RESUMO
Objective To study the chemical components from the roots of Smilacina henryi. Methods Silica gel, Sephadex LH-20, and preparation of thin layer chromatography techniques were used for isolation, and 1D, 2D-NMR, IR, and HR-ESI-MS data were used for structure identification. Results Seven steroidal components were obtained and its chemical structures were elucidated as (25S)-5α-spirostan-9(11)-en-3β,17α-diol 3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside (1), (25S)-5α-spirostan-9(11)-en- 3β,17α-diol (2), (25S)-5α-spirostan-9(11)-en-3β,17α-diol 3-O-β-D-glucopyranoside (3), diosgenin (4), aspidistrin (5), henryioside A (6), and henryioside B (7). Conclusion Compound 1 is a new steroidal saponin named henryioside D, and compounds 2-5 are isolated from the plant for the first time.