Chemical constituents and pharmacological activities of Sedum aizoon form Guizhou province / 中草药

Objective: To study the chemical constituents of Sedum aizoon and to screen the anti-oxidant activities and α-glucosidase inhibitory activities of compounds. Methods: The compounds were separated and purified by various chromatographic techniques, and their structures were identified by physio-chemical properties and EI-MS, ESI-MS, 1H and 13C NMR. The anti-oxidant activity of compounds was screened by DPPH method. The obtained compounds were subjected to detection of α-glucosidase inhibitory activity by PNPG method. Results: Seventeen compounds were isolated from S. aizoon, which were identified as iriflophene (1), kaempferol (2), quercetin (3), myricetin (4), rhamnazin-3-O-β-D-glucopyranoside (5), isolariciresinol-9-O-β-D-glucopyranoside (6),myricitrime (7), myricetin-3-O-α-L-arabinopyranoside (8), iriflophenone-2-O-β-D-glucopyranoside (9), 2-O-(trans-caffeoyl)-malic acid 1-methyl-ester (10), 2-O-(trans-caffeoyl)-malic acid 1,4-dimethyl ester (11), 2-O-(trans-caffeoyl) malic acid (12), p-coumaric acid (13), ethyl gallate (14), butanedioic acid (15), 9(Z)-octadece-namide (16), and lotaustralin (17). Conclusion: Compounds 13 and 15 are isolated from S. aizoon for the first time. Compounds 9-12, and 16 are isolated from genus Sedum for the first time. Compounds 2, 3, 7, 8, 10, 12, and 14 had significant anti-oxidant activity. Compounds 8 showed moderate α-glucosidase inhibitory activity in vitro. Compound 3 showed strong α-glucosidase inhibitory activity in vitro.

Mechanism of Therapeutic Effect of Ligustrazine on Hepatic Veno-occlusive Disease Induced by Sedum aizoon in Mice / 胃肠病学

Background:Hepatic veno-occlusive disease( HVOD) is a disease characterized by hepatomegaly,jaundice, ascites,weight gain and lack of effective treatment currently. Our prophase research showed that ligustrazine had therapeutic effect on Sedum aizoon induced HVOD in mice. Aims:To investigate the mechanism of therapeutic effect of ligustrazine on Sedum aizoon induced HVOD in mice. Methods:A total of 115 mice were randomly divided into 4 groups:mice in group A were intragastrically administrated with 30 mg·kg-1 ·d-1 Sedum aizoon to induce HVOD and served as model group;mice in group B were given 30 mg·kg-1 ·d-1 Sedum aizoon + 100 mg·kg-1 ·d-1 ligustrazine and served as low dose ligustrazine intervention group;mice in group C were given 30 mg·kg-1 ·d-1 Sedum aizoon + 200 mg·kg-1 ·d-1 ligustrazine and served as high dose ligustrazine intervention group;mice in group D were given 30 mg·kg-1 ·d-1 PBS and served as normal control group. After 30 days,all the mice were sacrificed. HE staining and Masson staining were performed for histological examination. The mRNA and protein expressions of tissue factor(TF),nuclear factor(NF)-κBp65 and early growth response factor( Egr)-1 in liver tissue were determined by RT-PCR and Western blotting, respectively. Results:HE staining and Masson staining histological examination showed that ligustrazine could obviously ameliorate the pathological injury of liver tissue in HVOD mice. Compared with group D,the mRNA and protein expressions of TF,NF-κBp65,Egr-1 were significantly increased in group A( P 0. 05). Conclusions:Ligustrazine has therapeutic effect on HVOD,the possible mechanism is that ligustrazine could interrupt the activation of coagulation system by reducing the expression of TF via down regulating the expressions of NF-κBp65 and Egr-1,especially in high dose ligustrazine group.

Computed tomography findings of hepatic veno-occlusive disease caused by Sedum aizoon with histopathological correlation

This study investigated the value of computed tomography (CT) in the diagnosis and treatment of hepatic veno-occlusive disease (HVOD) caused by Sedum aizoon (SA). The clinical manifestations, treatment results, imaging findings, and histological findings of the liver were analyzed in 39 patients with HVOD caused by SA. Hepatomegaly, liver dysfunction, abdominal effusion, and geographic density changes on liver CT scans were found in all 39 patients. The pathological findings of histological liver examination included swelling and point-like necrosis of liver cells, significant expansion and congestion of the sinuses, endothelial swelling, and wall thickening with incomplete lumen occlusion of small liver vessels. CT geographic density changes were confirmed by histological examination of the liver in 18 patients. Sixteen patients with small amounts of ascites that started within 4 weeks of treatment recovered completely or significantly improved after symptomatic and supportive treatment. However, only 43.75% of the patients with larger amounts of ascites improved following symptomatic and supportive treatment. In conclusion, liver CT examination is a valuable, safe, and noninvasive tool for the diagnosis of HVOD caused by SA. In selected cases, liver CT examination may replace liver biopsy and histological analysis.

Diagnosis and treatment of hepatic veno-occlusive disease induced by sedum aizoon in HBsAg positive patients / 中华临床感染病杂志

Objective To review the diagnosis and treatment of hepatic veno-occlusive disease(HVOD)induced by sedum aizoon in HBsAg positive patients. Methods Clinical data of 35 HBsAg positive cases who took sedum aizoon decoction and developed HVOD were collected, the clinical manifestation, imaging examination, histological examination of liver puncture biopsy, and the outcomes of patients were reviewed. Results Hepatomegaly, liver dysfunction, abdominal effusion and map-like density changes in liver CT scan were observed in 35 patients. Liver biopsy wag performed in 17 patients. In histopathological examination, the swelling and point-like necrosis of liver cells, expansion and congestion of sinus, endothelial swelling, wall thickening, incomplete lumen occlusion of small liver vascular were observed. Map-like density changes in liver CT scan were found in all 17 patients who were diagnosed by histological examination. Fifteen patients presented small amount of ascites within 4 weeks of onset, 13 of whom recovered or improved after treated with low-molecular weight heparin and albumin; while among the remaining 20 patients. only half of them were benefited from the same treatments. Conclusion HVOD can be diagnosed by liver CT scan instead of histological examination; treatment of patients in early stage may improve the outcome.

Studies on HPLC fingerprint of Herba Sedum aizoon L / 中华中医药杂志

Objective:The fingerprint chromatograms were established for quality evaluation of Sedum aizoon L.collected from different habitats by HPLC.Methods:The analysis was performed on a Diamonsil C18 column(4.6mm? 250mm,5?m)with acetonitrile-water(acidified to 0.5%with phosphoric acid)as mobile phase in a gradient mode at a flow rate of 1.0 mL/min,and at a column temperature of 25℃.The detection of wavelength was at 254 nm.Results:2lpeaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values and relative peak areas were less than 3%in the precision and repeated test.The similarity of l0 batches of samples were no less than 0.9.Conclusion:The method was reliable and can be helpful on the quality control of Sedum aizoon L.

Studies on quality standard of Sedum aizoon L / 中华中医药杂志

Objective: To establish the quatity standard of Sedum aizoon L..Methods: The micro-method and TLC were used for qualitative identification,and a HPLC analysis was applied for quantitative determination of quercetin.Results: A qualitative analysis for Sedum aizoon L.was set up,a good linear relationship was obtained over the range of 1.216-2.16?g/ml and regression equation was Y=40386X-14138(r=0.9991).The average recovery rate was 102.08%(RSD=0.92%).Conclusion: The method is simple,accurate and suitable for quality control of Sedum aizoon L.

Determination and Analysis of Kaempferol in Sedum aizoon L.by HPLC-MS/MS / 中华中医药杂志

Objective: To discover and determine the content of kaempferol in Sedum aizoon L.for the first time.Methods: Waters HPLC-MS/MS,XTerra-MS C18 (5?m,2.1?150mm) and the mobile phase consisted of acetonitrile-water-formic(40∶60∶1) were applied to find kaempferol in Sedum aizoon L.;Daojin HPLC and the SHIM-PACK VP C18(250nm?4.6nm,10?m) column were used.The mobile phase consisted of methanol-0.4% phosphoric acid solutions(59:41) with the flow rate at 1.0ml/min and the UV detector wave-length were set at 370nm.Results: Compared with standard sample,the thing that kaempferol exists in Sedum aizoon L.was confirmed.The calibration curve was in good linearity over the range of 2.0-8.0?g,and regression equation was Y=40343X-11107(r=0.9998).The average recovery rate was 102.53%,with RSD =0.92%(n=6).Conclusion: The method is simple,accurate and reproducible so it can be used to determine and analyze the content of kaempferol in Sedum aizoon L.

Determination of gallic acid and total phenolic acid in Sedum Aizoon L / 中成药

AIM: To develop the methods for the quantitative analysis of gallic acid and total phenolic acid in Sedum Aizoon L. METHODS: Gallic acid was analyzed by HPLC on a Hypersil BDS C_(18) column and detected at 271 nm.The mobile phase was methol-water(adjusted to pH=3.0 with H_3PO_4)(90∶10)at a flow rate of 1.0 mL/min.Total phenolic acid was analyzed by spectrophotometry at 720 nm.The colour-developing agent was the mixture solution of 0.6%FeCl_3—0.9%K_3[Fe(CN)_6](1∶0.9). RESULTS: Calibration graphs were constructed in the range of 0.343 0-1.200 ?g(r=0.999 7) for gallic acid and 0.4640-2.320 ?g/mL(r=(0.999 3)) for total phenolic acid.The average recoveries were 97.91%(n=6) for gallic acid and 99.21%(n=6) for total phenolic acid.The RSD of the methods were 1.8% and 2.0%,respectively. CONCLUSION: The methods were fast,reliable,accurate and suitable for analysis of gallic acid and total phenolic acid and quality control in Sedum Aizoon L.