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Objective: To study the chemical constituents of Stemona tuberosa. Methods: The chemical constituents were isolated and purified by chromatography with RP-C18, silica gel, Sephadex LH-20 and semi-preparative HPLC, and their structures were identified by analysis of spectroscopic data and physicochemical properties as well as relevant references. Results: Ten compounds were isolated from the roots of S. tuberosa, and they were elucidated as 6-methoxy-3-methyl-2H-pyran-2-one (1), stilbostemin A (2), stilbostemin K (3), stilbostemin P (4), stilbostemin Q (5), stilbostemin R (6), stilbostemin T (7), stilbostemin W (8), stilbostemin X (9) and stilbostemin Y (10). Conclusion: Compound 1 is the new pyrone derivative named as stemonapyrone A. Compounds 2 and 3 are isolated from the plant of S. tuberosa for the first time.
RESUMO
Objective To analyze the relationship and genetic diversity of Stemona tuberosa in different populations. Methods ISSR molecular technique was be used to decipher the genetic diversity of S. tuberosa in South of the Yangtze River, The genetic relationships among different populations were analyzed based on software POPGEN32 and the DNA molecular dendrogram was structured according to the software NTSYSpc-2.10E. Results Seventy-four obvious bands of different S. tuberosa populations were amplified with seven suitable ISSR primers, 74 of them were polymorphic sites, and the percentage of polymorphism bands reached to 100%; Effective number of alleles (Ne) is 1.524 4, the average Nei's genetic diversity (He) index is 0.314 6, the average Shannon's information index is 0.478 6, the genetic distance among populations is 0-0.950 2, and the average distance is 0.416 3. Conclusion The study revealed that a relative high genetic diversity occurred in different populations of S. tuberosa, the genetic variation is related to geographical distance in different populations of S. tuberosa. The study will throw light on how to introduce and cultivate traditional Chinese medicinal plant, how to screen peculiar pyrrolidine alkaloids from S. tuberosa, and how to build to scientific standards and theoretical basis for traditional geo-authentic crude drug in China.
RESUMO
A high throughput UHPLC-QTOF/MS method was established to analyze and identify the constituents in rat plasma after oral administration of honey-fried Stemona tuberosa Lour. extract. Automated data analysis software MetaboLynx was developed in analysis and identification of the bioactive components and their metabolites in rat plasma following oral administration of honey-fried S. tuberosa Lour. extract. As a result, 40 compounds including 12 prototype components and 28 metabolites were characterized. The constituents absorbed into blood and the possible metabolites which were demonstrated to originate from the active fraction of honey-fried S. tuberosa Lour. were responsible for the observed efficacy. Its serum pharmacochemistry should be subjected to complete investigation to illuminate the pharmacology and active mechanism of honey-fried S. tuberosa Lour.