Áreas geográficas con mayor concentración de accidentes de tránsito en San Salvador, El Salvador: un análisis espacial del periodo 2014-2018 / Geographic areas with the highest concentration of traffic accidents in San Salvador, El Salvador: a spatial analysis of the 2014-2018 period

RESUMEN Objetivo. Identificar las áreas de mayor concentración de accidentes de tránsito y lesionados en el Área Metropolitana de San Salvador (AMSS). Materiales y métodos. Los accidentes de tránsito se analizaron espacialmente mediante la ubicación puntual y por la sumatoria de eventos en áreas de 200 m2. La ubicación puntual se analizó mediante «análisis de vecinos más cercanos¼, mientras que las áreas con la sumatoria de accidentes de tránsito se analizaron mediante Gi* de Getis-Ord para obtener los puntos calientes. Los puntos calientes resultantes con mayor concentración de accidentes de tránsito en el AMSS se evaluaron en campo mediante un formulario de observación de las características de infraestructura y seguridad vial. Resultados. Al analizar 8191 accidentes de tránsito reportados entre 2014‒2018, se identificaron cinco áreas con mayor cantidad de accidentes de tránsito y lesionados, principalmente sobre vías primarias. Conclusión. Los sitios de mayor concentración de accidentes de tránsito y lesionados se caracterizan por una infraestructura vial con daños considerables y falta de sistemas de seguridad para conductores y peatones. El análisis espacial de los accidentes de tránsito y lesionados puede contribuir a mejorar la vigilancia y seguridad vial en el AMSS.

ABSTRACT Objective. This study aimed to identify the areas with the highest concentration of traffic accidents and injuries in the San Salvador Metropolitan Area (SSMA). Materials and methods. Traffic accidents were analyzed spatially by point location and by the sum of events in areas of 200 m2. The point location was analyzed by "nearest neighbor analysis", while the areas with the sum of traffic accidents were analyzed by Getis-Ord Gi* to obtain the hot spots. The resulting hot spots with the highest concentration of traffic accidents in the SSMA were evaluated in the field using an observation form to collect data on infrastructure and road safety characteristics. Results. Five areas with the highest number of traffic accidents and injuries, mainly containing primary roads, were identified by analyzing 8191 traffic accidents reported between 2014-2018. Conclusion. The sites with the highest concentration of traffic accidents and injuries were characterized by considerably damaged road infrastructure and the lack of safety systems for drivers and pedestrians. The spatial analysis of traffic accidents and injuries can contribute to improve surveillance and road safety in the SSMA.

La inteligencia artificial y la video-vigilancia en la predicción y detección de delitos en espacio-tiempo: una revisión sistemática / Artificial intelligence and video surveillance in space-time crime prediction and detection: a systematic review / Inteligência artificial e vídeo-vigilância na previsão e detecção de crimes no espaço-tempo: uma revisão sistemática

En la sociedad de hoy los delitos vienen incrementándose y particularmente en la ciudad de Bogotá, lo que ha causado muchos inconvenientes a la Policía Nacional de Colombia, así como también a los centros de seguridad ciudadana. Ante esta situación, se ha propuesto una predicción de tiempo-espacio en los puntos críticos de crímenes y delitos, con la ayuda de inteligencia artificial. Por consiguiente, este trabajo tiene como objetivo analizar, resumir, interpretar y evaluar las distintas técnicas de predicción espacio-temporal de la delincuencia con un panorama inteligente. Por la propia naturaleza de la investigación, se utilizó una metodología de enfoque descriptivo-cualitativo, con la cual se diseñaron fichas de observación estructurada para sistematizar información de cinco bases de datos: Scopus, Web of Science, IEEE, ACM, Springer; dichas publicaciones comprenden desde 2019 hasta junio de 2021. En consecuencia, se encontraron en total 3015 estudios, después del proceso de cribado y verificación de los criterios de exclusión e inclusión, se seleccionaron 132 artículos, luego se aplicaron preguntas Psicólogo Interno Residente (PIR), quedando así 18 artículos. Los principales hallazgos encontrados indican que los algoritmos de redes neuronales resultaron ser uno de los métodos más eficaces para la detección de puntos críticos de delincuencia, dado que los grandes avances de la tecnología coadyuvarían en los próximos años a predecir de forma rápida y eficaz los actos delictivos y los crímenes ubicados en cualquier región del continente latinoamericano.

In today's society, crimes are increasing, particularly in the city of Bogota, which has caused many inconveniences to the National Police of Colombia, as well as to the citizen security centers. Given this situation, a time-space prediction of crime and crime hotspots has been proposed with the help of artificial intelligence. Therefore, this paper aims to analyze, summarize, interpret and evaluate the various techniques of space-time prediction of crime with an intelligent view. Due to the very nature of the research, a descriptive-qualitative approach methodology was used, with which structured observation sheets were designed to systematize information from five da-tabases: Scopus, Web of Science, IEEE, ACM, Springer; these publications span from 2019 to June 2021. Consequently, a total of 3015 studies were found, after the screening process and verification of exclusion and inclusion criteria, 132 articles were selected, then questions were applied Psychologist Internal Resident (PIR), thus leaving 18 articles. The main findings indicate that neural network algorithms proved to be one of the most effective methods for the detection of crime hotspots, given that the great advances in technology would help in the coming years to quickly and effectively predict criminal acts and crimes located in any region of the Latin American continent.

Na sociedade de hoje, a criminalidade está aumentando, particularmente na cidade de Bogotá, o que tem causado muitos inconvenientes para a Polícia Nacional Colombiana, bem como para os centros de segurança do cidadão. Diante desta situação, foi proposta uma previsão tempo-espacial de hotspots de crime com a ajuda da inteligência artificial. Portanto, este documento visa analisar, resumir, interpretar e avaliar as diversas técnicas de previsão espaço-temporal do crime com uma visão inteligente. Devido à própria natureza da pesquisa, foi utilizada uma metodologia de abordagem descritiva-qualitativa, com a qual foram elaboradas fichas de observação estrutura-das para sistematizar informações de cinco bancos de dados: Scopus, Web of Science, IEEE, ACM, Springer; estas publicações abrangem o período de 2019 a junho de 2021. Consequentemente, foi encontrado um total de 3015 estudos, após o processo de triagem e verificação dos critérios de exclusão e inclusão, 132 artigos foram selecionados, depois foram aplicadas perguntas ao Psicólogo em Residência (PIR), deixando 18 artigos. As principais descobertas indicam que os algoritmos de redes neurais provaram ser um dos métodos mais eficazes para a detecção de hotspots de crime, dado que os grandes avanços na tecnologia ajudarão nos próximos anos a prever rápida e efetivamente atos criminosos e crimes localizados em qualquer região do continente latino-americano.

Geographic Information Systems based approach for assessing the locational feasibility for biomethane production from landfill gas and injection in pipelines in Brazil / Abordagem baseada em sistemas de informação geográfica para avaliação da viabilidade locacional da produção de biometano a partir de gás de aterro sanitário e injeção em dutos no Brasil

ABSTRACT Biomethane can readily replace fossil fuels including natural gas, which has similar physical and chemical properties. In Brazil, municipal solid waste is predominantly disposed of in landfills. Landfill gas is mostly employed for electricity generation, but still at low levels when compared to the existing potential. Production of biomethane from landfill gas may be an alternative to exploit the existing potential, but Brazil's pipeline network is rather limited and concentrated along the country's coast. In this context, the research sought to identify the locational viability of using landfill gas to produce biomethane and injecting it into pipelines, considering the available potential and its proximity to Brazil's existing pipeline network. The QGis software was used to integrate the information. Territorial arrangements with a biomethane production capacity of more than 15,000 Nm3 day−1 and located up to 50 km from the pipeline network were considered feasible. The research estimated a potential production equivalent to 3,407,027 Nm3 day−1 of biomethane from landfills in Brazil. This potential corresponds to 6% of country's natural gas consumption in 2019 and is almost 32 times greater than current production of biomethane from all substrates used with this purpose in that year. The results indicate the suitability of using geographic information systems to identify regions that can benefit from the production of biomethane from landfill gas using the existing natural gas pipelines as an alternative to the electricity generation and provides relevant subsidies to the formulation of more efficient public policies in both the sanitation and energy sectors.

RESUMO O biometano pode substituir facilmente os combustíveis fósseis, incluindo o gás natural, que possui propriedades físicas e químicas similares. No Brasil, os resíduos sólidos urbanos são descartados predominantemente em aterros sanitários. O gás dos aterros sanitários é empregado principalmente na geração de eletricidade, mas ainda em níveis baixos quando comparado ao potencial existente. A produção de biometano a partir do gás de aterro pode ser uma alternativa para explorar o potencial existente, mas a rede de gasodutos do Brasil é bastante limitada e concentrada ao longo da costa do país. Nesse contexto, esta pesquisa buscou identificar a viabilidade locacional do uso de gás de aterro sanitário para produzir biometano e injetá-lo em dutos, considerando o potencial disponível e sua proximidade com a rede de dutos existente no Brasil. O software QGis foi utilizado para integrar as informações. Foram considerados viáveis arranjos territoriais com uma capacidade de produção de biometano maior que 15.000 Nm3 dia−1 e localizados a até 50 km da rede de gasodutos. A pesquisa estimou uma produção potencial equivalente a 3.407.027 Nm3 dia−1 de biometano a partir de aterros sanitários no Brasil. Esse potencial corresponde a 6% do consumo de gás natural do país em 2019 e é quase 32 vezes maior que a produção de biometano de todos os substratos utilizados com essa finalidade naquele ano. Os resultados indicam a adequação do uso de sistemas de informação geográfica para identificar regiões que podem se beneficiar da produção de biometano a partir de gás de aterro sanitário, utilizando os gasodutos de gás natural existentes como alternativa à geração de eletricidade e fornece subsídios relevantes para a formulação de políticas públicas mais eficientes, tanto no setor de saneamento quanto no de energia.

6-desulfated heparin inhibits heparan sulfate shedding and epithelial cell damage during bleomycin induced pulmonary injury / 中国药理学通报

Aim To study the effect of different hepa- ry.Methods First, heparin derivatives with different rin sulfation patterns on bleomycin induced lung inju- sulfation patterns,6-desulfated heparin (6-DeH) and N-acetvlated heparin ( N-AcH ) , were synthesized.Secondly, the effect of these compounds on BLM-in¬duced bronchial epithelial cell ( BEARS-2B) injury was evaluated via lactate dehydrogenase activity, MTT experiment, Annexin V/ PI staining and Hoechst 33258 staining.Then , immunofluorescence staining and West¬ern blotting were used to investigate the shedding of Svndecan-1 and the activation of c-Met by 6-DeH/Akt j j signaling pathway.Finally, a BLM-induced lung injury mouse model was used to further verify the protective effect of 6-DeH by HE staining, Svndecan-1 immunos- taining,bodv weight change,and survival rate.Results In the BLM-induced BEARS-2B injury model, 6- DeH was selected as the best candidate, which exerted their effect by competitively binding to BLM, thereby reducing the damage of heparan sulfate barrier (Svnde- can-1 ) on cell surface, and improving cell survival by activating the downstream c-Met/Akt pathway.In the BLM-induced lung injury mouse model, it was further confirmed that 6-DeH reduced the shedding of Svnde- can-1 in the early stage, and delayed the lung injury and fibrosis process.Conclusions 6-DeH protects the bronchial epithelial cells against BLM-induced lung in¬jur)' through inhibiting the shedding of Svndecan-1 and activating the c-Met/Akt signaling pathway.

Regulation of the Sigma-1 Receptor on Chronic Pain and Its Neuromechanism / 中国生物化学与分子生物学报

Sigma-1 receptor (Sig-1R) is a ligand-regulated chaperone protein widely expressed in multiple regions of the nervous system. It mediates intracellular effects of several types of ion channels and G protein coupled receptors (GPCRs) by binding them, and regulates intracellular Ca

Staphylococcal cassette chromosome mec elements from Staphylococcus intermedius group (SIG) isolates from dogs in a center for veterinary diagnostics in Brazil / Cassetes cromossômicos estafilocócicos mec de isolados do grupo Staphylococcus intermedius (GSI) de cães em um centro veterinário de diagnósticos no Brasil

ABSTRACT: Methicillin resistance in the Staphylococcus intermedius group (SIG) has emerged in small animal practice. Methicillin-resistant SIG (MRSIG) members have been implicated as causes of infections in both companion animals and humans. Staphylococcal cassette chromosome mec (SCCmec) elements carry the mecA/C genes, which encode for the transpeptidase PBP2a (PBP2') responsible for β-lactam antibiotic resistance in staphylococci. This study examined the SCCmec types of MRSIG isolates from different clinical specimens of dogs that exhibited methicillin MIC ≥ 0.5 μg/mL by an automated identification and susceptibility system in a Center for Veterinary Diagnostics in São Paulo, Brazil. Susceptibility to methicillin was determined by broth microdilution testing, and Oxoid® M.I.C.Evaluator® strips. PBP2a production was detected using a latex agglutination assay. SCCmec typing was performed according to the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC) guidelines. SCCmec type II (2A), SCCmec type III (3A), composite SCC structures consisting of a class A mec gene complex in addition to multiple ccr gene complexes, and non-typable SCCmec elements were reported in these MRSIG isolates. SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates.

RESUMO: A resistência à meticilina no grupo Staphylococcus intermedius (GSI) tem aumentado na clínica de pequenos animais. Membros GSI resistentes à meticilina (GSIRM) têm sido causas de infecções tanto em animais de companhia e humanos. Cassetes cromossômicos estafilocócicos mec (SCCmec) carregam os genes mecA/C, que codificam a transpeptidase PBP2a (PBP2') responsável pela resistência aos antibióticos β-lactâmicos em estafilococos. Nosso objetivo foi investigar os elementos SCCmec de GSIRM isolados de diferentes amostras clínicas de cães que exibiram CIM de meticilina ≥ 0,5 μg/mL por meio de um sistema automatizado em um Centro Veterinário de Diagnósticos em São Paulo, Brasil. A sensibilidade à meticilina foi determinada por meio do teste de microdiluição em caldo e fitas Oxoid® M.I.C.Evaluator®. A produção de PBP2a foi detectada usando um ensaio de aglutinação de látex. A tipagem dos elementos SCCmec foi realizada de acordo com as diretrizes do International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC). SCCmec tipo II (2A), SCCmec tipo III (3A), SCC compostos de um complexo mec de classe A com múltiplos complexos ccr, e elementos SCCmec não tipáveis foram encontrados nesses isolados GSIRM. Variantes que diferem dos elementos SCCmec reconhecidos até o momento pelo IWG-SCC foram encontradas, indicando novos rearranjos no contexto genético de mecA nesses isolados GSIRM caninos.

Phosphorylation residue T175 in RsbR protein is required for efficient induction of sigma B factor and survival of Listeria monocytogenes under acidic stress / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Listeria monocytogenes is an important zoonotic foodborne pathogen that can tolerate a number of environmental stresses. RsbR, an upstream regulator of the sigma B (SigB) factor, is thought to sense environmental challenges and trigger the SigB pathway. In Bacillus subtilis, two phosphorylation sites in RsbR are involved in activating the SigB pathway and a feedback mechanism, respectively. In this study, the role of RsbR in L. monocytogenes under mild and severe stresses was investigated. Strains with genetic deletion (ΔrsbR), complementation (C-ΔrsbR), and phosphorylation site mutations in the rsbR (RsbR-T175A, RsbR-T209A, and RsbR-T175A-T209A) were constructed to evaluate the roles of these RsbR sequences in listerial growth and survival. SigB was examined at the transcriptional and translational levels. Deletion of rsbR reduced listerial growxth and survival in response to acidic stress. Substitution of the phosphorylation residue RsbR-T175A disabled RsbR complementation, while RsbR-T209A significantly upregulated SigB expression and listerial survival. Our results provide clear evidence that two phosphorylation sites of RsbR are functional in L. monocytogenes under acidic conditions, similar to the situation in B. subtilis.

Phosphorylation residue T175 in RsbR protein is required for efficient induction of sigma B factor and survival of Listeria monocytogenes under acidic stress / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Listeria monocytogenes is an important zoonotic foodborne pathogen that can tolerate a number of environmental stresses. RsbR, an upstream regulator of the sigma B (SigB) factor, is thought to sense environmental challenges and trigger the SigB pathway. In Bacillus subtilis, two phosphorylation sites in RsbR are involved in activating the SigB pathway and a feedback mechanism, respectively. In this study, the role of RsbR in L. monocytogenes under mild and severe stresses was investigated. Strains with genetic deletion (ΔrsbR), complementation (C-ΔrsbR), and phosphorylation site mutations in the rsbR (RsbR-T175A, RsbR-T209A, and RsbR-T175A-T209A) were constructed to evaluate the roles of these RsbR sequences in listerial growth and survival. SigB was examined at the transcriptional and translational levels. Deletion of rsbR reduced listerial growxth and survival in response to acidic stress. Substitution of the phosphorylation residue RsbR-T175A disabled RsbR complementation, while RsbR-T209A significantly upregulated SigB expression and listerial survival. Our results provide clear evidence that two phosphorylation sites of RsbR are functional in L. monocytogenes under acidic conditions, similar to the situation in B. subtilis.

METODOLOGÍA BASADA EN SIG Y MODELAMIENTO PARA LA RECOLECCIÓN DE LLANTAS USADAS EN BOGOTÁ / METHODOLOGY BASED ON GEOGRAPHICAL INFORMATION SYSTEM -GIS- AND MODELING FOR THE COLLECTION OF USED TIRES IN BOGOTÁ

En este artículo se presenta una metodología para el estudio del incremento de llantas usadas y su recolección. Se comienza desde el modelamiento con dinámica de sistemas para visualizar el crecimiento del parque automotor y estimar la generación de llantas usadas en la ciudad de Bogotá. Posteriormente se diseña un Sistema de Información Geográfica ­SIG­ con los puntos críticos de generación de llantas usadas, en donde se georreferencia también la malla vial de la ciudad y una ruta de recolección de estos residuos especiales. El SIG muestra los puntos críticos, los puntos de recolección y los potenciales puntos para ampliar la cobertura de rutas en diferentes localidades de la ciudad. Finalmente se determinan las rutas de recolección en las zonas donde se presenta la mayor cantidad de puntos críticos sin atender, siendo estos: Engativá, Bosa y Barrios Unidos. De esta forma se pretende aportar al diseño de rutas para la recolección y manejo de llantas usadas en Bogotá y otras ciudades con similar problemática.

This paper presents a methodology for the study of the increase of used tires generation and their collection. It starts from the modeling with systems dynamics to visualize the growth of the vehicle fleet and estimate the generation of used tires in the city of Bogotá. Subsequently, a Geographic Information System -GIS- was designed with the critical points of generation of used tires, where the city vehicle fleet and a collection route for this special waste were georeferenced. The GIS shows the critical points, the collection points and the potential points to expand the collection routes in different locations in the city of Bogota. Finally, the collection routes are determined in the areas where the greatest number of critical points without attention are present which are Engativá, Bosa and Barrios Unidos. In this way the intention is to contribute to the design of routes for the collection and management of used tires in Bogotá and other cities with similar problems.

Sodium aescinate inhibits proliferation of hepatocellular carcinoma through CARMA3/NF-κB signaling pathway / 中国药理学通报

Aim To investigate the expression of CAR-MA3, NF-κB in hepatocellular carcinoma tissues and the underlying mechanism of sodium aescinate in inhib-iting the proliferation of human hepatocellular carcino-ma cells. Methods The expression of CARMA3 and NF-κB in HCC tissues were detected by tissue microar-ray immunohistochemistry. MTT was used to determine the effect of sodium aescinate on the proliferation of HCC cells. Cell apoptosis was detected by flow cytom-etry. The expression of CARMA3, NF-κB protein in HepG2 and Hep3B cells treated with sodium aescinate was detected by Western blot and cell immunofluores-cence. Results Tissue microarray analysis showed that the expression of CARMA3 in HCC was up-regulated compared with the adjacent adjacent liver tissues, and the histopathological differentiation, TNM stage, tumor volume and prognosis were correlated. Sodium aesci-nate in 40 μmol·L-1concentration ( IC50) inhibited the growth of HCC cell lines, promoting its apoptosis, but without toxic effects on normal liver cells. Western blot and cell immunofluorescence detection of sodium aescinate could significantly inhibit the expression of CARMA3 and NF-κB. Conclusion Sodium aescinate can effectively inhibit the proliferation of HCC cells by inhibiting the activation of CARMA3/NF-κB signaling in HCC.

Effect of sitagliptin on cardiomyocyte pyroptosis induced by type 2 diabe-tes mellitus and underlying mechanism / 中国病理生理杂志

AIM:To explore the effect of sitagliptin (SLT) on cardiomyocyte pyroptosis induced by type 2 dia-betes mellitus (T2DM) and the underlying mechanism. METHODS:The T2DM rat model was established by high-fat diet and intraperitoneal injection of streptozotocin (35 mg/kg). The model rats were treated with SLT at 3, 10 and 30 mg/kg and nicotinamide [NAM; an non-specific inhibitor of sirtuin (SIRT) family] at 500 mg/kg for 4 weeks. Fasting blood glu-cose was measured, and the tissue proteins were determined by the methods of Western blot and immunochemistry. RE-SULTS:Compared with control group, the pyroptosis of cardiomyocytes and NLRP3 expression were significantly induced, while the protein level of SIRT3 was downregulated by T2DM (P<0.05). SLT inhibited the pyrpotosis of diabetic rat car-diomyocytes, downregulated the expression of NLRP3, and upregulated the expression of SIRT3 in a dose-dependent man-ner (P<0.05). All the function of SLT (30 mg/kg) was reversed by the treatment with NAM (500 mg/kg). Compared with control group, the pyroptosis of cardiomyocytes and NLRP3 expression were significantly induced, while the protein level of SIRT3 was not regulated by NAM (500 mg/kg). CONCLUSION:SLT exerts the inhibitory effect on the pyropto-sis of cardiomyocytes induced by diabetes, and the mechanism is related to the SIRT3/NLRP3 signaling pathway.

Role of JAK2/STAT3 signaling pathway in ginsenoside Re pretreatment in prevention of isoproterenol-induced acute myocardial ischemia / 中国药理学通报

Aim To observe the protective effect of ginsenoside Re pretreatment on rats with isoproterenol-induced acute myocardial ischemia via JAK 2/STAT3 signaling pathway .Methods SD rat model with acute myocardial ischemia was established using isoprotere-nol.Seventy-five rats were randomly divided into five groups: control group, model group , puerarin group (PUE), high dose group (Re-H, 20 mg· kg -1) and Re low-dose group ( Re-L, 10 mg kg -1 ) .The blood flow on the heart surface of rats in each group was ob-served by moor laser blood flow imaging system .The levels of CK , LDH, SOD, MDA and GSH in myocar-dium were measured by ELISA .The expressions of Bax and Bcl-2 proteins were detected by immunohistochem-istry.The expressions of JAK , p-JAK, STAT3 and p-STAT3 proteins were detected by Western blot .Re-sults Compared with the control group , the mean blood flow on the heart surface of rats in the model group significantly decreased , the levels of CK , LDH and MDA in the myocardium increased , the levels of GSH and SOD decreased , the ratio of Bcl-2/Bax de-creased ( P <0.05 ) , and the expression of JAK 2/STAT3 pathway related proteins was enhanced ( P <0.05 ) . The mean blood flow on the heart surface markedly increased , the levels of CK , LDH and MDA decreased , the level of GSH-Px increased , the ratio of Bcl-2/Bax increased, and the expression of JAK2/STAT3 pathway proteins evidently increased in the Re-H group compared with those of the model group ( P<0.05 ) .Conclusion Ginsenoside Re pretreatment has a good protective effect on the myocardium in rats with acute myocardial ischemia , which may be related to the activation of JAK2/STAT3 signaling pathway .

Uso de sistemas de información geográfica (SIG) en la valoración del potencial piscícola a nivel municipal / Use of geographic information systems (GIS) for the assessment of fish farming potential at the municipal level / Uso dos sistemas de informação geográfica (SIG) na valoração do potencial para piscicultura ao nível municipal

Resumen La aplicación de los Sistemas de Información Geográfica (SIG) en diversas áreas del conocimiento ha experimentado un gran desarrollo en las últimas dos décadas, especialmente para resolver problemas en la toma de decisiones y la organización de un territorio determinado. Es así como los SIG en piscicultura, han sido especialmente utilizados en la determinación del potencial de cualquier área para el desarrollo de sistemas de cultivo. Además, sirven como una herramienta para dar recomendaciones a los procesos de ordenación y planificación de la actividad. El municipio Castilla La Nueva, ubicado en el departamento del Meta, se seleccionó como área de estudio porque allí la piscicultura constituye un renglón económico importante dentro del sector agrícola. Debido a los efectos ambientales adversos que la piscicultura puede producir (contaminación de agua, cambios de usos del suelo, conflictos de usos, etc.) cuando se realiza sin planificación y orden, se hace necesario introducir técnicas modernas en el análisis de información, para contribuir con la planificación municipal e integrar de forma coherente la piscicultura con el entorno y otras actividades presentes. Los resultados obtenidos con la aplicación del SIG basado en el modelamiento matemático-estadístico están representados por cartografía temática y un mapa de aptitud piscícola de la zona de estudio. Se espera que dichos resultados contribuyan a la identificación de los lugares más aptos para el desarrollo de la piscicultura en Castilla La Nueva, para que así los planificadores del municipio tengan bases adecuadas para organizar el sector rural con mayor conocimiento y compromiso ambiental. También que este sea un ejemplo a seguir para el ordenamiento territorial a nivel de municipios en Colombia.

Abstract There has been a great deal of development in the use of geographic information systems (GIS) in a variety of areas during the past two decades, particularly for decision-making related to regional planning. In fish farming, GIS have been used par- ticularly to determine site potential. Furthermore, they can be used as a tool to provide recommendations for the planning and implementation stages of fish farming projects. The Castilla La Nueva municipality, located in the Meta department in Colombia, was chosen for this study because fish farming activities are an important economic portion of the agriculture sector. Due to the adverse environmental effects, that fish farming may produce when not properly implemented (water pollution, land-use change, land-use conflicts, etc.), the modern information analysis techniques becomes critical for municipal planning tasks such as the integration of fish farming and environmental conservation. The results obtained after implementing the GIS, which was based on mathematical and statistical modeling, are represented by thematic mapping and a map of the studied zone displaying the area's suitability for fish farming. We expect that these results will contribute to the identification of the most suitable places for fish farming in Castilla La Nueva. This will provide the municipality's town planners with the elements required to organize the rural sector with more knowledge and environmental commitment. Likewise, we also hope that this will set an example to be followed by other Colombian municipalities in terms of zoning.

Resumo O uso dos Sistemas de Informação Geográfica (SIG) a diversas áreas do conhecimento tem experimentado um grande desenvolvimento nas últimas dois décadas, especialmente para resolver problemas na toma de decisões e a organização de um determinado território. É assim como os SIG em piscicultura, tem sido especialmente utilizados na determinação do potencial de qualquer área para o desenvolvimento de sistemas de cultivo. Além disso, servem como uma ferramenta para dar recomendações aos processos de ordenamento e planejamento da atividade. O município Castilla La Nueva, lo calizado no departamento do Meta, foi selecionado como área de estudo porque ali a piscicultura se constitui numa parte económica importante dentro do setor agrícola. Devido aos efeitos ambientais adversos que a piscicultura pode produzir (poluição da água, câmbios de usos do solo, conflitos de usos, etc.) quando se realiza sem planejamento nem ordem, é necessário introduzir técnicas modernas na análise de informação, para contribuir com a planificação do município e inte grar de forma coerente a piscicultura com o entorno e outras atividades presentes. Os resultados obtidos com a aplicação do SIG baseado na modelagem matemático-estatístico estão representados pela cartografia temática e um mapa de atitude piscícola da zona de estúdio. Espera-se que ditos resultados contribuam à identificação dos locais mais aptos para o desen volvimento da piscicultura em Castilla La Nueva, para que assim os planejadores do município tenham suporte adequado para organizar o setor rural com maior conhecimento e compromisso ambiental. Também que este seja um exemplo para ser seguido no ordenamento territorial ao nível de municípios na Colômbia.

Geospatial distribution and risk factors for bovine cysticercosis in the state of Rondônia, Brazil / Distribuição espacial e fatores de risco para a cisticercose bovina no Estado de Rondônia, Brasil

This study focused on assessing the prevalence, geospatial distribution and risk factors for bovine cysticercosis in cattle from the state of Rondônia, Brazil, through the years 2012 to 2015. The prevalence established was 0.014% (95% C.I. 0.013-0.014), with a higher detection of unviable cysticerci (84.80%). The municipalities of Itapuã do Oeste, Candeias do Jamari, Nova Brasilândia D'Oeste, Pimenteiras do Oeste, Porto Velho, Nova Mamoré, Urupá and Guajará-Mirim had higher risk (OR>1; p<0.05) for cysticercosis occurrence compared with the municipality of Castanheiras (OR=1). The Administrative Regions of Porto Velho, Guajará-Mirim, Colorado D'Oeste, Cacoal, Ji-Paraná had higher risk (OR>1; p<0.05) for cysticercosis occurrence in the slaughtered animals than those reared in Ariquemes Administrative Region (OR=1). Some variables such as human population density (OR=2.15; 2.15-2.16), percentage of urban houses with inappropriate sewage system (OR=1.91, 1.91-.1.92) and percentage of inappropriate rural sewage system (OR=1.14, 1.14-1.14) were significantly associated (p<0.05) with the occurrence of bovine cysticercosis. In conclusion, the prevalence of bovine cysticercosis in the state of Rondônia was 0.014% (95% C.I. 0.013-0.014) and higher-risk areas were identified, providing useful information to Official Sanitary Inspection System in order to improve cysticercosis detection. Also, human population density and the lack of appropriate sewage system in urban and rural areas are closely related to bovine cysticercosis occurrence in this state.(AU)

Este estudo objetivou avaliar a prevalência, distribuição espacial e fatores de risco para a ocorrência de cisticercose bovina em animais abatidos oriundos do Estado de Rondônia, Brasil, entre os anos de 2012 a 2015. A prevalência observada foi de 0,014% (I.C. 95% 0,013-0,014), com maior detecção de cisticercos inviáveis (84,80%). Os municípios de Itapuã do Oeste, Candeias do Jamari, Nova Brasilândia D'Oeste, Pimenteiras do Oeste, Porto Velho, Nova Mamoré, Urupá e Guajará-Mirim apresentaram maior risco (OR>1; p<0.05) para a ocorrência da enfermidade quando comparadas ao município de Castanheiras (OR=1). As Regiões Administrativas de Porto Velho, Guajará-Mirim, Colorado D'Oeste, Cacoal, Ji-Paraná, respectivamente, apresentaram maior risco (OR>1; p<0.05) para a ocorrência de cisticercose nos bovinos abatidos comparadas à Região Administrativa de Ariquemes (OR=1). Algumas variáveis como a densidade populacional humana (OR=2,15; 2,15-2,16), percentual de domicílios urbanos com saneamento inadequado (OR=1,91, 1,91-1,92) e percentual de saneamento rural inadequado (OR=1,14, 1,14-1,14) apresentaram-se como fatores de risco significativos (p<0.05) para a ocorrência de cisticercose bovina. Conclui-se que a prevalência de cisticercose bovina no Estado de Rondônia foi 0,014% (I.C. 95% 0,013-0,014) e que áreas de maior ocorrência puderam ser identificadas, fornecendo informações a serem utilizadas pelo Serviço de Inspeção Federal para melhorar a detecção dos casos. Ainda, a densidade da população humana e a deficiência de sistemas de tratamento de esgotos urbanos e rurais estão intimamente relacionadas com a ocorrência da cisticercose bovina neste estado.(AU)

Effects of Anisodine Hydrobromide on Cell Apoptosis and ERK1/2 Phosphorylation Level in Brain Tissue of Model Rats with Acute Cerebral Ischemia-reperfusion Injury / 中国药房

OBJECTIVE:To study the effects of anisodine hydrobromide on cell apoptosis and extracellular signal-regulated pro-tein kinase 1/2 (ERK1/2) phosphorylation (p-ERK1/2) level in brain tissue of model rats with acute cerebral ischemia-reperfusion injury. METHODS:Rats were randomly divided into sham operation group,model group,positive control group(nimodipine 1.0 mg/kg),anisodine hydrobromide high-dose,medium-dose,low-dose,extreme low-dose groups(1.2,0.6,0.3,0.15 mg/kg),8 in each group. Suture method was used to establish the rat models with acute cerebral ischemia-reperfusion injury. Rats were intrave-nously injected once in tail at 2nd of ischemia and 6th of reperfusion. Then adenosine triphosphate (ATP) enzyme activity,Ca2+content,cell apoptosis in brain tissue,p-ERK1/2 protein expression in brain tissue,and p-ERK1/2/total ERK1/2 (t-ERK1/2) pro-portion in brain tissue of rats were detected after 22 h of reperfusion. RESULTS:Compared with sham operation group,ATP en-zyme activity in brain tissue of rats in model group was obviously decreased,Ca2+ content was obviously increased,density of cell apoptosis in brain tissue was obviously increased,with statistical significances(P<0.01). Compared with model group,density of cell apoptosis in brain tissue was obviously decreased in each administration group;Ca2+ contents in brain tissue of rats in positive control group,anisodine hydrobromide high-dose,low-dose groups were obviously decreased;and p-ERK1/2/t-ERK1/2 proportion in brain tissue of rats in anisodine hydrobromide high-dose,low-dose,extreme low-dose groups were obviously increased,with sta-tistical significances(P<0.05 or P<0.01);the other differences were not statistically significant(P>0.05). CONCLUSIONS:An-isodine hydrobromide can inhibit the cell apoptosis in brain tissue of model rats with acute cerebral ischemia-reperfusion injury,andthe mechanism may be related with activating ERK1/2 signal pathway and regulating ATP enzyme activity to decrease the Ca2+content in the brain tissue.

Effects of Anisodine Hydrobromide on Cell Apoptosis and ERK1/2 Phosphorylation Level in Brain Tissue of Model Rats with Acute Cerebral Ischemia-reperfusion Injury / 中国药房

OBJECTIVE:To study the effects of anisodine hydrobromide on cell apoptosis and extracellular signal-regulated pro-tein kinase 1/2 (ERK1/2) phosphorylation (p-ERK1/2) level in brain tissue of model rats with acute cerebral ischemia-reperfusion injury. METHODS:Rats were randomly divided into sham operation group,model group,positive control group(nimodipine 1.0 mg/kg),anisodine hydrobromide high-dose,medium-dose,low-dose,extreme low-dose groups(1.2,0.6,0.3,0.15 mg/kg),8 in each group. Suture method was used to establish the rat models with acute cerebral ischemia-reperfusion injury. Rats were intrave-nously injected once in tail at 2nd of ischemia and 6th of reperfusion. Then adenosine triphosphate (ATP) enzyme activity,Ca2+content,cell apoptosis in brain tissue,p-ERK1/2 protein expression in brain tissue,and p-ERK1/2/total ERK1/2 (t-ERK1/2) pro-portion in brain tissue of rats were detected after 22 h of reperfusion. RESULTS:Compared with sham operation group,ATP en-zyme activity in brain tissue of rats in model group was obviously decreased,Ca2+ content was obviously increased,density of cell apoptosis in brain tissue was obviously increased,with statistical significances(P<0.01). Compared with model group,density of cell apoptosis in brain tissue was obviously decreased in each administration group;Ca2+ contents in brain tissue of rats in positive control group,anisodine hydrobromide high-dose,low-dose groups were obviously decreased;and p-ERK1/2/t-ERK1/2 proportion in brain tissue of rats in anisodine hydrobromide high-dose,low-dose,extreme low-dose groups were obviously increased,with sta-tistical significances(P<0.05 or P<0.01);the other differences were not statistically significant(P>0.05). CONCLUSIONS:An-isodine hydrobromide can inhibit the cell apoptosis in brain tissue of model rats with acute cerebral ischemia-reperfusion injury,andthe mechanism may be related with activating ERK1/2 signal pathway and regulating ATP enzyme activity to decrease the Ca2+content in the brain tissue.

DPP-4 inhibitor Sig Leo Dean on the newly diagnosed type 2 diabetes mellitus and its effect on blood pressure and body weigh / 重庆医学

Objective To investigate the clinical efficacy of DPP-4 inhibitors in the treatment of newly diagnosed type 2 diabetes mellitus and its effect on blood pressure and body weight.Methods A total of 100 newly diagnosed elderly patients with type 2 diabetes mellitus were enrolled in this study from July 2013 to June 2015 in this hospital.They were divided into observation group and control group(n=50).The control group was treated with placebo on the basis of diet control and exercise.The observation group was treated with sitagliptin on the basis of diet control and exercise.Fasting plasma glucose(FPG),2 h postprandial blood glucose(2 h PG) and glycated albumin (GA) were measured before and after treatment,and the incidence rate of hypoglycemia,blood pressure,body weight and other adverse reactions were monitored.Results After treatment,two groups of patients,condition were significantly improved,FPG,2 h PG,GA changes in observation group were significantly better than that of control group,the difference were statistically significant(P＜0.05).There was no significant difference in blood pressure,body weight and body mass index before and after treatment,and also between the two groups(P＞0.05).The incidence of adverse reactions in the observation group and the control group were 10.0％ and 8.0％,The incidence of adverse reactions in the two groups was not statistically significant(P＞0.05).Conclusion The clinical efficacy of DPP-4 inhibitor siglitazide in the treatment of newly diagnosed elderly patients with type 2 diabetes mellitus is good,and it is very useful in the treatment of these patients.

Effect of gliclazide and sitagliptin on insulin control in poorly controlled obese patients with type 2 diabetes mellitus / 中国生化药物杂志

Objective To explore the effect of gliclazide and Sig Leo Dean on insulin treatment for poor glycemic control in obese patients with type 2 diabetes mellitus. Methods 80 cases of insulin treatment for poor glycemic control in obese patients with type 2 diabetes mellitus treated in our hospital from March 2015 to January 2017 were randomly divided into two groups,with 40 cases in each group.The control group was treated with gliclazide The observation group was treated with Sig Leo Dean.The therapeutic effects of the two groups were observed and compared. Results 2 hours postprandial blood glucose levels improved in the observation group was better than that of the control group, the body weight, blood pressure, fasting blood glucose of two groups were improved, and there were no significant difference between the two groups; The incidence rate of adverse reactions was 2.5% in the observation group, 10% in the control group, the difference was not statistically significant (P<0.05). Conclusion Analysis of gliclazide and Sig Leo Dean on insulin treatment for poor glycemic control in obese patients with type 2 diabetes mellitus curative effect, Sig Leo Dean for the glycaemic improvement effect is higher, reduce the incidence of adverse reactions, so more medication safety, it is worthy of clinical promotion Application.

Cambios en la cobertura de manglares en Bahía Culebra, Pacífico Norte de Costa Rica (1945-2010) / Changes in mangrove coverage in Culebra Bay, North Pacific of Costa Rica (1945-2010)

ResumenLos manglares tienen gran importancia ecológica, económica, riqueza natural y prestan servicios ambientales. No obstante, son amenazados por la sobreexplotación, la contaminación y el cambio de uso de suelo. Costa Rica tiene manglares en las costas pacífica y caribeña. Según algunos estudios, la cobertura de manglar ha ido disminuyendo desde la década de 1980. Debido a que estos datos no son actualizados y se basan en estimaciones poco precisas, es necesario hacer una valoración de la extensión actual y la variación de la cobertura en los últimos años, que permita determinar cambios. En esta investigación se estudió la cobertura de dos manglares ubicados en Bahía Culebra, Pacífico Norte: Iguanita y Playa Panamá. Se usaron fotografías e imágenes de satélite para un período de 65 años (1945-2010). Se encontraron cambios espaciotemporales en la cobertura de manglar, bosques adyacentes y áreas sin vegetación. Las menores coberturas de manglar se registraron en la década de 1970, pero aumentaron en años posteriores. Los cambios en la cobertura de bosque alrededor de los manglares de Iguanita y Playa Panamá concuerdan con otros análisis históricos sobre el uso del suelo alrededor de Bahía Culebra. Antes de 1980 se dio un aumento de las prácticas de ganadería extensiva e intensiva, lo que aumentó la tasa de deforestación. Después de 1980 se abandonaron estas prácticas y la cobertura de bosque secundario aumentó hasta el año 2000. Para asegurar una adecuada protección de los manglares, es necesario evaluar también las áreas aledañas y establecer zonas de amortiguamiento alrededor, para reducir los impactos futuros.

Abstract:Despite the economic and environmental services that mangroves provide, they continue to be threatened by overexploitation, pollution, and land use change. Costa Rica has mangrove areas on the Pacific and Caribbean coasts, and cover has been declining since the 1980s. However, data on mangrove coverage are not continually updated and are often based on inaccurate estimates. It is therefore necessary to assess the current extension and variation of the mangrove cover in recent years, to determine changes. The mangrove cover was analyzed in two mangrove forests located in Bahía Culebra, North Pacific: Iguanita and Playa Panamá. For this, aerial photographs and satellite imagery were used to study changes for a 65 year period (1945-2010). Spatio-temporal changes were found in mangrove coverage, adjacent forests and areas without vegetation. Lower mangrove cover occurred during the 1970s (28.4 ha in Iguanita and 4.8 ha in Playa Panamá); but increased in recent years (38.9 ha in Iguanita and 12.0 ha in Panamá). Changes in forest cover by the Iguanita and Playa Panama mangroves were related to the history of land use around Bahía Culebra. Before 1980, there was extensive and intensive cattle ranching, increasing the deforestation rate; after that year, these practices were abandoned and secondary forest coverage increased until 2000. To ensure the adequate protection of mangroves, it is not only important to protect mangrove forests, but it is also necessary to establish buffer zones on their surroundings, to mitigate and/or reduce possible impacts. Rev. Biol. Trop. 64 (3): 955-964. Epub 2016 September 01.

VARIACIÓN DE COBERTURAS FORESTALES Y OCUPACIÓN DEL TERRITORIO EN EL MUNICIPIO DE ARMENIA 1939-1999 / VARIATION OF FOREST COVERAGE AND OCCUPATION OF THE LAND IN THE MUNICIPALITY OF ARMENIA 1939-1999

Introducción: Al observar la variación de la cobertura vegetal en un territorio, se puede observar su historia escrita sobre ese paisaje. Objetivo: El presente artículo pretende describir la variación de coberturas vegetales en relación con la dinámica de ocupación y poblamiento de la ciudad de Armenia, desde el año 1939 hasta el año 1999. Método: La serie de aerofotografías del municipio de Armenia existentes en el IGAC, desde 1939 hasta 1999, se georreferenciaron, se construyeron los mosaicos para cada uno de los años, se calcularon las áreas a través de una herramienta SIG (Sistema de Información Geográfica) y se determinaron coberturas vegetales y áreas pobladas. Se comparó la variación año a año con la dinámica de ocupación y el poblamiento del territorio. Resultados: El área urbanizada creció de 132,9 ha en 1939, a 1.316,23 ha en 1999. El área en coberturas vegetales creció de 82,52 ha que representaban el 38,32% del área poblada en 1939, hasta 1.048 ha que representaban el 45,35% del perímetro urbano en 1999. Conclusión: El porcentaje de cobertura vegetal aumentó y ahora se considera suelo de protección, lo cual confiere especiales características al paisaje de Armenia, que debe ser protegido y conservado.

Introduction: By observing the variation of the vegetation coverage in an area, it is possible to see its history written on the landscape. Objective: This article aims to describe the variation of vegetation coverage in relation to the dynamics of occupation and settlement of the city of Armenia, from 1939 until 1999. Method: The series of aerial photographs (by Agustin Codazzi Geographical Institute IGAC) of the city of Armenia from 1939 to 1999 were georeferenced and mosaics for each of the years were assembled by calculating the areas through a GIS tool. Vegetation coverage and urban land use were determined. The variation year to year was compared with the dynamics of occupation and settlement of the territory. Results: The urbanized area has increased from 132.9 hectares in 1939 to 1316.23 hectares in 1999. The vegetation coverage area rose from 82,52 hectares which represented 38.32% of the populated area in 1939, to 1,048 hectares, representing 4535% of the city limits in 1999. Conclusion: The percentage of vegetation coverage increased and is now considered protected land which gives special characteristics to the landscape of Armenia that must be protected and conserved.