RESUMO
OBJECTIVE: To study the chemical constituents in the whole plant of Huperzia leishangensis. METHODS: Compounds were isolated and purified by Prep-TLC, silica gel chromatography, Sephadex LH-20 and preparative high performance liquid chromatography, and their structures were identified on the basis of comprehensive analysis of their physical and chemical properties, NMR data and literatures. RESULTS: Ten compounds were isolated and elucidated as (15R)-12,16-epoxy-11,14-dihydroxy-8, 11, 13-abietatrien-7-one (I), 21-epi-serratenediol-3, 21-acetate (II), serratenediol-3-acetate (III), 21-epi-serratenediol-3-acetate (IV), 21α-hydroxy-serrat-14-en-3β-ol(V), 21β-hydroxy-serrat-14-en-3β-ol(VI), huperzine A (VII), sucrose (VIII), β-sitosterol (IX) and β-daucosterol(X). CONCLUSION: Compound I-X were for the first time isolated from H. leishangensis.