Chemical Constituents from the Aerial Part of Sibiraea angustata / 中国药学杂志

OBJECTIVE: To study the chemical constituents of the aqueous extract from the aerial part of Sibiraea angustata. METHODS: The constituents were isolated by various chromatographic techniques(HP - 20 macroporous absorption resin, Sephadex LH - 20 gel, Reverse-phase silical gel and PHPLC) and their structures were determined on the basis of physicochemical properties and their spectroscopic data, as well as the literatures. RESULTS: Twelve compounds were separated and identified as veratric acid (1), (+) -cycloolivil(2), 3, 7-dimethyl-3(E) -6-octadien-5-one-1-O-β-D-glucoside(3), 3, 7-dimethyl-3(Z) -6-octadien-5-one-1-O- β-D-glucoside(4), 1-O-β-D-glucopyranosyl(1→2) -β-D-glucopyranosyl-3, 7 -dimethyl-2(E) -6-heptdiene(5), (7R, 8 S) -dihydrodehydrodiconiferyl alcohol-9'-O-β-D-glucopyranoside(6), (+) -1-hydroxypinoresinol-1-β-D-glucoside(7), skimmin(8), kaempferol 3-O-α- L-arabinopyranosyl-(1→6) -β-D-galactopyranoside (9), isorhamnetin-3-O-β-D-galactopyranosyl (1→6) -β-D-glucopyranoside (10), isorhamnetin 3-O-α-arabinopyranosyl-(1→6) -β-galactopyranoside(11), and quercetin 3-O-[2‴ -O-(E)-caffeoyl]-α-L-arabinopyranosyl-(1→6) -β-D-galactopyranoside(12). CONCLUSION: All compounds are obtained from the genus of Sibiraea for the first time.

Optimization of Extraction and Enrichment Process and Anti-hypoxia Activity of Total Triterpenes from Sibiraea Angustata / 中国中医药信息杂志

Objective To optimize the extraction and enrichment process of total triterpenes from Sibiraea Angustata; To study its anti-hypoxia activity. Methods The extraction solvent of total triterpenoids of Sibiraea Angustata was screened by atmospheric pressure anti-hypoxia test. The content of total triterpenes and extraction rate were set as evaluation indexes. The effects of extraction time, extraction times and ratio of material to liquid on extraction were investigated by orthogonal test. The extraction process was optimized. Resin type, concentration of sample, eluent concentration and eluent volume were investigated to optimize the enrichment process of total triterpenes of Sibiraea Angustata. Finally, the anti-hypoxia activity was studied by atmospheric pressure test and acute decompression test. Results The optimum extraction process was as follows: 70% ethanol extraction, the ratio of material to liquid was 1:20, extrat 2 times with 1.5 h for each time, and the content of total triterpenes was 32.25%. The optimum enrichment process was: AB-8-type macroporous adsorption resin, the loading concentration of 2 mg/mL, 80% ethanol 5 BV elution, and total triterpenes content was up to 61.09%; Sibiraea Angustata total triterpenes anti-hypoxia activity test was strong, and had a certain dose dependency. Conclusion The extraction and enrichment process of total triterpenes from Sibiraea Angustata is stable, reproducible and has significant anti-hypoxia activity.

Progression of Medicinal Effects of Sibiraea angustata / 世界科学技术-中医药现代化

Sibiraea angustata belongs to the Rosaceae family and it is widely disturbed in Qinghai,Sichuan,Gansu,Yunnan and Tibet.It is one of the commonly used Tibetan medicines.Sibiraea angustata is rich in triterpenes,organic acids,organic esters,alkanes,and etc.And studies showed that Sibiraea angustata has various medicinal effects,such as regulating the metabolism of glucose and lipid,reducing body weight,anti-oxidation,modulating the immune system,liver-protection,anti-tumor,anti-aging,antibacterial activities,and promoting digestion.