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Objective To evaluate the clinical values of serum soluble CD44 (solCD44) in diagnostic classification of acute myeloid leukemia (AML).Methods 80 patients with AML who admitted in Department of Hematology in our hospital from January 2014 to January 2016 were enrolled.Among these 80 cases,there were 31 cases newly diagnosed,24 cases refractory and 25 cases remission.40 healthy volunteers were set as the control group.The levels of solCD44s,solCD44v5 and solCD44v6 were measured and compared of each group.Results The levels of serum solCD44s,solCD44v5 and solCD44v6 in newly diagnosed and refractory group of AML patients were significantly higher than those in the control group and remission group (P0.05);There were significant differences of serum levels of solCD44s,solCD44v5,solCD44v6 in different FAB subgroups (M1+M2),M3,M4 and M5,meanwhile,the serum levels of solCD44s,solCD44v5,solCD44v6 in M3 type were significantly higher than those in non-M3 type (P<0.05).Conclusion The level of solCD44 especially solCD44std is closely related to the changes of disease activity in patients with AML.Serum solCD44 levels can be used as serological reference indicators of diagnostic classification,prognosis and recurrence prediction for AML.
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BackgroundResearches demonstrated that the levels of soluble CD44 (sCD44)molecule in aqueous is significantly higher in primary open-angle glaucomous(POAG) eye than normal eye,but how the sCD44 would affect the expression of apoptosis protein in trabecular meshwork cells is below understanding.Objective The present study was to investigate the effect of sCD44 on the expression of regulatory proteins bcl-2 associated death factor bad in trabecular meshwork cells in the patients with POAG.MethodsHuman scleral tissue with trabecular meshwork were obtained from POAG patients during the surgery.The trabecular meshwork cells were primarily cultured by explant culture method and identified by immunochemistry.The third generation of cells were incubated with free-serum DMEM/F12 medium added differnt dosages of sCD44 (0,1,5,10,25,50 mg/L) for 48 hours.The expression of bad protein in cultured cells was detected using cell counting kit-8 (CCK-8) as the absorbance values at 490 nm(A,90 value),and the bad protein level in cultured cells was assayed by ELISA.ResultsThe cultured cells showed the positive response for laminin ( LM ),neuron specific enolase ( NSE ),fibronectin ( FN ) monoclonal antibodies.The CCK-8 assay showed that the A490 values of the trabecular meshwork cells in 0,1,5,10,25,50 μg/L of sCD44 groups were 0.2460±0.0019,0.1874±0.0015,0.1570±0.0016,0.1302±0.0019,0.1084±0.0018,0.0940±0.0020 respectively with a statistically significant difference among the 6 groups( F =14.922,P =0.000 ),and the A490 values in various dosages of sCD44 groups were significantly lower than the 0 μg/L sCD44 group (P=0.013,0.008,0.011,0.005,0.004).The ELISA assay showed that bad protein levels in 0,1,5,10,25,50 μg/L of sCD44 groups were ( 114.8461 ± 2.9560 ),( 137.8270 ± 2.4259 ),( 161.4194 ± 3.7381 ),( 170.9453 ± 3.2006 ),( 221.2252 ±4.3738 ),( 324.6167±4.4220) ng/L,showing a total difference among them ( F =16.610,P =0.000 ),and the bad protein levels in various dosages of sCD44 groups were significantly lower than the 0 μg/L sCD44 group( P =0.017,0.013,0.008,0.007,0.006).ConclusionssCD44 can contribute to the apoptosis of the trabecular meshwork cells in patients with POAG in certain dose range by regulating the apoptosis regulatory proteins bcl-2 associated death factor bad.