Serological and molecular investigation of B (A) blood group and its transfusion strategy: A case report / 中国输血杂志

【Objective】 To study the serological and genetic characteristics of a case of B(A) blood group. 【Methods】 Serological and genetic ABO blood group typing were used to analyze the ABO subtype and family inheritance of the probands and her 8 family members. The B(A) blood type sample was used as the blood recipient, and the B-type and O-type donors were selected for cross-matching using microcolumn gel anti-human globulin method to evaluate the blood transfusion strategy. 【Results】 5 out of 9 family blood samples were B(A) phenotype, carrying B(A)04 allele. Among them, 1 was B(A)04/O1 type, and 4 were B(A)04/B type. The primary blood matching of B(A) blood type samples with type B and O recipients were all negative. 【Conclusion】 A total of 5 cases of B(A)04 blood type were found in this family investigation, and there were differences in serological manifestations. Washed RBCs with B and O type can be used for B(A) blood type transfusion, and type B suspended RBCs can be considered in case of emergency.

Two - step PCR - SSP genotyping for HLA - A locus and its comparison with serological typing results / 中国法医学杂志

Objective To identify routine forensic samples by genotyping method for HLA - A locus. Method A two - step PCR - SSP method was established. The first step is amplification with a pair of primers specific for all HLA - A alleles, the second step is amplification with primers specific for HLA - A30, A31, A33 respectively, using the first step amplification product as template. Secondly amplified PCR products were genotyped by electro-phoresis. Results 100 blood stains with a serological typing result of HLA - A30, A31 and A33 were tested with this method. The discrepancy rate between serological and genetic typing was 29%. Seminal stains, salivary stains reserved for 2 years and blood stains reserved for 18 years in room temperature gave satisfactory results. Conclusions It is better to replace serological typing by genetic typing. A two - step PCR - SSP genotyping method can be applied to forensic samples.

Comparison of HLA-Cw Typing by Serology and PCR-SSP in the Korean Population / 대한임상병리학회지

BACKGROUND: Using the classical serological methods, the HLA-Cw typing resulted in a high frequency of Cw blank because of the lack of suitable antisera coupled with low cell surface expression. Recent data on association between graft-versus-host disease and serologically undetectable HLA-Cw mismatches in bone marrow transplantation (BMT) facilitate the investigations into the biological role of HLA-Cw and more reliable HLA-Cw typing. METHODS: We performed the HLA-Cw DNA typing using PCR-SSP technique with sequence-specific primers of 22 pairs in 150 Koreans (79 organ transplant recipients, 71 healthy potential donors). These results were compared with those of serological HLA-Cw typing, which had been performed by complement-dependent microlymphocytotoxicity technique using Terasaki Tissue typing tray. RESULTs: Comparison between serological and PCR-SSP typing revealed a discrepancy rate of 24.0% (36/150). The majority of total discrepancies (29/36, 80.6%) were due to antigens that were not detected serologically and these antigens consisted of mainly Cw*12 and Cw*15. In five cases, no Cw allele was detected by DNA typing, whereas serological typing showed antigens and different antigen assignments between two methods were found in three cases. Of 66 individuals typed serologically with one blank, 66.6% (44 cases) were confirmed to be homozygous, whereas an additional Cw allele was found in remaining 22 cases using the PCR-SSP technique. In the case of the serologically undetectable HLA-Cw blank antigens, the gene frequencies of Cw*12 and Cw*15 were 6.4% and 2.8%, respectively. CONCLUSIONS: These results indicated that serological typing is insufficient for accurate HLA-Cw typing. DNA typing using PCR-SSP technique appears a reliable and practical method for accurate HLA-Cw typing which can contribute to the evaluation of the biological role of the HLA-Cw in transplantation.

Analysis for misassignments of HLA-B homozygotes by serology and detection for HLA-B expression variants / 中国免疫学杂志

Objective:To find the discrepancies caused by HLA B gene variants in HLA B antigen homozygotes which were typed again by DNA method.Methods:75 blood samples assigned HLA B homozygotes by serology were typed and detected HLA B expression variants by PCR SSP.Results:The 12 samples had been assigned the second alleles by DNA method in 75 HLA B homozygotes by serology.One out of 12 samples was identified expression variant by PCR SSP.The null allele was caused by the insertion of an extra cytosine at the beginning of exon 4.Conclusion:HLA B gene typing by PCR SSP will be the use of supplement to serology.The expression variant detection by PCR SSP will improve accuracy for tissue typing and benefit clinical application.