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Objective To establish a mouse model for scleroderma.Methods Forteen BALB C and 14 C3H female mice were averagely divided into model 1 and controls.Daily 0 1 ml BLM at a concentration of 200 ?g/ml was injected intracutaneusly into the backs of model 1 mice for 3 weeks,and 0 1 ml solution of PBS were injected intracutaneusly into the backs of control mice for 3 weeks.Observing the histological change of skin and lungs was made and measuring the thickness of dermis was performed with a medical analysis system of the color picture,determined the collagenic quantity was done with photoelectric colorimetry,and calculating the immunohistochemical index of collagen type Ⅰ and Ⅲ and transforming growth factor ? 1 (TGF ? 1) in the skin lesions from the mouse model and control was done.SPSS was used to finish the statistical analysis of the detective value from model 1 and controls.Results In the skin of model mice,the thickness of dermis markedly thickened ( P
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Objective Investigation of the effect of integrin on fibroblasts from scleroderma in the production of procollagen. Methods Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin ? 5 or ? 1 subunit on scleroderma fibroblasts respectively, then the changes of procollagen mRNA were detected by RT PCR. Results The expression of integrin ? 5 or ? 1 subunit could be specifically inhibited by their antisense oligonucleotides respectively. Decreased expression of fibroblast integrin ? 5 or ? 1 subunit significantly lowered mRNA of procollagen ? 1(Ⅰ) and ? 1(Ⅲ). Conclusion Overproduction of procollagen may be inhibited in the level of transcription by lowering the expression of integrin on fibroblasts in scleroderma.
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Objective To establish the mouse model of sclerotic skin. Methods The sclerotic skin was induced by local injections of bleomycin (BLM) in C3H and BALB/c mice. The injection solution was prepared with BLM at the concentration of 200?g/mL in PBS. In the test group 0.1 mL BLM solution was injected daily into the back of the mice for 3 weeks. For the control mice same amount of PBS was injected daily for 3 weeks. After 3 weeks, the histology of the skin and lungs was compared between the different groups, also the changes of skin thickness and quantity of collagen. Results After the treatment of BLM sclerotic skin was observed in both C3H and BALB/c mice, in which the thickness of skin and the quantity of collagen (6 mm ? 6 mm) were higher than those in the control mice (P