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Objective To investigate the protective effect of Shen Mai injection in the treatment of lung injury induced by radiotherapy in patients with advanced breast cancer. Methods 80 cases with lung injury induced by radiotherapy in patients with advanced breast cancer from our hospital from 2015.1 to 2016.5 were selected and randomly divided into 2 groups, 40 cases in control group were given routine treatment, 40 cases in the experimental group were treated based on the control group treated with Shenmai injection treatment, and 2 groups were treated for 4 weeks. The pulmonary function, T lymphocyte subsets, clinical symptoms, quality of life scores and clinical efficacy were observed before and after treatment in two groups. The results were analyzed by statistical software SPSS 19.0. Results Compared with before treatment, levels of FVC, FEV1 and TLC in 2 groups after treatment elevated, scores of respiratory symptoms, activities, and daily life effect decreased, levels of serum CD3+, CD4+, CD4+/CD8+elevated, levels of CD8+ decreased, and compared with the control group, levels of FVC, FEV1, TLC, CD3+, CD4+, CD4+/CD8+ in the experimental group were higher, scores of respiratory symptoms, activities, and daily life effect were lower, clinical effect rate was higher, the differences were statistically significant (P<0.05). Conclusion Shen Mai injection can effectively improve lung function and immunity, improve respiratory symptoms and improve the quality of life in patients with advanced breast cancer radiotherapy for lung injury, and has better clinical efficacy.
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In this research,we chiefly explored a new fast test method--microtox technology to evaluate the comprehensive toxicity of Shen Mai injection.We investigated characteristic parameters of vibrio fischeri (CS234) under different conditions in the pursuit of the optimum test parameters of the reliable method;and then locked the best parameters for Shen Mai injection assay with the products from different manufacturers by microtox fast test.As a result,the optimum reaction condition included 0.9 mL recovery liquid in each freeze-dried vial and 50 μL bacteria suspension of each sample within 2 mL solution in aggregate with 10-mins detection after adding bacteria suspension which pH value ranging from 5 to 10 and luminous intensity from 0.8 to 1.2 million in 10 mins.The relative standard deviation values of replication experiment and precision test were all below 15％.Under this optimum detection condition,it was found that the EC50 values were 35.60％,92.34％ and 146.57％,differing among the samples from three representative drug manufacturers,respectively (P ＜ 0.01).In conclusion,the concentration-effect relationship of Shen Mai injection existed in the toxicity assessment of vibrio fischeri with various ECs0 values from the three manufacturers.These results suggested that biological detection standards of Shen Mai injection presented great growth potential.Microtox-based fast test in the evaluation of comprehensive toxicity of ShenMai injection showed favorable application prospects over controlling the quality of different sources of products.
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Objective: To investigate the effects of Shen-Mai injection(SM) on perfusion and oxygenation of intestinaltract during repeffusion in shocked rabbits. Methods:Twenty-one rabbits were divided into control group (Ⅰ,n = 6), shock-repeffusion group ( Ⅱ, n = 9) and SM group ( Ⅲ, n = 6). Intestinal intramucosal pH (pHi) of the sigrnoid colon and portalvein blood gas was observed before shock, at 1 hour after shock, 1 hour and 2 hours of reperfusion. Results: pHi and portal vein pH in group Ⅱ were significantly lower than those in group Ⅰ (P < 0.01), but portal vein increased obviously during reperfusion. There was a good linear positive correlation between pHi and portal vein pH. Portal vein in group Ⅱ was greater than that in group Ⅰ and had anegative correlation with pHi. pHi and portal vein pH of group Ⅲ increased signifi- cantly compared with those of goup Ⅱ ( P < 0.05), while of group Ⅲ returned to the level of group Ⅰ . MAP and CO of group m were higher than those of group Ⅱ after 1 hour or 2 hours reperfusion (P<0.05).CO of group m remained at a high level during reperfusion. But SVR of group Ⅲ was lower than that of group Ⅱ during reperfusion. Conclusion: SM im-proves perfusion and oxygenation of intestinal tract during reperfusion.