RESUMO
Objective To discuss the therapeutic significance of granulocyte colony stimulating factor (G-CSF) in the treatment of invasive Candida infections in congenital immunodeficiency,especially in cases with low level of interleukin 17(IL-17). Methods Therapeutic processes of 2 children suffering from invasive Candida infections secondary to congenital immunodeficiency were retrospectively studied,further-more,the related literatures were also reviewed. Results Whole-exome sequencing revealed CARD9 muta-tion in the first patient, who suffered from Candida albicans meningoencephalitis, while the other patient revealed STAT1 mutation suffering from recurrent lung abscess caused by Candida albicans,and both of them showed low level of blood IL-17. Routine antifungal drugs were insufficiency,even treatment period had pro-longed for several months. But after the G-CSF adjuvant therapy,their symptoms,signs and images recov-ered. With 5 to 10 months follow-up,no recurrent infections were found. Conclusion Antifungal drugs com-bined with G-CSF can effectively improve the treatment effect in invasive fungal infections secondary to immunodeficiency. Further more,genetic screening especially whole-exome sequencing can be suggested to find unusual immunodeficiency,which helps more individualized treatment.
RESUMO
Aim To investigate the effects of losartan on activation of signal transducers and activators of transcription 1(STAT1) protein and the expression of STAT1 mRNA in glumeruli of diabetic rats.Methods Wistar male rats were randomly divided into three groups: control group, diabetes group and treatment group.Diabetes was induced by intraperitoneal injection of STZ (65 mg?kg~-1 ). Losartan (40 mg?kg~-1 ) was administered daily by gavage from the next day of the induction to diabetes for 2 weeks. The protein expression of p-STAT1 in glomeruli was observed by immunohistochemistry and western blot. STAT1 mRNA was measured by reverse transcription and polymerase chain reaction (RT-PCR).Results Compared with the control group rats, the expression of p-STAT1 was significantly increased in glomeruli in the diabetic rats (P