Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil / [Detecção molecular e aspectos clínicos da infecção por circovírus suíno 3 em suínos do Brasil]

Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.(AU)

O DNA do circovírus suíno 3 (PCV-3) foi detectado em amostras de soro de suínos aparentemente saudáveis, bem como em suínos com diferentes condições clínicas. A detecção molecular do PCV-3 foi observada em amostras de soro de suínos da região Sudeste do Brasil, com uma nested PCR desenhada especificamente para este estudo. A epidemiologia e os aspectos clínicos da infecção por PCV-3 foram avaliados. As amostras foram coletadas de 154 suínos de ambos os sexos, de diferentes fases de produção e com diferentes sinais clínicos. Os animais pertenciam a 31 granjas visitadas entre 2013 e 2018. Neste estudo, o PCV-3 foi detectado em 26,7% das amostras de animais saudáveis e de animais com variados sinais clínicos, de ambos os sexos e de idades variadas. Associação estatística (P=0,0285) foi observada apenas entre animais com sinais respiratórios e PCV-3; nenhum animal positivo para PCV-3 apresentava diarreia. Não foi observada associação estatística entre o PCV-3 e a idade ou o sexo dos suínos. Por se tratar de um vírus recém-descoberto, existem poucas informações sobre sua epidemiologia. Espera-se que os dados deste trabalho possam contribuir para futuros estudos sobre a epidemiologia do PCV-3.(AU)

Diagnóstico presuntivo de dengue en pacientes con fiebre y serología reactiva ingresados en un hospital pediátrico / Presumptive diagnosis of dengue in patients with fever and reactive serology admitted in a pediatric hospital

Se realizó un estudio descriptivo transversal de 1 819 pacientes con fiebre y serología reactiva, ingresados en el Hospital Infantil Dr Antonio María Béguez César de Santiago de Cuba por diagnóstico presuntivo de dengue, desde enero de 2015 hasta diciembre de 2016, de quienes se extrajeron muestras de suero en el Departamento de Microbiología de la mencionada institución, las cuales fueron enviadas al laboratorio del Instituto de Medicina Tropical Dr Pedro Kourí de La Habana para su procesamiento y posible confirmación. En la casuística se obtuvo una positividad de 54,3 por ciento, así como predominio de los niños mayores de 5 años con 1 451, de los procedentes del municipio de Santiago de Cuba con 1 651, de la fiebre en 847 y de la ocurrencia de la infección durante el trimestre octubre-noviembre en 864.

A descriptive cross-sectional study of 1 819 patients with fever and reactive serology, admitted in Dr Antonio María Béguez Caesar Pediatric Hospital in Santiago de Cuba was carried out through presumptive diagnosis of dengue from January, 2015 to December, 2016, from whom samples of serum were obtained in the Microbiology Department of the above-mentioned institution, which were sent to the laboratory of Dr Pedro Kourí Tropical Medicine Institute in Havana for their processing and possible confirmation. A 54,3 percent of positive cases was obtained in the case material, as well as the prevalence of children older than 5 years with 1 451, those coming from Santiago de Cuba municipality were 1 651, those with fever were 847 and those with occurrence of the infection during the trimester October-November were 864.

A novel luminol-based chemiluminescence method for the determination of amikacin sulfate in serum by using trivalent copper-periodate complex / 药物分析学报

A novel chemiluminescence (CL) reaction was based on the oxidizing reaction of luminol by the trivalent copper-periodate complex (K5[Cu(HIO6)2], DPC) in alkaline medium. The CL intensity could be enhanced in the presence of amikacin sulfate (AKS). A new CL method was developed for the determination of AKS by coupling with flow injection (FI) technology. Because of the distinctive oxidative effect of DPC, the luminol-based CL reaction could occur at a low concentration of 10à7 M. The relative CL intensity was proportional to the concentration of AKS in the range of 4.0 ? 10à9-4.0 ? 10à6 g/mL with the detection limit of 1.2 ? 10à9 g/mL. The relative standard deviation was 2.1% for 8.0 ? 10à9 g/mL AKS (n?9). The proposed method was successfully applied to the direct determination of AKS at the level of ng/mL in serum samples. The recovery varied from 97.0% to 106.3%. A possible mechanism of the CL reaction was discussed in detail by relating to the CL kinetic characteristics and electrochemical activities of the oxidant DPC.