RESUMO
OBJECTIVE：To investigate the correlation of storage life and effective composition content with color value of Carthamus tinctorius ，and to provide reference for the quality evaluation of C. tinctorius with different years of storage. METHODS：Using 24 batches of C. tinctorius from same place of production with different years of storage （0，1，2 years，8 batches each type ）as samples ，the contents of hydroxysafflor yellow A （HSYA）and kaempferol were determined by HPLC. Color value [lightness value （L*），red-green value （a*），yellow-blue value （b*）] were determined by spectrophotometer. SPSS 19.0 statistical software was used to analyze the correlation of storage life and effective composition content with color value. RESULTS：Kaempferol content was still high after 1 year or 2 years of storage （0.161％，0.061％，respectively）. However ，the content of HSYA decreased with the prolongation of the storage life （the average content of HSYA were 2.46％，1.58％，and 1.51％ after storage 0，1 and 2 years，respectively），and the color of the drug became darker （a* value decreased ）. Results of correlation analysis showed that the content of HSYA was positively associated with color value L*，a*（r＝0.430，0.781，P＜0.05 or P＜0.01）；the content of HSAY was negatively associated with storage life （r＝－0.777，P＜0.01）. There was no correlation between the remaining variables （P＞0.05）. CONCLUSIONS ：The longer the storage life ，the darker the color and the lower the content of HSYA ，so it is not suitable for over year and multiyear preservation.
RESUMO
Functional spent hen meat nuggets (SMN) both unwrapped and wrapped in bioactive biodegradable films were evaluated for their storage stability at refrigeration temperature (4 ±1ºC) under different packaging conditions. Standardized zein films formulated with 5% zein proteins and 7% glycerol and incorporated with 5% coliphages having concentration of 1010 pfu/ml were utilized in this study. Different treatments such as T1 (product packaged in aerobic conditions), T2 (product packaged in modified atmospheric packaging [MAP] conditions), T3 (product packaged in vacuum), T4 (product wrapped in developed films and packaged in aerobic conditions), T5 (product wrapped in developed films and packaged in MAP conditions), T6 (product wrapped in developed films and packaged in vacuum conditions) were evaluated at weekly interval for 5 weeks for their physico-chemical, sensory and microbiological parameters. Results depicted that T5 (product wrapped in developed films and packaged in MAP conditions) had comparatively lower value for TBARS, Free fatty acids, peroxide value and standard plate count than other treatments. Psychrophiles, yeast and mold, E.coli, coliforms were completely absent in the T5 throughout the storage period. All vacuum and MAP packaged products had better sensory attributes throughout storage than aerobic packaged products. It was thus concluded that developed SMN can be successfully stored for 35 days in bioactive biodegradable wrap under MAP conditions at 4±1oC