RESUMO
Leukemia is a polygenic malignant proliferative disease in the hematopoietic system.Its possible causative genes and gene therapy are one hotspot of the current researches.Sex-determining region Y-related high-mobility-group box transcription factor 4(SOX4) is a member of the group C subfamily of the SOX transcription factors,playing a critical role in the occurrence and development of many tumors such as lung cancer,breast cancer,hepatic carcinoma,gastric cancer and medulloblastoma.Recently,researches have found that SOX4 is significant in the occurrence,treatment and prognosis evaluation of leukemia.This paper reviews the structure and functions of SOX4,its expression and mechanism in leukemia,and its influence on leukemia treatment and prognosis.
RESUMO
Recent studies have revealed that sox4 gene expresses abnormally in many kinds of tumor tissues and it probably involves in tumorigenesis,development and metastasis of cancer.Regulating proliferation,differentiation,apoptosis and other process may participate in the work mechanisms of sox4 gene.Therefore,further studies about the relationship between sox4 gene and tumor would provide new ideas of exploring special diagnosis markers and novel targets for tumor therapy.
RESUMO
Objective To explore the role of menin in regulation of SOX4 gene expression. Methods Reporter gene vectors with SOX4 promoter were constructed,and the influence of menin on SOX4 gene promoter activity was analyzed by dual-luciferase reporter gene assay system.Real-time PCR was employed to detect the expression of SOX4 gene in mef cells of MEN1-/-mice and wild-type mef cells. Results Compared with wild-type mef cells,the SOX4 gene promoter activity was significantly higher in mef cells of MEN1-/-mice.After MEN1 gene was transfected into mef cells of MEN1-/-mice,the SOX4 gene promoter activity significantly descreased.The expression of SOX4 gene in mef cells of MEN1-/-mice was 2.5 time higher than that in wild-type mef cells. Conclusion Menin can inhibit the expression of SOX4 gene.