Short-and long-term safety of oral propranolol in the treatment of infantile hemangioma / 中华皮肤科杂志

Infantile hemangioma is the most common tumor in infancy,with an incidence of 4％-5％.According to the risk level,hemangioma is divided into 3 levels:high risk,medium risk and low risk.At present,the first-line treatment of high-risk hemangioma is oral propranolol (β-receptor blockers).It has been 10 years since infantile hemangioma was treated with propranolol for the first time,and propranolol has been the drug of first-choice in systematic treatment due to its high efficacy and good safety,but its long-term adverse effect needs further evaluation.

Adipose-Derived Stem Cells Improve Efficacy of Melanocyte Transplantation in Animal Skin

Vitiligo is a pigmentary disorder induced by a loss of melanocytes. In addition to replacement of pure melanocytes, cocultures of melanocytes with keratinocytes have been used to improve the repigmentation outcome in vitiligo treatment. We previously identified by in vitro studies, that adipose-derived stem cells (ADSCs) could be a potential substitute for keratinocytes in cocultures with melanocytes. In this study, the efficacy of pigmentation including durability of grafted melanocytes and short-term safety was examined in the nude mouse and Sprague-Dawley rat after grafting of primary cultured human melanocytes, with or without different ratios of primary cultured human ADSCs. Simultaneous grafting of melanocytes and ADSCs, which were separately cultured and mixed on grafting at the ratios of 1:1, 1:2, or 1:3, showed better efficacy than that of pure melanocytes. Grafting of melanocytes cocultured with ADSCs resulted in a similar outcome as the grafting of cell mixtures. Skin pigmentation by melanocytes : ADSCs at the ratios of 1:1 and 1:2 was better than at 1:3. No significant difference was observed between the 1-week and 2-week durations in coculturing. Time-course microscopic examination showed that the grafted melanocytes remained a little longer than 6-week post-grafting. No inflammatory cell infiltration was observed in the grafted skin and no melanocytes were detectable in other organs. Collectively, grafting of melanocytes and ADSCs was equally safe and more effective than grafting of melanocytes alone. Despite the absence of significant differences in efficacy between the group of 1:1 and that of 1:2 ratio, 1:2 ratio for 1-week coculturing may be better for clinical use from the cost-benefit viewpoint.