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Glycosylation is a part of the structure and function of immunoglobulin G (IgG). Sialic acid is located at the end of IgG N-glycan and regulates IgG anti-inflammatory and pro-inflammatory activities by changing the binding of IgG with fragment crystallizable gamma receptors (FcγRs) and complements. Low IgG sialylation is closely related to the occurrence, development and prognosis of autoimmune diseases and shows great potential in the field of diagnosis, monitoring and treatment, and may function as a new biomarker and therapeutic target for autoimmune diseases.
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Objective To investigate the level of fructosamine in different liver diseases.Methods From October 2016 to October 2017,153 patients with liver diseases hospitalized in Tongling Municipal Hospital were selected as study group,including 58 patients with chronic hepatitis B(CHB),35 patients with liver cirrhosis(LC),60 patients with primary hepatocellular carcinoma (HCC).Meanwhile,40 healthy subjects were selected as control group.The total protein (TP),albumin (ALB),cystatin C (CysC),sialic acid (SA) and fasting blood glucose (FBG) were statistically analyzed.Results The difference of fructosamine between the HCC group and the LC group,the CHB group and the control group was statistically significant[(299.1 ± 96.9)μmmol/L,(259.3 ± 41.5)μmmol/L,(248.7 ± 29.5) μ mmol/L,(234.9 ± 20.2) μmmoL/L] (F =11.321,P ＜ 0.05).There was no statistically significant difference in FBG between the study group and the control group (P ＞ 0.05).Correlation analysis showed that there was a positive correlation between fructosamine and FBG in the CHB group (r =0.655,P ＜ 0.05).The fructosamine of LC group was positively correlated with TP,CysC and FBG (r =0.406,0.418,0.351,all P ＜ 0.05).The fructosamine was correlated with TP,ALB,CysC and SA in HCC group(r =-0.513,-0.610,0.637,r =0.311,all P ＜ 0.05).There was no correlation between fructosamine and FBG in the HCC group (P ＞ 0.05).Logistic stepwise regression analysis showed that in the HCC group,fructosamine (OR =1.029,95 ％ CI:1.007 ～ 1.052),CysC (OR =5 454.909,95％ CI:78.117 ～ 380 916.873) and SA(OR =1.155,95％ CI:1.047 ～ 1.275) were independent risk factors of HCC.Conclusion It has positive correlation between fructosamine and FBG in CHB and LC patients,fructosamine may not be related to glycometabolism in HCC patients,but the data of fructosamine reflect the degree of glycosylation of HCC tumor cells.
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Objective@#To investigate the level of fructosamine in different liver diseases.@*Methods@#From October 2016 to October 2017, 153 patients with liver diseases hospitalized in Tongling Municipal Hospital were selected as study group, including 58 patients with chronic hepatitis B(CHB), 35 patients with liver cirrhosis(LC), 60 patients with primary hepatocellular carcinoma(HCC). Meanwhile, 40 healthy subjects were selected as control group.The total protein(TP), albumin (ALB), cystatin C(CysC), sialic acid(SA) and fasting blood glucose(FBG) were statistically analyzed.@*Results@#The difference of fructosamine between the HCC group and the LC group, the CHB group and the control group was statistically significant[(299.1±96.9)μmmol/L, (259.3±41.5)μmmol/L, (248.7±29.5)μmmol/L, (234.9±20.2)μmmol/L](F=11.321, P<0.05). There was no statistically significant difference in FBG between the study group and the control group(P>0.05). Correlation analysis showed that there was a positive correlation between fructosamine and FBG in the CHB group(r=0.655, P<0.05). The fructosamine of LC group was positively correlated with TP, CysC and FBG (r=0.406, 0.418, 0.351, all P<0.05). The fructosamine was correlated with TP, ALB, CysC and SA in HCC group(r=-0.513, -0.610, 0.637, r=0.311, all P<0.05). There was no correlation between fructosamine and FBG in the HCC group (P>0.05). Logistic stepwise regression analysis showed that in the HCC group, fructosamine(OR=1.029, 95% CI: 1.007~1.052), CysC (OR=5 454.909, 95% CI: 78.117~380 916.873) and SA(OR=1.155, 95% CI: 1.047~1.275) were independent risk factors of HCC.@*Conclusion@#It has positive correlation between fructosamine and FBG in CHB and LC patients, fructosamine may not be related to glycometabolism in HCC patients, but the data of fructosamine reflect the degree of glycosylation of HCC tumor cells.
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Objective To investigate the clinical significance of serum sialic acid (SA) in the diagnosis and treatment of IgA type multiple myeloma (MM). Methods The level of serum SA in 50 healthy subjects and 70 patients with MM were determined. Results In MM group, there were 25 cases of IgA type, 51 cases of other than IgA type (40 cases of IgG type, 7 cases of light chain type MM, 4 cases of non-secreting type MM). In healthy subjects, patients with IgA, IgG, light chain, non-secreting types and other than IgA type, the serum SA levels were (570.33 ± 67.72) mg/ L, (1289.24 ± 325.42) mg/ L, (585.88 ± 159.12) mg/L, (600.77 ± 126.90) mg/L, (590.50 ± 100.86) mg/L, and (588.39 ± 150.90) mg/L. The serum SA level of IgA type patients was higher than those of healthy subjects and other types (all P 0.05). The serum SA level of IgA type patients had positive correlations with serum IgA, globulin, bone marrow smear myeloma cell population and ESR (r values were 0.699, 0.753, 0.504, and 0.732, all P0.05). The level of serum SA in IgA type patients decreased with the improvement of the condition, and increased with the severity of the disease. Conclusions In MM patients, serum SA level in IgA type is high. The serum SA is an index for reflecting the tumor load capacity, and can evaluate the therapeutic efficiency of IgA type MM patients.
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Objective To discuss the diagnostic value of serum sialic acid for glioma.Methods A retrospective study was conducted.The levels of sialic acid in serum samples of 95 glioma patients, 175 patients with brain benign tumor and 400 normal persons from October 2014 to March 2015 were detected by automatic biochemistry analyzer using enzymic method.The SNK-q test and analysis of variance were used to compare the difference of the groups.By making receiver operating characteristic ( ROC) curve, the cut-off value, sensitivity, specificity, positive predictive value and negative predictive value were calculated to assess the diagnostic value of serum salivary acid.Then the cut-off value was validated by using the serums of 30 glioma patients and 30 normal persons who were out-patients and healthy controls.Results The levels of serum sialic acid in patients with gliomas, patients with brain benign tumor and healthy individuals were (0.66 ±0.14 ) g/L, (0.61 ±0.09 )g/L, (0.54 ±0.07 )g/L.The serum salivary acid of glioma patients were higher than brain benign tumor patients (q=6.74,P0.05) among the glioma patients of different grades (8 of gradeⅠ,32 of gradeⅡ,24 of grade Ⅲ,31 of grade Ⅳ).There was no significant difference between the low grade patients (gradeⅠandⅡ) and the high grade patients (gradeⅢandⅣ) (t=0.55, P>0.05), but the level of serum sialic acid of high grade group had an increasing trend than the low grade group.The area under the ROC curves was 0.79.The cut-off value of serum salivary acid for diagnosing glioma was 0.61 g/L.The sensitivity was 67.74%, and the specificity was 80.60%.The positive predictive value was 44.68%, and the negative predictive value was 90.76%.Then the cut-off value was used as a diagnostic criteria, and the detected results of 30 glioma patients and 30 normal persons showed that the sensitivity was 63.30% and the specificity was 83.30%.Conclusions The serum sialic acid has good specificity and negative predictive value for diagnosing glioma.It may be a valuable diagnostic marker.
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Sialic acids (Sias) are nine-carbon keto sugars primarily present on the terminal residue of cell surface glycans. Sialic acid binding immunoglobulins (Ig)-like lectins (siglecs) are generally expressed on various immune cells. They selectively recognize different linkage-specific sialic acids and undertake a variety of cellular functions. Many pathogens either synthesize or acquire sialic acids from the host. Sialylated pathogens generally use siglecs to manipulate the host immune response. The present review mainly deals with the newly developed information regarding mechanism of acquisition of sialic acids by pathogens and their biological relevance especially in the establishment of successful infection by impairing host innate immunity. The pathogens which are unable to synthesize sialic acids might adsorb these from the host as a way to engage the inhibitory siglecs. They promote association with the immune cells through sialic acids-siglec dependent manner. Such an association plays an important role to subvert host’s immunity. Detailed investigation of these pathways has been discussed in this review. Particular attention has been focused on Pseudomonas aeruginosa (PA) and Leishmania donovani.
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Objective To investigate the expression and distribution of sialyl Lewis X in colorectal carcinoma and its relationship with carcinogenesis, progression and metastatic proclivity.Methods Microwave LSAB immunohistochemical technique was used to detect the expression of sialyl Lewis X in colorectal carcinoma and in normal mucosa. Immunoelectromicroscopic localization of sialyl Lewis X labelled by colloidal gold was also observed.Results The positive rate of sialyl Lewis X expression in primary colorectal cancer was 92 2%(83/90), and 16 7%(5/30) in normal mucosa. The positive rate was 100% in patients with lymphatic nodes metastasis, compared with that of negative nodes of 82 1% ( P
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ObjectiveTo explore the clinical significance and characteristics about terminal sialyl residues linked to N-glycans in the tissue and bile glycoproteins from bile duct carcinoma. MethodsThe mRNA expression of sialyltransferases, including ST3Gal-Ⅲ and ST6Gal-Ⅰ, was detected in the tissue extracts of 35 cases with bile duct carcinoma(BDC) and 35 cases of benign biliary disease (BBD) by semiquantitative reverse transcription-polymerase chain reaction(RT-PCR). With the lectin of WGA(Triticum valgaris agglutinin), which can recognise the terminal sialyl residues in N-glycans, all the specimens of BDC and BBD were subject to lectin histochemical staining, followed by computer-aided digital analysis. Lectin dot-blotting with horseradish peroxidase-labeled WGA was performed for the bile samples from each of the 35 cases of BDC and BBD. ResultsThe mRNA levels of ST3Gal-Ⅲ and ST6Gal-Ⅰ elevated in BDC(P
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Objective To analyze the active principle of Melagenine and its effect on melanocytes of guinea epidermis.Methods The content of endothelins and sialic acid of Melagenine were determined by radioimmunoassay and spectrophotometer.The skin of the back of guinea pigs was treated with Melagenine and irradiated with infrared-ray.Then the biopsy specimens were taken from the treated and untreated back skin,sections and supe-rthin sections were prepared for special staining and TEM examination,respectively,the number melanocytes and melanin content index(MCI)were measured.Results The contents of en-dothelins and sialic acid in melagenine were210.5?30.1pg/mL and147.9?12.1?g/mL,respectively;the number of melanocytes,the keratinocytes with melanin granules and the melanin content index were all in-creased significantly in the treated skin.Conclusions There are endothelins,carbohydrates and glycolipids in melagenine,endothelins can promote the proliferation of melanocytes,while carbohydrates and glycolipids can enhance intercellular recognition and adhesion.The result of the study shows that melagenine promotes the proliferation of melanocytes and synthesis of melanin in the skinof guinea pigs.
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cholesterol/phospholipid ratio.