Identification of adult worm of human small liver fluke in Nghe An and Dong Nai province

Since 2002, adult worms of human small liver fluke in some provinces were identified by morphology and confirmed by molecular method as Clonorchis sinensis in Thanh Hoa, Ninh Binh, Nam Dinh and Bac Giang province; Opisthorchis viverrini in Phu Yen and Binh Dinh province. This study, using polymerase chain reaction (PCR), a portion of the cytochrome oxidase 1 (410 nucleotide and 136 amino acid) of mitochondrial genome of adult worms of small liver fluke from human in Nghe An (CsNA) and Dong Nai (CsDN) province were amplified. The nucleotide and amino acid of these sequences was comparatively analyzed with the known Clonorchis sinensis (Nam Dinh strain). The analysis revealed that the Nghe An Clonorchis sp and Dong Nai Clonorchis sp (in human) were homology (99.5 - 99.8% nucleotide) and similar (100% amino acid) to the Nam Dinh Clonorchis sinensis (identification was done). Thus, the Nghe An and Dong Nai Clonorchis sp were identified as Clonorchis sinensis in Opisthorchiidae family, Clonorchis genus.

Current infection with small liver fluke at 2 communes Xuan Tien and Xuan Chau of Xuan Truong district, Nam Dinh province

Background: Liver flukes is classified into Foodbone Trematodes, which can cause serious harmful effect to human health such as cirrhosis. Objective: To identify the infection rate of small liver fluke at 2 communes Xuan Tien and Xuan Chau of Xuan Truong district, Nam Dinh province. Subject and Method: The descriptive epidemiological study with a random sampling was conducted on 1411 people aged 18 or higher to find small liver fluke eggs in their feces by Kato's method. Result and Conclusion: Findings show that the infection rate of small liver fluke in people was 19.1 %, and that in Xuan Tien was higher than that in Xuan Chau (29.7% vs. 6.2%). The infection rate was highest in the 40-49 year old group (28.4%), and lowest in the 18-29 year old group (7.6%). Males had a higher infection rate than females (31.0% vs.10.5%). By occupation, the home craft had the highest infection rate (39.1 %), followed by farmers (20.1 %) and some others (6%). Those who completed secondary school or lower education had a infection rate higher than that of those who completed high school (24.1 % vs. 8.8%).