirus" and "mental health" stimuli anchored in the psycho-emotional sphere, aimed at anxiety, fear, anguish, and sadness. These stimuli also share other nuclei of a meaning of SR, anchored in protective elements of mental health, faith, health, care, and family. Thus, understanding how SR from different groups in the face of this new phenomenon can help preventive actions to face the disease and preserve mental health.

Objetivou-se apreender as Representaš§es Sociais (RS) de estudantes universitßrios de uma instituišNo privada sobre Covid-19 e sa?de mental. Contou-se com a participašNo de 294 participantes, entre 18 a 59 anos (M= 27,43; DP= 6,05), os quais foram submetidos a um questionßrio sociodemogrßfico e Ó TÚcnica de AssociašNo Livre de Palavras (estÝmulos: coronavÝrus e sa?de mental), aplicados de forma online. Processou-se os dados pelo software TRIDEUX-MOTS, por meio da Anßlise Fatorial de Correspondrncia. Os resultados apontaram para consensualidades entre os estÝmulos "coronavÝrus" e "sa?de mental" ancorados na esfera psicoemocional, objetivadas por: ansiedade, medo, ang?stia e tristeza. Esses estÝmulos tambÚm compartilharam outros n?cleos de sentido das RS, ancorados em elementos protetivos da sa?de mental, sendo eles: fÚ, sa?de, cuidado e famÝlia. Assim, compreender as RS de diferentes grupos diante deste novo fen?meno pode auxiliar aš§es preventivas de enfrentamento da doenša e preservašNo da sa?de mental.

El objetivo era aprehender a las Representaciones Sociales (RS) de estudiantes universitarios de una instituci?n privada sobre Covid-19 y salud mental. P?ngase en contacto con la participaci?n de 294 participantes, entre 18 y 59 a?os (M = 27.43; SD = 6.05), cußles son los requisitos para el cuestionario sociodemogrßfico y la tÚcnica de asociaci?n de palabras libres (estÝmulos: coronavirus y salud mental), aplicaciones en linea. Procese los datos usando el software TRIDEUX-MOTS, a travÚs del Anßlisis de Correspondencia de Factor. Los resultados mostraron consensualidades entre el "coronavirus" y los estÝmulos de "salud mental" anclados en la esfera psicoemocional, con el objetivo de: ansiedad, miedo, angustia y tristeza. Estos estÝmulos tambiÚn comparten otros n?cleos de significado SR, anclados en elementos protectores de la salud mental, son: fe, salud, cuidado y familia. Por lo tanto, comprender c?mo la RS de diferentes grupos frente a este nuevo fen?meno puede ayudar a las acciones preventivas para enfrentar la enfermedad y preservar la salud mental.

Investigando a Experirncia de usußrios em um Centro de Convivrncia sob a perspectiva da psicologia humanista e da fenomenologia / Investigating the Experience of users in a Coexistence Community Center from the perspective of humanistic psychology and phenomenology / Investigando la Experiencia de usuarios en un Centro de Convivencia desde la perspectiva de la psicologÝa humanista y la fenomenologÝa

Este artigo apresenta os resultados de uma pesquisa qualitativa de natureza fenomenol?gica que objetivou compreender a experirncia de pessoas que frequentam um Centro de Convivrncia (CECO). Foram realizados encontros dial?gicos individuais com sete usußrios adultos, de ambos os sexos. Ap?s cada encontro, o pesquisador redigiu uma Narrativa Compreensiva, a partir de suas pr?prias impress§es, sobre a experirncia do (a) participante. Uma Narrativa SÝntese foi construÝda, em seguida, contendo os elementos significativos da experirncia de todos os participantes em relašNo Ós vivrncias no CECO. Os elementos fenomenologicamente desvelados, que constituÝram os resultados da pesquisa, podem ser assim expressos: (1) as relaš§es interpessoais, mediadas pelos profissionais no contexto do CECO, sNo orientadas por respeito, compreensNo e interesse m?tuo; (2) a partir da convivrncia cotidiana com outras pessoas e da participašNo em atividades coletivas, os usußrios podem desenvolver criativamente suas habilidades e interesses; e (3) ao se sentirem acolhidos e respeitados como pessoas, os usußrios tambÚm desenvolvem uma relašNo afetiva positiva com o servišo. Concluindo, o CECO Ú um espašo propÝcio ao desenvolvimento de potencialidades individuais e coletivas e Ó valorizašNo de relaš§es sociais construtivas que facilitam e preservam a tendrncia inerente das pessoas para o crescimento, a autonomia e o amadurecimento psicol?gico.

This paper presents the results of a qualitative research of phenomenological nature that aimed to understand the experience of people who attend a Coexistence Community Center (CECO). Individual dialogical encounters were held with seven adult users of both sexes. After each meeting, the researcher wrote a Comprehensive Narrative, based on his own impressions, about the participant's experience. A Narrative Synthesis was then constructed containing the significant elements of the experience of all participants in relation to the experiences in the CECO. The phenomenologically revealed elements, that constituted the results of the research, can be expressed as follows: (1) interpersonal relations, mediated by professionals in the context of the CECO, are guided by respect, understanding and mutual interest; (2) from the daily coexistence with other people and from participation in collective activities, users can creatively develop their skills and interests; and (3) when they feel welcomed and respected as people, users also develop a positive affective relationship with the service. In conclusion, CECO is a space conducive to the development of individual and collective potentialities and to the valorization of constructive social relations that facilitate and preserve the inherent tendency of people for growth, autonomy and psychological maturation.

Este artÝculo presenta los resultados de una investigaci?n cualitativa de naturaleza fenomenol?gica que objetiv? comprender la experiencia de personas que frecuentan un Centro de Convivrncia (CECO). Se realizaron encuentros dial?gicos individuales com siete usußrios adultos de ambos sexos. DespuÚs de cada encuentro, el investigador redact? una Narrativa Comprensiva, a partir de sus propias impresiones, sobre la experiencia del participante. A continuaci?n, una Narrativa SÝntesis fue construÝda conteniendo los elementos significativos de la experiencia de todos los participantes en relaci?n a las vivencias en el CECO. Los elementos fenomenol?gicamente desvelados, que constituyeron los resultados de la investigaci?n, pueden ser asÝ expresados: (1) las relaciones interpersonales mediadas por los profesionales en el contexto del CECO se orientan por respeto, comprensi?n y interÚs mutuo; (2) a partir de la convivencia cotidiana con otras personas y de la participaci?n en actividades colectivas, los usuarios pueden desarrollar creativamente sus habilidades e intereses; y (3) al sentirse acogidos y respetados como personas, los usuarios tambiÚn desarrollan una relaci?n afectiva positiva con el servicio. Concluyendo, el CECO es un espacio propicio para el desarrollo de potencialidades individuales y colectivas y la valorizaci?n de relaciones sociales constructivas que facilitan y preservan la tendencia inherente de las personas hacia el crecimiento, la autonomÝa y la maduraci?n psicol?gica.

Facile synthesis of insulin fusion derivatives through sortase A ligation

Insulin derivatives such as insulin detemir and insulin degludec are U.S. Food and Drug Administration (FDA)-approved long-acting insulin currently used by millions of people with diabetes. These derivatives are modified in C-terminal B29 lysine to retain insulin bioactivity. New and efficient methods for facile synthesis of insulin derivatives may lead to new discovery of therapeutic insulin. Herein, we report a new method using sortase A (SrtA)-mediated ligation for the synthesis of insulin derivatives with high efficiency and functional group tolerance in the C-terminal B chain. This new insulin molecule (Ins-SA) with an SrtA-recognizing motif can be conjugated to diverse groups with N-terminal oligoglycines to generate new insulin derivatives. We further demonstrated that a new insulin derivative synthesized by this SrtA-mediated ligation shows strong cellular and

Sodium Arsenite Inhibits Proliferation and Induces Apoptosis of Human Hepatic Cells / 中国生物化学与分子生物学报

Arsenic is a potent toxic heavy metal found in the environment that can causes health problems, including liver disease in humans and animals. Chronic exposure to arsenic remains an environmental health problem worldwide, affecting hundreds of millions of people. Although the oxidative stress and apoptosis induced by arsenic have been confirmed, the underlying mechanism of apoptosis has not been fully elucidated. The purpose of this study is to investigate whether sodium arsenite (SA)induced liver toxicity is related to the regulation of DNA replication and repair pathways. The results of MTT and microscopy showed that SA has an inhibitory effect on the proliferation of human hepatocytes (L02), and this effect is time and concentration dependent. Flow cytometry detected the effects of different concentrations of SA on L02 cells. Compared with the control group, high concentrations of SA significantly affected the L02 cell cycle. In addition, RNA sequencing results showed that the differentially expressed genes in cells after SA treatment were concentrated in the DNA replication process and repair pathways. The effect of SA treatment on the expression of human RECQ DNA helicase and repair genes was further confirmed by Western blot and real-time quantitative PCR. In vitro study showed that SA treatment inhibited cell proliferation, and induced apoptosis as well as DNA damage and cell cycle arrest of human liver cell L02. Collectively, these results indicate that arsenic poisoning is related to the regulation of DNA replication and repair pathways, which provides insight for understanding the molecular mechanism of arsenic poisoning.

O acesso à informação nos arquivos de Angola: fundo da antiga escola industrial e comercial Artur de Paiva (1939-1975) / Access to information in Angola's archives: fund of the old industrial and commercial school Artur de Paiva (1939-1975)

RESUMO O ensaio cuja abordagem centra-se no "acesso à informação nos arquivos de Angola: fundo da antiga Escola Industrial e Comercial Artur de Paiva", limitado em termos cronológicos nos períodos entre 1939 a 1975. É interessante estudar o acesso à informação deste fundo pelas seguintes razões: Por um lado, a escola sendo uma das mais antigas da Região Sul de Angola, e por outro lado, devido ao elevado número de documentos de valor histórico e investigativo acumulado ao longo destes anos pela instituição. Daí que, a necessidade de analisar o estado atual do acesso à informação nos arquivos de Angola e da legislação existente sobre ela, virada para o sector de ensino, tornou-se o principal objetivo do ensaio. Far-se-á através de um estudo de caso, documental e bibliográfica, analisando o fenómeno isoladamente para percebermos melhor a situação real do acesso à informação na instituição. A combinação de vários elementos metodológicos, permitirá perceber o mesmo objeto sobre perspetivas diferentes. Efetivamente, também serão apresentadas legislações nacionais e internacionais que tratam sobre o acesso à informação, proteção dos dados pessoais, entre outros. Finalmente, será possível propor um modelo para o acesso à informação do fundo histórico da escola.

ABSTRACT The tes whose approach focuses on "Access to information in Angola's archives: fund of the old Industrial and Commercial School Artur de Paiva", limited in chronological terms between 1939 and 1975. It is interesting to study the access to information from this fund by following reasons: On the one hand, the school being one of the oldest in the Southern Region of Angola, and on the other hand, due to the high number of documents of historical and investigative value accumulated over these years by the institution. Hence, the need to analyze the current state of access to information in the archives of Angola and the existing legislation on it, facing the education sector, became the main objective of the essay. It will be done through a case study, documentary and bibliographic, analyzing the phenomenon in isolation to better understand the real situation of access to information in the institution. The combination of several methodological elements, will allow to perceive the same object from different perspectives. Effectively, national and international laws that deal with access to information, protection of personal data, among others, will also be presented. Finally, it will be possible to propose a model for accessing information from the school's historical fund.

RESUMEN El ensayo cuyo enfoque se centra en "El acceso a la información en los archivos de Angola: Fondo de la antigua Escuela Industrial y Comercial Artur de Paiva", limitada en términos cronológicos entre 1939 y 1975..Es interesante estudiar el acceso a la información de este fondo por las siguientes razones: por un lado, la escuela es una de las más antiguas de la Región Sur de Angola, y por otro lado, debido a la gran cantidad de documentos de valor histórico e investigativo acumulados durante estos años. años por la institución. Por lo tanto, la necesidad de analizar el estado actual del acceso a la información en los archivos de Angola y la legislación existente sobre el mismo, frente al sector educativo, se convirtió en el objetivo principal del ensayo. Se realizará a través de un estudio de caso, documental y bibliográfico, analizando el fenómeno de forma aislada para comprender mejor la situación real de acceso a la información en la institución. La combinación de varios elementos metodológicos, permitirá percibir el mismo objeto desde diferentes perspectivas. Efectivamente, también se presentarán leyes nacionales e internacionales que se ocupan del acceso a la información, la protección de datos personales, entre otros. Finalmente, será posible proponer un modelo para acceder a la información de los antecedentes históricos de la escuela.

O enfrentamento da violência contra as mulheres no âmbito da estratégia de saúde da família / Facing violence against women in the framework of the family health strategy / El enfrentamiento de la violencia contra las mujeres en el ámbito de la estrategia de salud de la familia

Este artigo teve como objetivo identificar aš§es de enfrentamento da violrncia contra as mulheres pela EstratÚgia de Sa?de da FamÝlia (ESF). Foi realizada uma revisNo integrativa dos artigos publicados entre 2011 e 2016, encontrados nas seguintes bases: Literatura Latino-Americana e do Caribe em Cirncias da Sa?de (Lilacs), Base de Dados em Enfermagem (Bdenf), Index Psicologia - Peri?dicos tÚcnico-cientÝficos e Rede Pan-Americana de InformašNo e DocumentašNo em Engenharia Sanitßria e Cirncias do Ambiente (Repidisca). Foram encontrados 29 artigos, dos quais 13 foram selecionados para anßlise segundo as categorias: "o trabalho em rede com mulheres em situašNo de violrncia" e "limites e necessidades profissionais para o enfrentamento da violrncia de grnero". A revisNo mostra lacunas na formašNo dos profissionais de sa?de para o diagn?stico e intervenšNo nos casos de violrncia de grnero, e desarticulašNo entre a rede de protešNo social e das equipes de sa?de da famÝlia no atendimento das mulheres vÝtimas de violrncia.

This article aimed to identify actions in the Family Health Strategies (FHS) to face violence against women. An integrative review of articles published between 2011 and 2016 was carried out in the following databases: Latin American and Caribbean Literature in Health Sciences (Lilacs), Nursing Database (Bdenf), Index Psychology - Technical-Scientific Periodicals and Network Pan American Institute of Information and Documentation in Sanitary Engineering and Environmental Sciences (Repidisca). Thirteen (13) out of the twenty-nine (29) articles we studied were selected for analysis according to the categories: "network with women in situation of violence" and "limits and professional needs to face gender violence". The review shows failures in the training of health professionals for the diagnosis and intervention in cases of gender violence, and lack of connection between the social protection network and the family health teams in the care of women victims of violence.

Este artÝculo objetiv? identificar acciones de enfrentamiento de la violencia contra las mujeres por las Estrategias de Salud de la Familia (ESF). Se realiz? una revisi?n integrativa de los artÝculos publicados entre 2011 y 2016, encontrados en las siguientes bases: Literatura Latinoamericana y del Caribe en Ciencias de la Salud (Lilacs), Base de datos en EnfermerÝa (Bdenf), Index PsicologÝa - Peri?dicos tÚcnico-cientÝficos y Red Panamericana de Informaci?n y Documentaci?n en IngenierÝa Sanitaria y Ciencias del Ambiente (Repidisca). Se encontraron 29 artÝculos, de los cuales 13 fueron seleccionados para anßlisis seg?n las categorÝas: "el trabajo en red con mujeres en situaci?n de violencia" y "lÝmites y necesidades profesionales para el enfrentamiento de la violencia de gÚnero". La revisi?n muestra lagunas en la formaci?n de los profesionales de la salud para el diagn?stico e intervenci?n en los casos de violencia de gÚnero, y desarticulaci?n entre la red de protecci?n social y de los equipos de salud de la familia en la atenci?n a las mujeres vÝctimas de violencia.

Características clínicas y OCT-SA de pacientes con síndrome de tracción iridiana asociado a desprendimiento de retina regmatógeno. Reporte de tres casos / Clinical features and AS-OCT of patients with iris retraction syndrome following rhegmatogenous retinal detachment. Report of three cases

Introducción: se presenta una serie de 3 casos de síndrome de retracción iridiana (SRI) asociados a desprendimiento de retina regmatógeno (DRR) y desprendimiento coroideo. Objetivo: dar a conocer las características clínicas y tomográficas de tres pacientes con SRI asociado a desprendimiento de retina regmatógeno. Diseño del estudio: serie de casos. Resumen del caso: tres pacientes que presentaron DRR asociado a SRI. El tiempo de evolución promedio fue de 21 días (15-30 días), 2 de los 3 pacientes tuvieron resolución del SRI posterior al inicio de midriáticos y antes de ser intervenidos por el desprendimiento de retina, y 2 tuvieron presencia de proliferación vitreoretiniana (PVR) a pesar del corto tiempo de evolución. Adicionalmente estos 2 pacientes cursaron con desprendimientos coroideos. Todos los pacientes tuvieron un buen resultado estructural, AV menor o igual a 20/400, no redesprendimientos ni recurrencias de SRI durante el seguimiento. Conclusión: ante un paciente con SRI en el cual no sea posible valorar polo posterior, se debe tener en cuenta el diagnóstico de desprendimiento de retina regmatógeno. El SRI puede considerarse como un factor de mal pronóstico en pacientes con desprendimiento de retina regmatógeno.

Background: to report a 3 cases series of patients with iris retraction syndrome (IRS). Objective: introduce the clinical and tomographic features of tree patients with IRS following RRD. Study design: case series. Case summary: three patients presented IRS following RRD. They presented with an average evolution of 21 days (15 to 30 days), 2 patients had resolution of the IRS after mydriatics and before receiving surgical treatment for retinal detacthment, and 2 had vitreoretinal proliferation despite the short time. Besides, these 2 patients had choroidal detachments discovered in the operating room. All patients had good structural outcomes, visual acuity less or equal than 20/400, no re-detachment of the retina and no recurrence of IRS during follow up. Conclusion: the presence of a retinal detachment should be suspected in a patient with IRS in which it´s not possible to assess the posterior pole, and it should be considered a bad prognostic factor if present.

Analysis of WCID-cIEF Spectrogram of Recombinant Interleukin-15 Fusion Protein / 中国药学杂志

OBJECTIVE: To analyze the glycosylated chains of recombinant interleukin-15 fusion protein using capillary isoelectric focusing-whole column imaging detection (WCID-cIEF) spectrograms. METHODS: Using established corresponding mathematical models and the least square method, the WCID-cIEF spectrograms of whole protein, de-salicylic-acid protein and de-N-glycosylation-chain protein were analyzed. Among the mathematical models, the interval-1-peak model was selected. And according to the model, the relationship between isoform peak-areas and isoelectric points was listed. RESULTS: The rationality of the interval-1-peak model was confirmed and a series of basic data was obtained according to the model as follows:the apparent m value of the protein was 25.53 reference(R), the apparent n value of the protein was 28.83R, the apparent m value of sialic acid was 0.86 (0.855) R, the apparent n value was 0.12 (0.119) R, the apparent n value of N-acetylglucosamine (undifferentiated from N-acetylgalactosamine) was 0.06(0.061) R, and the apparent m value of formed carboxyl after N-chain removal was 0.19 (0.186) R. Some information of protein sugar composition was also obtained: the sialylation degree was about 1.83 mol•mol-1, the percentage of prototype protein was about 8.3%, the percentage of single N-glycosated modification protein was about 19.8%, the percentage of double N-glycosated modification protein was about 28.4%, the percentage of triple N-glycosated modification protein was about 23.7%, and the percentage of O-glycosated modification (with sialic acid) protein was about 19.8%. The main sugar types should be G0 (F), G1 (F), G2 (F), G1A1 (F), and G2A1 (F). CONCLUSION: The structure of sugar chain is complex, but it also has some repeatability and regularity. We hope that through this study, the glycoprotein sugar chain can be quickly outlined, the understanding of glycoprotein and the study of protein interaction can be improved.

Frecuencia de factores de riesgo cardiovascular en pacientes con síndrome isquémico coronario agudo, Chiclayo / Frequency of risk factors for cardiovascular disease among patients with acute coronary syndrome, Chiclayo

Objetivo: Determinar la frecuencia de factores de riesgo cardiovasculares en pacientes con Síndrome coronario agudo (SCA) de dos hospitales de Chiclayo. Material y métodos: Se realizó un estudio descriptivo transversal. Participaron 100 pacientes hospitalizados con SCA, se les aplicó una ficha de recolección de información que permitió registrar antecedentes de factores de riesgo cardiovascular. También se utilizó la escala ronquido somnolencia y escala de somnolencia de Epworth (versión peruana) para evaluar síntomas relacionados a apnea de sueño. Resultados: Los factores de riesgo cardiovascular más frecuentes fueron: Obesidad según índice cintura cadera: 87/88 (98,86%), edad mayor de 55 años en varones y de 65 años en mujeres: 78/100 (78%), hipertensión arterial: 71/100 (71%), dislipidemia: 54/97 (55,67%) y sedentarismo: 49/97 (50,51%). Se encontró mayor frecuencia de edad avanzada, hipertensión arterial, sedentarismo, obesidad abdominal en mujeres y de tabaquismo en hombres. Conclusiones: El factor de riesgo cardiovascular más frecuente fue la obesidad según índice cintura-cadera y el menos frecuente fue el tener antecedente familiar de SICA.

Objective: To determine the frequency of risk factors for cardiovascular disease among patients with acute coronary syndrome (ACS) in two hospitals in Chiclayo. Methods: A cross-sectional study was carried-out among 100 admitted patients with ACS, a survey was applied to gather information on risk factors for cardiovascular disease. To evaluate symptoms associated with sleep-apnea we applied two scales, the Peruvian version of the Epworth somnolence scale and the snoring-somnolence scale. Results: the most frequent risk factors were abdominal obesity 87/88 (98.86%), age above 55 years in males and 65 years in females: 78/100 (78%), blood hypertension: 71/100 (71%), dyslipidemia: 54/97 (55.67%) and sedentarism: 49/97 (50.51%). Advanced age, blood hypertension, sedentarism and abdominal obesity were more common in females, while smoking was more common in males. Conclusions: The most frequent risk factor was abdominal obesity and the less frequent was family history of ACS.

Utilidad del índice triglicéridos/HDL-C desde los primeros años de vida en el diagnóstico de síndrome metabólico en niños obesos / Utility of the triglycerides/HDL-C index from the first years of age in the diagnosis of metabolic syndrome in obese children

Objetivo: Determinar la utilidad del índice triglicéridos/HDL-C (TG/HDL-C) para el diagnóstico de síndrome metabólico (SM) en niños obesos de 2 a 14 años. Material y métodos: Estudio transversal tipo prueba de diagnóstico. Fueron incluidos 360 niños obesos exógenos (199M/161F), divididos en tres grupos etarios: 2 a <6 años, 6 a <10 años y 10 a 14 años. Se definió SM según los criterios de la International Diabetes Federation y se evaluó al índice TG/HDL-C como diagnóstico en dos puntos de corte: ≥2,32 y ≥3,5, en cada grupo etario. Se aplicó Chi cuadrado, considerándose significativo p<0,05. Se determinó la sensibilidad, especificidad y valores predictivos positivo y negativo, para cada punto de corte. Resultados: La frecuencia de SM fue 15,79% de 2 a <6 años, 20,25% de 6 a <10 años, 19,63% de 10 a 14 años. En los niños con SM el 97,1% presentó HDL bajo, 83,8% triglicéridos elevados. Se encontró diferencia significativa en la frecuencia del índice TG/HDL-C en ambos puntos de corte, entre los niños con y sin SM en todos los grupos etarios. La sensibilidad para ambos puntos de corte fue alta (86-100%) y la especificidad fue mejor para el punto de corte ≥3,5 (72-80%). Conclusiones: El índice TG/HDL-C ≥3,5 representaría un marcador sensible y específico para el diagnóstico de síndrome metabólico desde los primeros años de vida.

Objective: To determine the utility of the triglycerides/HDL-C index for the diagnosis of metabolic syndrome (MS) in obese 2-14 years of age children. Methods: Cross-sectional diagnostic study. We included 360 exogenous obese children (199M/161F) divided in three age groups: 2 to <6; 6 to <10 and 10-14 years. MS was defined according to the International Diabetes Federation and the TG/HDL-C index was evaluated using two cutoffs by age group, ≥2.32 and ≥3.5. Chi squared was used accepting a p value <0.05 as statistically significant. Fir each cut-off we determined sensitivity, specificity and predictive values. Results: Frequency of MS was 15.79% in the 2 to <6 years age group; 20.25% in the 6 to<10 years age group and 19.63% in the 10-14 years age group. Among children with SM, 97.1% had low HDL and 83.8% had elevated triglycerides. A statistically significant difference was found in the frequency of the TG/HDL-C index in children with or without MS in all age groups. Sensitivity for all cut-offs was high (86-100%) and specificity was best for the cut-off of ≥3.5 (72-80%). Conclusions: The TG/HDL-C index is a sensitive and specific marker of MS from the first years of age.