RESUMO
Objective To investigate the antibacterial properties and curative effects of a nanosilver-epidermal growth factor (NanoAg-EGF) sustained-release carrier.Methods First,in accordance with former method,sustained-release carrier of NanoAgEGF was prepared,with the design of the NanoAg-EGF group,NanoAg-alone group,EGF-alone group,control groups of normal saline-alone,and benzylpenicillin sodium.Contrasting detection on antibacterial properties of five kinds of common microbes (staphylococcus aureus,escherichia coli,pseudomonas aeruginosa,candida albicans,and streptococcus pneumonia) in each group was used to confirm their antibacterial properties.Then,wound models of diabetic rats were randomly divided into five groups,with the wound treatments by sustained-release cartier of NanoAg-EGF,combined application of NanoAg + EGF,EGF-alone,NanoAg-alone,and normal saline-alone control group,respectively.The observation on the healing situation of each group in different times was used to ensure the curative effect.Results In the antibacterial experiments,it was found that the NanoAg-EGF and NanoAg had strong antibacterial properties.Their antibacterial properties had no significant difference (P ＞ 0.05).The control group,penicillin sodium only had relatively weak antibacterial properties on staphylococcus aureus and streptococcus pneumoniae,with obviously weaker function compared to the NanoAg-EGF and NanoAg groups (P ＜ 0.05).The control groups,EGF-alone and normal saline-alone had no inhibitor function on each kind of pathogenic microorganisms.In the wound healing experiments on diabetic rats,it was found that NanoAgEGF shortened significantly the wound healing time of diabetic rats (P ＜ 0.05).On the 3rd day,the healing rate of each group had no difference (P ＞ 0.05).On the 7th day and 13th day,the healing rates of NanoAg-EGF [(61.71 ± 8.78)％ and (100 ± 2.60) ％] were significantly higher than the other four groups (P ＜ 0.05).Conclusions NanoAg-EGF not only has good antibacterial properties but also can promote rapidly the wound healing of diabetes.
RESUMO
Introdução: Pacientes vítimas de queimaduras apresentam reação inflamatória sistêmicaque modifica o metabolismo, podendo cursar com infecção da área queimada. A enxertiade pele é uma ferramenta no tratamento dos queimados, no entanto, a infecção diminuiou impossibilita a integração dos enxertos. O uso de curativos com prata tem eficácia naprevenção e no tratamento de áreas contaminadas. A prata nanocristalina apresenta liberaçãohomogênea e manutenção do ambiente próprio para reepitelização. O objetivo dessetrabalho é descrever a eficiência do curativo com prata nanocristalina sobre enxerto emmalha em queimaduras com colonização por cepas multirresistentes. Método: Foram avaliadosquatro pacientes com queimaduras colonizadas, realizados enxerto e cobertura comcurativo de prata nanocristalina. Resultados: Todos os pacientes apresentaram melhora naintegração do enxerto.
Introduction: Burn patients have systemic inflammatory reaction that modifies the metabolismand may result with infection of the burned area. Skin grafting is a tool in the treatmentof burns, however, the infection decreases or prevents the integration of the grafts. The use ofdressings with silver is effective in the prevention and treatment of contaminated areas. Thenanocrystalline silver has released homogeneous and maintenance of proper environmentfor reepithelialization. The aim of this article is to describe the efficiency of nanocrystallinesilver dressings on graft in place burning with colonization by multi-drug-resistantstrains. Methods: We evaluated four patients with burns colonized, performed graft andcovered with nanocrystalline silver dressing. Results: All patients showed improvementof graft integration.