Study on chemical constituents and in vitro antioxidant activity of ethanol extract of Sabia parviflora / 中国药房

OBJECTIVE To separate and identif y the chemical constituen ts in 70％ ethanol extract of Sabia parviflora ，and to preliminarily evaluate their in vitro antioxidant activity. METHODS The chemical constituents were separated and purified by silica gel，ODS reversed-phase silica gel ，Sephadex-LH20 column and preparative high performance liquid chromatography. The structures of the isolated compounds were identified by 1H-NMR，13C-NMR and ESI-MS. The in vitro antioxidant activities of the compounds were investigated by 2，2-diphenyl-1-picrylhydrazyl radical （DPPH·），2，2′-azino-bis（3-ethylbenzothiazoline-6- sulfonate）diammonium radical （ABST+）and hydroxyl radical （OH·）. RESULTS A total of 9 compounds were isolated from the 70％ ethanol extracts of S. parviflora . They were identified as rutin （1），diiononyl phthalate （2），dibutyl phthalate （3），vomifoliol （4），rhododendrol（5），quercetin-3-O-gentiobioside（6），narcissoside（7），kaempferol-3-O-rutinoside（8）and bonaroside （9）. The in vitro antioxidant results showed that compound 1-9 showed certain in vitro antioxidant activity ，and the half scavenging concentrations of compound 1，6，7 and 8 to DPPH ·，ABST+，OH·were lower than 70 μg/mL. CONCLUSIONS Vomifoliol， rhododendrol and bonaroside are isolated from S. parviflora for the first time ，and rutin ，quercetin-3-O-gentiobioside，narcissoside and kaempferol- 3-O-rutinoside show good in vitro antioxidant activity.

Ecology and Quality Suitability Regionalization of Sabia parviflora / 中国实验方剂学杂志

Objective:To explore the potential suitable distribution area and the high-quality distribution area of <italic>Sabia parviflora</italic>. Method:Combined with the distribution information and environmental factors，the maximum entropy （MaxEnt） model and ArcGIS software were used to predict the potential suitable distribution area of <italic>S. parviflora</italic>. Based on the correlation between environmental factors and total saponins，total flavonoids，quercetin-3-<italic>O</italic>-gentiobioside，camellianoside，tsubakioside A，kaempferol-3-<italic>O</italic>-rutinoside and isobariclisin-3-<italic>O</italic>-rutinoside，the quality regionalization was conducted by using spatial interpolation method and fuzzy superposition function in ArcGIS software. Result:<italic>S. parviflora</italic> is mainly distributed in Yunnan，Guizhou，Guangxi province in China. The medium and high suitable areas accounts for about 2.88% of the national area. The precipitation in October and November，the precipitation in the warmest and driest seasons，the standard deviation of seasonal changes in temperature and altitude are the main environmental factors that affect the distribution of <italic>S. parviflora</italic>. Slope，precipitation，solar radiation and temperature change had great influence on the accumulation of secondary metabolites. Based on the results of potential suitable distribution and spatial interpolation of each component，the high-quality areas of <italic>S. parviflora</italic> are mainly concentrated in the southwest of Guizhou，with Qinglong，Guanling，Zhenning，Pu'an，Xingren county and other areas as the core. Conclusion:This study provides a scientific guidance for the site selection of artificial planting and the procurement of medicinal materials for <italic>S. parviflora</italic>.

Study on protective effect of water extract from Sabia parviflora on liver injury in mice induced by acetaminophen / 中国中药杂志

The aim of this study was to observe the protective effect of water extract from Sabia parviflora on mice with acute liver injury induced by acetaminophen, and investigate its possible mechanism. Fifty-eight Kunming mice were divided into 6 groups, 8 in the normal group, 10 in the model group, 10 in the biphenyl diester group, and 10 each in the low, medium and high dose groups. After adaptive feeding for one week, the mice in normal group were intragastrically administered with an equal volume of 0.5% sodium carboxymethylcellulose sodium(CMC-Na), and the mice in other groups were intragastrically administered with corresponding drugs at 20 mL·kg~(-1) once a day. Then acetaminophen(200 mg·kg~(-1)) was administered after the above drug administration except the normal group. The behavior and signs of the experimental animals were observed every day and the samples were taken for experiments on the next day of the final administration. The liver mass and mass index were calculated. The blood was collected from the abdominal aorta and centrifuged to obtain the serum for detecting aspartate aminotransferase(AST) activity and alanine aminotransferase(ALT) activity. The liver tissue homogenate was used to detect superoxide dismutase(SOD) activity, glutathione(glutathione, r-glutamyl cysteingl+glycine, GSH) activity and malondialdehyde(MDA) content. Liver tissue was analyzed for histological analysis. The results showed that S. parviflora could alleviate the lipid peroxidation damage in the liver caused by acetaminophen, reduce the ALT and AST activities in serum, increase the levels of SOD and GSH in liver tissue, decrease the content of MDA in liver tissue, and inhibit the apoptosis. S. parviflora could also improve the live histopathological profile, protect liver cells and restore liver function. Among them, the high dose had the most significant effect and showed dose-effect relationship. This study indicated that S. parviflora had a significant protective effect on acetaminophen-induced liver injury in mice, and its mechanism may be related to its anti-oxidation effect and inhi-bitory effect on apoptosis.

Isolation and identification of chemical constituents from Sabia parviflora (II) / 中草药

Objective: To study the chemical constituents from Sabia parviflora. Methods: Various column chromatographic techniques were used to separate and purify the chemical constituents which structures were elucidated by spectral analysis. Results: Fifteen compounds were isolated and identified as 1,3,5-trimethoxybenzene (1), vanillic aldehyde 4-O-β-D-glucopyranoside (2), 2,6-dimethoxy-4-hydroxybenzyl alcohol 1-O-β-D-glucopyranoside (3), rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo [3,2,1]-oct-8-yl)-3-methyl-2Z,4E-penta-dienoic acid (4), protocatechuic acid (5), 4-quinolinone-2-caboxylic acid (6), 3,4,5-trimethoxy benzoic acid (7), pyrocatechol (8), syringic acid-4-O-β-D-glucopyranoside (9), (-)-(7α,8S)-erythro-1-C-syringylglycerol 4-O-β-D- glucopyranoside (10), 3,4,5-trimethoxy benzoic acid (11), 1,2,4-trihydroxybenzene (12), ferulic acid (13), vanillic acid (14), and p-hydroxybenzoic acid (15). Conclusion: Compounds 1-11 are isolated from the genus of Sabia for the first time, and compounds 12-15 are isolated from this plant for the first time.

Study on HPLC fingerprint of Sabia parviflora / 中国中药杂志

This research was performed to establish the HPLC fingerprint of Sabia parviflora. HPLC method was carried out on a Thermo Accucore-C18(4. 6 mm×150 mm,2. 6 μm) column by 30% tetrahydrofuran in methyl alcohol-acetonitrile-0. 1% phosphate solution as mobile phase at a flow rate of 1. 0 m L·min-1,the column temperature was 30 ℃ and the detection wavelength was 360 nm. The fingerprints were further evaluated by chemometrics methods including similarity analysis,hierarchical clustering analysis,and principal component analysis. In HPLC fingerprint,15 common peaks were selected as the common peaks,and 6 contents of them were identified. The similarity degrees of 38 batches of the samples was more than 0. 710,and the samples were divided into 6 clusters by their quality difference. The method was precision,repeatable,stable,simple and reliable,which could be used for quality control and evaluation of S. parviflora.

Isolation and identification of chemical constituents from Sabia parviflora / 中草药

Objective To study the chemical constituents from the Sabia parviflora. Methods Various column chromatographic techniques were used to separate and purify the chemical constituents which structures were elucidated by spectral analysis. Results Fifteen compounds were isolated and identified as bis (2-ethylhexyl) benzene-1,2-dicarboxylate (1), dibutyl phthalate (2), darutigenol (3), sucrose (4), 3,5-dimethoxy-4-hydroxybenzaldehyde (5), vanillin (6), cleomiscosin C (7), grossamide (8), (-)-lyoniresino (9), ervatamisin (10), (+)-syringaresinol (11), seslignanoccidentaliol A (12), (+)-syringaresinol-4-O-β-D-glucopyranoside (13), (-)-simulanol (14), and (-)-7R,8S-dehydrodiconiferyl alcohol (15). Conclusion All the compounds are isolated from the genus of Sabia for the first time.

Chemical constituents of Sabia parviflora / 中草药

Objective: To investigate the chemical constituents from the stems and leaves of Sabia parviflora. Methods: Column chromatographies over silica gel, Sephadex LH-20, reverse phase C18, MCI, and semi-preparative HPLC were used repeatedly for separation and purification of chemical constituents, and their structures were identified by NMR and MS spectra data with those reported in literature. Results: Fourteen compounds were obtained from the petroleum ether extract of S. parviflora, and identified as β-sitosterol (1), mominine (2), (20S)-3-oxo-20-hydroxytaraxastane (3), fluoren-9-one (4), N-formyldehydroanonain (5), betulinic acid (6), dehydroformouregine (7), palmitic acid (8), 20-hydroxy-lupan-3-one (9), 3-oxooleanolic acid (10), erythrodiol (11), methyl betulinate (12), N-formyl-annonain (Z) (13), and N-formyl-O-methylisopiline (14). Conclusion: Among the 14 compounds, there are seven pentacyclic triterpenes, four alkaloids, and three other compounds; compounds 3-10 and 12-14 are isolated from this plant and the plants of Sabia Colebr. for the first time, and the 13C-NMR spectral assignments of compounds 5, 7, and 14 are reported for the first time.