RESUMO
Objective Hypoxic-ischemic brain damage (HIBD) is a life-threatening neonatal disease during perinatal stage. Since excitatory amino acids (EAAs) and their receptors are involved in the pathogenesis. Sodium hydroxybutyrate ( r-OH ), an intermediate metabolite of GABA, may have beneficial effects on HIBD. Methods One-hundred seven-day (7d) SD rat pups were randomly assigned to one of three groups:(Ⅰ) control group (n= 20);(Ⅱ) sham operation group (n=20)and (Ⅲ) r-OH group which was further divided into 3 subgroups according to the dose of r-OH 50 mg?kg-1 (r-OH1) (n=20),100 mg?kg-1 (r-OH2) (n=20),200mg?kg-1 (r-OH3) ( n=20).Animals in control group and r-OH group were subjected to left carotid artery ligation followed by 2 h of hypoxia (O2 :N2=8%:92%).Normal saline ( NS) was administered ip immediately after sham operation or hypoxia,then 3 times a day for 7 days in group Ⅰ and Ⅱ.In r-OH group r-OH was administered ip instead of NS. Brain damage was evaluated by survival rate, pathology, the ratio of weight of left to right hemisphere on the 28 th day after ischemia-hypoxia and the capacity of learning and memory using Y-Maze test. Results (1) The survival rate on the 28 th day after hypoxia or sham operation was significant lower in control group (60%) than that in the other groups (85%-95% ) (P
RESUMO
The effects of intravenous anesthetics on phagocytosis of human neutrophils were observed in vitro. 1?10~6/ml of neutrophils, isolated from heparinized venous blood of healthy human,was mixed at equivalent volume with normal saline (as control), four different concentrations (clinical doses) of droperidoI, midazolam, fentanyl,etomidate or sodium hydroxybutyrate respectively. All mixtures were incubated in each of four 24-pore culture plates. The equivalent volumes of staphylococci 6～9?10~9/ml were added to these mixtures, 10 mins, 30 mins, 2hs or 24hs after incubation individually. The successive incubations lasted 45 mins,and the neutrophil phagoeytosis rate (NPR) was calculated. The results showed that NPR was correlated negatively with the concentration of each anesthetic mentioned above. but was related to its acting-duration. It is demonstrated that these intravenous anesthetics may reduce the phagoeytosis of neutrophils in a dose-related way,therefore we suggest that antibiotics or antineoplastics should be used in combination with these anesthetics during operation.
RESUMO
The effects of exogenous Na-DL-3-hydroxybutyate (3-HOBNa)and insulin(I) on skeletal muscle protein metabolism in rats inflicctcd with 37% TBSA full thickness scalding were studied It was found that the scrum acctoacctatc (AcAc) of the burned animals showed no significant difference from the control on the 4th day postburn,but the free fatty acid (FFA) decreased markedly and the urinary 3 - methylhistididne(3-MH) excretion and the protein de -gradation rate of the soleus muscle of the injured hind limb increased na kedly.Infusion of 3-HOBNa for 3 consecutive days following burns could lower the urinary 3-MH excretion and the protein,degradation rate of the soleus muscle with no significant influence on the skeletal muscle protein anabolism.Moreover,investigations revealed that there was no syncrgistic effect of insulin-kctone body combinalioan on the anti-catabolic capacity in burned rats.