Evaluation of Mexican sunflower (Helianthus annus l) leaf meal as a feed ingredient in shaver brown pullets

Abstract The use of alternative nutritional sources is an important topic for animal production such as poultry. This study examined the effect of replacing soybean meal with sunflower leaf meal in the diet of laying shaver brown pullets. A total of 30 laying birds were assigned to three treatments of 0% (as control group), 10% and 15% MSFLM inclusion. Four eggs per replicate were randomly taken for three consecutive days at two weeks interval for five times, and 18 weeks individuals were selected after 40 days of experimental time. External parameters of the eggs (egg weight, egg length, egg breadth and egg shape index, shell thickness), and body parameters (Final body weight, weight gain), feed intake and hen day production were measured. For egg length, egg breadth and shell thickness showed significant difference (p 0.05) in from the control birds. Final body weight, weight gain, feed intake and hen day production showed comparable results with values from eggs of birds on control diet. The study revealed the ability of birds to easily utilize the nutrients in the protein sources. MSFLM utilization up to 15% in pullets diet revealed no detrimental effect on the performance of the laying birds and the external qualities of eggs produced.

Resumo O uso de fontes nutricionais alternativas é um tópico importante para a produção animal, como a avicultura. Este estudo examinou o efeito da substituição do farelo de soja por farelo de folha de girassol na dieta de frangas marrons barbeadoras poedeiras. Um total de 30 aves poedeiras foram designadas a três tratamentos de 0% (como grupo controle), 10% e 15% de inclusão MSFLM. Quatro ovos por repetição foram retirados aleatoriamente por três dias consecutivos em intervalos de duas semanas por cinco vezes, e indivíduos de 18 semanas foram selecionados após 40 dias do tempo experimental. Parâmetros externos dos ovos (peso do ovo, comprimento do ovo, largura do ovo e índice de forma do ovo, espessura da casca) e parâmetros corporais (peso corporal final, ganho de peso), consumo de ração e produção diária da galinha foram medidos. Para comprimento do ovo, largura do ovo e espessura da casca apresentaram diferença significativa (p 0,05) nas aves controle. Peso corporal final, ganho de peso, consumo de ração e produção de dia de galinha mostraram resultados comparáveis com valores de ovos de aves em dieta controle. O estudo revelou a capacidade das aves de utilizar facilmente os nutrientes das fontes de proteína. A utilização de MSFLM até 15% na dieta das frangas não revelou nenhum efeito prejudicial no desempenho das aves poedeiras e nas qualidades externas dos ovos produzidos.

Evaluation of Mexican sunflower (Helianthus annus l) leaf meal as a feed ingredient in shaver brown pullets / Avaliação do girassol mexicano (Helianthus annus l) farinha de folha como ingrediente de ração em frangas marrons shaver

The use of alternative nutritional sources is an important topic for animal production such as poultry. This study examined the effect of replacing soybean meal with sunflower leaf meal in the diet of laying shaver brown pullets. A total of 30 laying birds were assigned to three treatments of 0% (as control group), 10% and 15% MSFLM inclusion. Four eggs per replicate were randomly taken for three consecutive days at two weeks interval for five times, and 18 weeks individuals were selected after 40 days of experimental time. External parameters of the eggs (egg weight, egg length, egg breadth and egg shape index, shell thickness), and body parameters (Final body weight, weight gain), feed intake and hen day production were measured. For egg length, egg breadth and shell thickness showed significant difference (p< 0.05) in from the control birds. Final body weight, weight gain, feed intake and hen day production showed comparable results with values from eggs of birds on control diet. The study revealed the ability of birds to easily utilize the nutrients in the protein sources. MSFLM utilization up to 15% in pullets' diet revealed no detrimental effect on the performance of the laying birds and the external qualities of eggs produced.

O uso de fontes nutricionais alternativas é um tópico importante para a produção animal, como a avicultura. Este estudo examinou o efeito da substituição do farelo de soja por farelo de folha de girassol na dieta de frangas marrons barbeadoras poedeiras. Um total de 30 aves poedeiras foram designadas a três tratamentos de 0% (como grupo controle), 10% e 15% de inclusão MSFLM. Quatro ovos por repetição foram retirados aleatoriamente por três dias consecutivos em intervalos de duas semanas por cinco vezes, e indivíduos de 18 semanas foram selecionados após 40 dias do tempo experimental. Parâmetros externos dos ovos (peso do ovo, comprimento do ovo, largura do ovo e índice de forma do ovo, espessura da casca) e parâmetros corporais (peso corporal final, ganho de peso), consumo de ração e produção diária da galinha foram medidos. Para comprimento do ovo, largura do ovo e espessura da casca apresentaram diferença significativa (p <0,05) nas aves controle. Peso corporal final, ganho de peso, consumo de ração e produção de dia de galinha mostraram resultados comparáveis ​​com valores de ovos de aves em dieta controle. O estudo revelou a capacidade das aves de utilizar facilmente os nutrientes das fontes de proteína. A utilização de MSFLM até 15% na dieta das frangas não revelou nenhum efeito prejudicial no desempenho das aves poedeiras e nas qualidades externas dos ovos produzidos.

Tendencia del programa de intervención en DemoMinga, como estrategia para mejorar las dislipidemias y glucemia en la población Norma Luisa, Minga Guazú

Antecedentes: Las enfermedades no transmisibles siguen siendo un problema creciente en el mundo, sobre todo en los países de mediano y bajo ingresos. Los programas de intervención comunitaria se enfocan en su disminución mediante cambios en estilos de vida más saludables. Objetivos: Analizar la tendencia del programa de intervención en actividad física y nutrición, como estrategia para mejorar las dislipidemias y glucemia en los participantes del proyecto DemoMinga. Materiales y métodos: Estudio con enfoque cuantitativo. Diseño de investigación acción participativa, de carácter longitudinal, prospectivo. La población estuvo constituida por los participantes del Proyecto DemoMinga. Se determinó en cada participante: colesterol total, triglicéridos y glucemia en ayunas. Las muestras fueron procesadas en el Centro de Investigaciones Médicas de la FACISA-UNE. Se determinaron indicadores de tendencia central (mediana) de cada una de las variables. Resultados: Hubo mayor participación del sexo femenino, con predominio de personas con menos de 45 años. Las mediciones químicas arrojaron una tendencia de disminución a partir del quinto año de intervención, con talleres de cocina saludable incluyendo uso de aceite alto oleico, y actividad física. Sin embargo, la prueba no arrojó diferencias significativas de las mediciones entre la línea de base y el corte a los 6 años. Conclusión: El estudio resalta la importancia de los programas de intervención mediante terapias integrales para la promoción y prevención de las enfermedades cardiometabólicas a largo plazo.

Background: Non-communicable diseases remain a growing problem worldwide, especially in middle and low-income countries. Community intervention programs are focused on reducing their prevalence through promoting healthier lifestyle changes. Objectives: To analyze the trend of the physical activity and nutrition intervention program as a strategy to improve dyslipidemia and glycemia among participants of the DemoMinga project. Materials and methods: This study employed a quantitative approach with a participatory action research design, characterized as longitudinal and prospective. The population consisted of participants from the DemoMinga Project. For each participant, total cholesterol, triglycerides, and fasting glycemia were measured. Samples were processed at the Medical Research Center of FACISA-UNE. Indicators of central tendency (median) were determined for each of the variables. Results: There was a higher participation of females, with a predominance of individuals under the age of 45. Chemical measurements showed a decreasing trend starting from the fifth year of intervention, involving healthy cooking workshops that included the use of high oleic oil and physical activity. However, the test did not yield significant differences in measurements between the baseline and the 6-year cutoff. Conclusion: The study highlights the significance of intervention programs using comprehensive therapies for the long-term promotion and prevention of cardiometabolic diseases.

Effect of fried sunflower oil intake on mandibular biomechanical competence of growing rats / Efecto del consumo de aceite de girasol termooxidado sobre la competencia biomecánica de la mandíbula, en animales en crecimiento

ABSTRACT Previous studies by us demonstrated that the consumption of thermally oxidized oil diet adversely affects body growth, lipid metabolism, bone mass and femur biomechanical competence. Aim: The aim of this study was to evaluate the effects of a diet containing fried sunflower oil on the mandible of growing rats. Materials and Method: Male Wistar rats (21±1 day old) (n=21) were assigned at weaning to one of three diets for 8 weeks: a control diet (C), a diet containing sunflower oil (SFO) or a diet containing sunflower oil that had been repeatedly heated (SFOx); both SFO and SFOx were mixed with commercial rat chow at 13% (w/w). The consistency and viscosity of the 3 diets were similar. Zoometrics and food intake were recorded weekly. At wk=8, mandibular growth was assessed by measurements of anatomical points of cleaned bones, and mandible biomechanical competence was assessed to estimate the structural properties of the bone. Statistical analysis was performed by SPSS v. 20.0. Results: Rats fed SFOx diet attained the lowest final body weight (P=0.0074), mandibular weight (P=0.0001) and mandibular /length (P=0.0002). Load bearing capacity (Wf;N), load of yielding (Wy;N) and stiffness (Wy/dy;N/mm) of the mandible were negatively affected by both sunflower oil diets (fresh and fried) (P=0.001; P=0.002; P=0.003, respectively) though SFOx induced the most significant reduction in Wy/dy (C:44.4(5.4) > SFO:36.1(2.1) > SFOx: 26.3(3.7) N/ mm; P=0.003). The deleterious effect of SFOx on mandibular growth was more accentuated on the posterior part of the bone (C:11.4(0.3)=SFO:11.2(0.2)>SFOx: 10.7(0.2) mm; p=0.0005); the anterior/ posterior ratio (C:1.25(0.02)=SFO:1.27(0.02)<SFOx:1.32(0.03); p=0.0001) indicated that SFOx induced mandibular deformation. Conclusion: Consumption of SFOx diet during growth could affect mandibular morphometric properties and biomechanical competence, in terms of bone stiffness.

RESUMEN En estudios previos hemos demostrado los efectos adversos del consumo de una dieta rica en aceite termooxidado sobre el crecimiento corporal, el metabolismo de los lípidos, la masa ósea y la competencia biomecánica del fémur. Objetivo: El objetivo de este trabajo fue investigar el efecto de una dieta rica en aceite de girasol termooxidado (AGX) sobre los parámetros morfométricos y biomecánicos de la mandíbula de rata en crecimiento. Materiales y Método: Ratas macho Wistar de 22±1 días de edad (n=21) recibieron durante 8 semanas una de 3 dietas: control (C); dieta comercial, una dieta suplementada con aceite de girasol (AG) y una dieta suplementada con AGX. La consistencia y la viscosidad de las dietas fueron similares. Los parámetros zoométricos y el consumo de dieta se registraron semanalmente. A T=8, los animales se eutanasiaron y se obtuvieron las hemimandíbulas. El crecimiento mandibular se estimó por medidas morfométricas entre puntos anatómicos y las propiedades estructurales por biomecánica. El análisis estadístico se realizó por SPSS v. 20.0. Resultados: Las ratas alimentadas con AGX presentaron menor peso corporal final (p=0.0074), peso mandibular (p=0.0001) y longitud mandibular (p=0.0002). Las propiedades estructurales de la mandíbula, Wf (p=0.001), Wy (p=0.002) y Wy/dy (p=0.003), se vieron afectadas negativamente en ratas alimentadas con AG o AGX, respecto a C; pero la rigidez ósea (Wy/dy) en AGX fue significativamente menor (C:44.4(5.4) > SFO:36.1(2.1) > SFOx: 26.3(3.7) N/mm; p=0.003). El efecto deletéreo del AGX sobre el crecimiento mandibular fue más acentuado en la región posterior (C:11.4(0.3)=SFO:11.2(0.2)>SFOx: 10.7(0.2) mm; p=0.0005). La relación anterior/posterior (C=1.25 (0.02); AG= 1.27(0.02) y AGX=1.32(0.03), p=0.001) indica que AGX indujo deformación mandibular. Conclusiones: El efecto adverso del consumo de una dieta rica en AGX durante el crecimiento podría afectar los parámetros morfométricos y la biomecánica ósea en términos de rigidez ósea.

Tendencia del programa de intervención en DemoMinga, como estrategia para mejorar las dislipidemias y glucemia en la población Norma Luisa, Minga Guazú / Trend of the DemoMinga intervention program as a strategy to improve dyslipidemia and glycemia in the Norma Luisa population, Minga Guazú

Antecedentes: Las enfermedades no transmisibles siguen siendo un problema creciente en el mundo, sobre todo en los países de mediano y bajo ingresos. Los programas de intervención comunitaria se enfocan en su disminución mediante cambios en estilos de vida más saludables. Objetivos: Analizar la tendencia del programa de intervención en actividad física y nutrición, como estrategia para mejorar las dislipidemias y glucemia en los participantes del proyecto DemoMinga. Materiales y métodos: Estudio con enfoque cuantitativo. Diseño de investigación acción participativa, de carácter longitudinal, prospectivo. La población estuvo constituida por los participantes del Proyecto DemoMinga. Se determinó en cada participante: colesterol total, triglicéridos y glucemia en ayunas. Las muestras fueron procesadas en el Centro de Investigaciones Médicas de la FACISA-UNE. Se determinaron indicadores de tendencia central (mediana) de cada una de las variables. Resultados: Hubo mayor participación del sexo femenino, con predominio de personas con menos de 45 años. Las mediciones químicas arrojaron una tendencia de disminución a partir del quinto año de intervención, con talleres de cocina saludable incluyendo uso de aceite alto oleico, y actividad física. Sin embargo, la prueba no arrojó diferencias significativas de las mediciones entre la línea de base y el corte a los 6 años. Conclusión: El estudio resalta la importancia de los programas de intervención mediante terapias integrales para la promoción y prevención de las enfermedades cardiometabólicas a largo plazo.

Background: Non-communicable diseases remain a growing problem worldwide, especially in middle and low-income countries. Community intervention programs are focused on reducing their prevalence through promoting healthier lifestyle changes. Objectives: To analyze the trend of the physical activity and nutrition intervention program as a strategy to improve dyslipidemia and glycemia among participants of the DemoMinga project. Materials and methods: This study employed a quantitative approach with a participatory action research design, characterized as longitudinal and prospective. The population consisted of participants from the DemoMinga Project. For each participant, total cholesterol, triglycerides, and fasting glycemia were measured. Samples were processed at the Medical Research Center of FACISA-UNE. Indicators of central tendency (median) were determined for each of the variables. Results: There was a higher participation of females, with a predominance of individuals under the age of 45. Chemical measurements showed a decreasing trend starting from the fifth year of intervention, involving healthy cooking workshops that included the use of high oleic oil and physical activity. However, the test did not yield significant differences in measurements between the baseline and the 6-year cutoff. Conclusion: The study highlights the significance of intervention programs using comprehensive therapies for the long-term promotion and prevention of cardiometabolic diseases.

New bicompartmental model: an application for the production of gases using the in vitro technique / Novo modelo bicompartimental: uma aplicação para a produção de gases pela técnica in vitro

The purpose of this study was to propose a bicompartmental nonlinear model and to identify the best-performing model between the proposed model and the bicompartmental logistic (BL) mode regarding the quality of fit to the curve of cumulative gas production (CGP) using corn silage, sunflower, and their mixtures. Gas production was measured 2, 3, 4, 6, 8, 9, 10, 12, 15, 19, 24, 30, 36, 48, 72, and 96 h after beginning the in vitro fermentation process. The generated data were used to generate the parameters of each model tested using the stats package of the R computational tool version 4.0.4. The mathematical models were subjected to the following selection criteria: the adjusted coefficient of determination (Raj.), residual mean square (RMS), mean absolute deviation (MAD), and Akaike information criterion (AIC). It was demonstrated that the proposed model had better performance with a high Raj., and lower values of RMS, AIC, and MAD than the bicompartmental logistic model for the prediction of the parameters of cumulative gas production (CGP), per to present a superior fit in the set of criteria according to the methodology and conditions in which the present study was developed.(AU)

No presente trabalho, com silagem de milho, girassol e suas misturas, objetivou-se propor um modelo não linear bicompartimental e identificar entre o modelo proposto e Logístico Bicompartimental (LB), aquele que apresenta maior qualidade de ajuste à curva de cinética de produção cumulativa de gases (PCG). A leitura da produção de gás foi realizada nos tempos 2, 3, 4, 6, 8, 9, 10, 12, 15, 19, 24, 30, 36, 48, 72 e 96 horas, após o início do processo de fermentação in vitro. Os dados gerados foram utilizados para geração dos parâmetros de cada modelo testado com auxílio do pacote stats da ferramenta computacional R versão 4.0.4. Os modelos matemáticos foram submetidos aos seguintes critérios de seleção o coeficiente de determinação ajustado (Raj.), quadrado médio do resíduo (QMR), desvio médio absoluto (DMA) e o critério de informação de Akaike (AIC). Foi demonstrado que o modelo proposto teve melhor desempenho com altos Raj., e menores valores de QMR, AIC e DMA, por apresentar um ajustamento superior no conjunto dos critérios em comparação com o modelo logístico bicompartimental para a predição dos parâmetros de produção cumulativa de gases (PCG) de acordo com a metodologia e condições em que foi desenvolvido o presente estudo.(AU)

Biopotency and toxicity of diverse group of insecticides under laboratory condition against sunflower defoliator Spilarctia obliqua (Walker)

The biopotency and toxicity of two groups, conventional and newer insecticides [eight commercial insecticides] were determined against Bihar or Common hairy caterpillar (BHC) Spilarctia obliqua (Walker). Experiment carried out in the laboratory by exposing BHC larvae to insecticides. Biopotency was measured at different time intervals from 12-72 h after insecticidal application showed that all of the insecticides outperformed the control. At 48 h after imposing, the highest mortality percentage (100) was observed on spinetoram 11.7 SC and chlorfenapyr 10 SC, which are comparable to cyantranaliprole 10.26 OD, followed by chlorantraniliprole 18.5 SC, ?-cyhalothrin 5 EC and chlorpyriphos 20 EC, with the lowest mortality percentage observed on tolfenpyrad 15 EC (66.67 %). In chlorantraniliprole, cyantranaliprole, spinetoram, tolfenpyrad, fipronil, chlorfenapyr, ?-cyhalothrin and chlorpyriphos, respectively, the toxicity level of insecticides was calculated based on median lethal concentration (LC50) values of 0.36, 0.48, 0.52, 0.50, 0.69, 0.98, 1.43 and 3.82 ppm. The overall conclusion of this trial was that spinetoram 11.7 SC and chlorfenapyr10 SC are the best chemicals for controlling the early stages of BHC larvae. In comparison to traditional insecticides, new insecticides have the highest level of toxicity

Biosynthesis of lipase by Burkholderia cenocepacia ST8 using waste cooking oil as feedstock

Aims@#Every year, an estimated 25 million tons of waste oil are produced worldwide, and the generation of waste oil is one of the biggest global environmental problems. The incorporation of oil as a substrate for lipase production has been studied and shown to have a positive impact on its production. Burkholderia sp. is one of the major lipase-producing bacteria with their ability in bioremediation of oil-contaminated soil. This study aims to compare the production of lipase by Burkholderia cenocepacia ST8 using waste cooking oil and unused cooking oil as feedstock.@*Methodology and results@#The effect of different types of waste cooking oil (sunflower oil and palm oil) and concentration (1-3%) of waste cooking oil, agitation speed (100-400 rpm) and initial dissolved oxygen concentration (10-50%) on lipase production by B. cenocepacia ST8 under batch fermentation mode were investigated. The major fatty acids of which had been consumed were determined using gas chromatography. Results showed that 2% (v/v) of single used sunflower cooking oil produced the highest lipase activity of 138.86 U/mL with a productivity of 2.10 U/mL/h; agitation speed of 300 rpm produced the highest lipase activity of 183.56 U/mL with a productivity of 3.06 U/mL/h while 30% initial concentration of dissolved oxygen produced a lipase activity of 176.45 U/mL with a productivity of 2.94 U/mL/h. Oleic acid and linoleic acid were found to be the most consumed by B. cenocepacia ST8 among other fatty acids. @*Conclusion, significance and impact of study@#This study shows that 2% (v/v) single used sunflower cooking oil was the better type and optimum concentration of carbon source for the production of lipase by the fermentation of B. cenocepacia under 300 rpm and 30% initial concentration dissolved oxygen. The incorporation of 2% (v/v) single used sunflower cooking oil may be a great alternative to reduce the cost for the production of lipase as well as reducing the amount of waste oil generation.

Cytomorphological and molecular characterization of inter-specific hybrid between cultivated sunflower and Helianthus argophyllus

Aim: To study useful variability generated in an inter-specific hybrid between cultivated sunflower inbred (ARM-243B) and silver leaf sunflower, H. argophyllus (ARG-2126) through cytomorphological traits and hybrid confirmation by molecular markers.Methodology: The present study on hybridization was undertaken using cultivated sunflower inbred (ARM-243B) and silver leaf sunflower H. argophyllus (ARG-2126). Thirty one morphological characters were recorded of cultivated sunflower (ARM-243B), H. argophyllus (ARG-2126) and inter-specific hybrid. A total of 50 PMCs were analysed for cytological observations. 62 SSR primers were used for molecular characterisation and hybridity confirmation in inter-specific hybrid. Results: Considerable variability was observed in inter-specific hybrid, with common traits including branched stems, anthocyanin coloration in petiole and disk florets, leaf petioles, and stems etc. Cytological abnormalities and reduction in pollen fertility indicated that generated inter-specific hybrid was true type. Out of sixty two SSR primers, nineteen showed parental polymorphism. ORS-1021 showed 10 bp allelic variation in inter-specific hybrid in a co-dominant manner and, hence, was used for hybrid confirmation. Interpretation: The results of this investigation showed that inter-specific hybridization between cultivated sunflower and H. argophyllus and transfer of desirable traits into cultivated sunflower is possible.

A Comparative Study of the Effect of Flaxseed Oil and Sunflower Oil on the Coagulation Score, Selected Oxidative and Inflammatory Parameters in Metabolic Syndrome Patients

0.05). However, serum IL-6 levels significantly decreased in the flaxseed oil group compared to the sunflower oil group (p = 0.017). No side effect was observed during the study due to the use of sunflower and flaxseed oils. We observed that consumption of flaxseed oil improved serum IL-6 levels but had no effect on oxidative stress and coagulation score in patients with MetS. Further studies are needed to confirm the veracity of our results.TRIAL REGISTRATION: Iranian Registry of Clinical Trials Identifier: IRCT2015012020737N1

Sunflower meal with and without phytase supplementation in diets for silver catfish (Rhamdia quelen) juveniles / Harina de girasol con y sin suplementación de fitasa en dietas para juveniles de bagre (barbudo, Col.; Rhamdia quelen) / Farelo de girassol com e sem suplementação de fitase em dietas para juvenis de jundiá ( Rhamdia quelen)

Abstract Background: The cost of feed in fish farming can exceed 70% of the total costs. Thus, the feed industry have sought to formulate balanced diets including low-cost by-products. Objective: To evaluate sunflower meal as a partial substitute (0, 10, and 20%) for soybean meal, with or without phytase supplementation (1,500 FTU/kg) in isoproteic (27% crude protein (CP)) and isoenergetic diets (3,400 kcal/ED/kg of feed) for silver catfish (Rhamdia quelen) juveniles. Methods: A total of 360 fish (weight: 15.77 ± 0.56 g) were randomly distributed in 24 tanks in a completely randomized design following a factorial scheme (3x2) with 4 replicates. The fish were evaluated on the following variables after 90 days: productive performance, hematological variables, histology of intestinal tissue, bromatological composition of carcass, and composition of bone mineral matter. Results: 20% dietary inclusion of sunflower meal improved weight gain and protein efficiency. No difference was observed in the other treatments and no interactions were observed between the other factors. Conclusion: 20% sunflower meal can be included in the feed of silver catfish as a substitute for soybean meal. Phytase inclusion (1,500 FTU/kg) did not improve the nutritional value of the treatments.

Resumen Antecedentes: El costo de la alimentación en piscicultura pueden superar el 70% del costo total. Las industrias de alimentos para peces han intentado formular dietas balanceadas con subproductos de menor valor en el mercado para minimizar costos. Objetivo: Evaluar la harina de girasol como sucedáneo parcial (0, 10 y 20%) de la torta de soya, suplementada o no con fitasa (1.500 FTU/kg) en dietas isoprotéicas (27% de PB) e isoenergéticas 3400 kcal / ED / kg de ración) para bagre (barbudo, Col.; Rhamdia quelen). Métodos: Los peces, 360 juveniles (15,77 ± 0,56 g), se distribuyeron aleatoriamente en 24 unidades experimentales, en un diseño completamente al azar, en esquema factorial (3x2) y 4 repeticiones. A los 90 días se evaluó: desempeño productivo, variables hematológicas, histología de tejido intestinal y composición bromatológica de la carcasa y de la materia mineral de los huesos. Resultados: La inclusion de 20% de harina de girasol mejoró la ganancia de peso de los peces y la tasa de eficiencia proteica de la ración, sin diferencia para las otras evaluaciones ni interacción entre los demás factores evaluados. Conclusión: La harina de girasol se puede utilizar en un 20% de la ración del bagre (barbudo, Col.) como sucedáneo a la harina de soja. La inclusión de fitasa (1.500 FTU/kg) no mejoró el valor nutritivo de estas raciones.

Resumo Antecedentes: Os custos relativos a alimentação na piscicultura pode ultrapassar 70% do custo final, neste sentido, as indústrias de rações têm procurado formular dietas balanceadas com subprodutos de menor valor de mercado para minimizar estes custos. Objetivo: Esse estudo objetivou avaliar o farelo de girassol como sucedâneo parcial (0, 10 e 20%) do farelo de soja, suplementado ou não com a fitase (1.500 FTU/kg), em dietas isoprotéicas (27% de PB) e isoenergéticas (3400 kcal/ED/kg de ração) para o jundiá (Rhamdia quelen). Métodos: Os peixes, 360 juvenis (15,77 ± 0,56 g) foram distribuídos aleatoriamente em 24 unidades experimentais, num delineamento inteiramente casualizado, em esquema fatorial (3x2) e 4 repetições. Após 90 dias foram avaliados: desempenho produtivo, variáveis hematológicas, histologia de tecido intestinal, composição bromatológica da carcaça e de matéria mineral dos ossos. Resultados: O emprego de 20% de farelo de girassol melhorou o ganho de peso dos peixes e a taxa de eficiência protéica da ração, sem diferença nas outras avaliações, ou para a interação entre os demais fatores avaliados. Conclusão: Pode-se concluir que o farelo de girassol pode ser utilizado em 20% da ração do jundiá, como sucedâneo ao farelo de soja e, que a inclusão de fitase (1,500 FTU/kg) não melhorou o valor nutritivo destas rações.

The Beneficial Effect Of 20% Sunflower Seed Oil Cream On Mild Atopic Dermatitis In Children

Objective: The objective of this study was to evaluate the beneficial effect of topical applications of 20% sunflower seed oil (SSO) in decreasing the transepidermal water loss (TEWL) and scoring of atopic dermatitis (SCORAD) index value in mild atopic dermatitis (AD) pediatric patients in Bandung, Indonesia. Methods: This was a randomized study involving 20 children with mild AD (SCORAD<25) in Bandung, Indonesia. They were divided into 2 groups: the experimental group treated with 20% SSO cream and the control group treated with a common moisturizing cream for four weeks. The TEWL score including SCORAD index was evaluated at baseline, week 1, week 2, and week 4. Results: A total of 20 participants completed the study. In the first week, the control group had TEWL score decrement by 36.62% while the experimental group by 28.89% (p=0.88). In the fourth week, the TEWL decrements of the experimental and control group were by 56.94% and 52.50%, respectively (p=0.20), and this was followed by an improvement of SCORAD index in both treatment groups. Conclusion: The 20% SSO cream has a beneficial effect in decreasing the TEWL score and improving SCORAD indices. Therefore, it can be considered as an alternative treatment for mild AD among children.

Desenvolvimento de oleogéis à base de óleo de girassol alto oleico estruturados por monoestearato de sorbitana e cera de candelilla / Development of high oleic sunflower oil oleogel structured with candelilla wax and sorbitan monostearate

As gorduras ricas em ácidos graxos saturados e/ou trans são ingredientes que brindam propriedades nos alimentos como textura, fusão e estabilidade. Porém, a relação entre seu consumo e enfermidades cardiovasculares, força a busca de novas alternativas. Uma alternativa potencial são os oleogéis, pois são um material plástico e com melhor balanço lipídico, do ponto de vista nutricional. Os oleogéis são sistemas coloidais onde uma fase orgânica líquida, representada por um óleo, é imobilizada por uma rede sólida tridimensional formada por agentes estruturantes. Atualmente, tem sido estudados oleogéis conformados com variados agentes estruturantes, com o intuito de encontrar a melhor opção. Além disso, alguns estudos indicam que o uso combinado de dois ou mais agentes estruturantes pode resultar no aumento de seu potencial de estruturação de óleos ou na possibilidade de customizar as propriedades adequadas para produção alimentar. Assim, o objetivo deste estudo é avaliar a interação entre os componentes de oleogéis preparados com óleo de girassol alto oleico (HOSO), como base lipídica, e combinações de cera de candelilla (CW) e monoestearato de sorbitana (SMS), como agentes estruturantes. A avaliação dos oleogéis por meio dos diagramas de fases e a consistência, mostrou que há uma sinergia entre os componentes, na concentração de 8 e 4% de agentes estruturantes e 79-89% de cera de candelilla. Estes oleogéis mistos tiveram uma faixa de fusão maior do que os oleogéis não mistos. Sugerindo a construição de cristais mais estáveis, que demoraram mais tempo em fundir. A capacidade de retenção de óleo se mostrou adequada (99%) nas amostras contendo mais de 1% de agentes estruturantes e com mais de 40% de Cera de Candelilla. Nas imagens obtidas por PLM, foi observada uma variedade de tamanho de cristais, sugerindo que cada componente do oleogel formou um tipo de cristal. Com a elaboração de bigéis, foi notado que, para obter um produto satisfatoriamente espalhável ou para observar o aumento de sua consistência em comparação com o oleogel isoladamente, é necessária uma concentração maior do que 4% de agentes estruturantes no oleogel e/ou a adição de menos de 20% de água na formulação total. A estabilidade dos bigéis foi beneficiada pela goma xantana e pela mistura de 2:1 de goma xantana e goma guar na concentração de 0,3%. Dessa forma, o desenvolvimento de alimentos com teores reduzidos de gorduras saturadas e ao mesmo tempo livres de gorduras trans se faz possível com a formulação de oleogéis, o que poderá contribuir para melhorar a qualidade nutricional dos alimentos industrializados oferecidos à população

Fats rich in saturated and / or trans fatty acids provide properties in food such as texture, fusion and stability. But, since those fats increase cardiovascular disease risk, alternatives have been searched. A potential alternative is oleogels, as they are a plastic material with better lipid balance from a nutritional point of view. Oleogels are colloidal systems where a liquid organic phase, represented by an oil, is immobilized by a three-dimensional solid network formed by structuring agents. Currently, oleogels conformed with various structuring agents have been studied in order to find the best option. In addition, some studies indicate that the combined use of two or more structuring agents may result in increased oil structuring potential or the possibility of customizing the properties suitable for food production. Thus, the aim of this study is to evaluate the interaction between the components of oleogels prepared with high oleic sunflower oil (HOSO) as a lipid base and combinations of candelilla wax (CW) and sorbitan monostearate (SMS) as structuring agents. The evaluation of oleogels by phase diagrams and consistency (Yield value) showed that there is a synergy between the components, in the concentration of 8 and 4% of structuring agents and 79-89% of candelilla wax. These mixed oleogels had a higher melting range than unmixed oleogels. Suggesting the construction of more stable crystals, which took longer to fuse. Oil binding capacity was adequate (99%) in samples containing more than 1% of structuring agents and over 40% of candelilla wax. In the PLM images, a variety of crystal sizes were observed, suggesting that each oleogel component formed a crystal type. With the elaboration of bigels, it was noted that in order to obtain a satisfactorily spreadable product or to observe the increase of its consistency compared to the oleogel alone, a concentration greater than 4% of structuring agents in the oleogel and / or the addition of less than 20% water in the total formulation. The stability of the bigels was benefited by xanthan gum and the 2: 1 mixture of xanthan gum and guar gum at a concentration of 0.3%.Thus, the development of foods with reduced levels of saturated fat and at the same time free of trans fats is possible with the formulation of oleogels, which may contribute to improve the nutritional quality of processed foods offered to the population

Therapeutic effect of sunflower powder active ingredients on hyperuricemia in mice / 吉林大学学报(医学版)

Objetive: To investigate the therapeutic effects of small peptide, alkaloid, total flavonoids and polysaccharides extracted from sunflower plate powder on the hyperuricemia in the mice, and to clarify that the influence of sunflower powder in the uric acid (UA) level and joint swelling and its protective effect on the liver and kidney tissues. Methods: A total of 96 male Kunming rats were randomly divided into 8 groups, each group of 12 rats. One group was used as blank control group, the others were used to set up hyperuricemia models. The successful mouse models of hyperuricemia were randomly divided into model group and positive control group (allopurinol group), small molecule peptide group, alkaloi group, total flavone group, polysaccharide group and sunflower disk group. The serum UA levels were measured 7 d after administration and the liver and kidney tissues of the mice in various groups were taken. The morphological changes of liver and kidney tissues were observed by HE staining. Acute gouty arthritis model was established by injecting 3 mg sodium urate into the knee joint of the male SD rats. The experiment was divided into blank group, model group, positive control colchicine group, sunflower disc extracts (SDE) group with different concentrations (0%, 20%, 40%, 60%, 80% and 100%), 4 rats in each group. The joint circumference was measured at 0, 12, 24 and 48 h, respectively. The serum levels of interleukin-10 (IL-10) and macrophage inflammatory protein-la (MIP-la) were detected by ELISA kit. Results: Compared with model group, the serum UA levels of the mice in allopurinol group, small molecule peptide group, alkaloid group, total flavonoids group and sunflower group were significantly decreased after administration (P<0.05); and the serum UA level in allopurinol group was decreased the most, followed by small molecule peptide group. The HE staining results showed that the hepatocytes in small molecule peptide group were clear, the hepatocytes had full cytoplasm, and no abnormality was observed in the kidney cells compared with blank control group. After 48 h injection of sodium urate, the degree of joint swelling was reduced by up to 27.1% in SDE group compared with model group. Compared with model group, the serum IL-10 level in 20% SDE group was significantly increased (P<0.01), and the serum MIP-la level was also significantly increased (P<0.01). Conclusion: Sunflower powder can reduce the UA level and has protective effect on the liver and kidney tissues and it can improve the body's anti-inflammatory activity and reduce the body's inflammatory response to achieve anti-gout arthritis activity.

Therapeutic effect of sunflower powder active ingredients on hyperuricemia in mice / 吉林大学学报(医学版)

Objective:To investigate the therapeutic effects of small peptide,alkaloid,total flavonoids and polysaccharides extracted from sunflower plate powder on the hyperuricemia in the mice,and to clarify that the influence of sunflower powder in the uric acid(UA)level and joint swelling and its protective effect on the liver and kidney tissues.Methods:A total of 96 male Kunming rats were randomly divided into 8 groups,each group of 12 rats.One group was used as blank control group,the others were used to set up hyperuricemia models.The successful mouse models of hyperuricemia were randomly divided into model group and positive control group (allopurinol group),small molecule peptide group,alkaloi group,total flavone group,polysaccharide group and sunflower disk group.The serum UA levels were measured 7 d after administration and the liver and kidney tissues of the mice in various groups were taken.The morphological changes of liver and kidney tissues were observed by HE staining.Acute gouty arthritis model was established by injecting 3 mg sodium urate into the knee joint of the male SD rats.The experiment was divided into blank group,model group,positive control colchicine group, sunflower disc extracts(SDE)group with different concentrations(0%,20%,40%,60%,80% and 100%), 4 rats in each group.The joint circumference was measured at 0,12,24 and 48 h,respectively.The serum levels of interleukin-10(IL-10)and macrophage inflammatory protein-1α(MIP-1α)were detected by ELISA kit. Results:Compared with model group,the serum UA levels of the mice in allopurinol group,small molecule peptide group,alkaloid group,total flavonoids group and sunflower group were significantly decreased after administration (P<0.05);and the serum UA level in allopurinol group was decreased the most,followed by small molecule peptide group.The HE staining results showed that the hepatocytes in small molecule peptide group were clear, the hepatocytes had full cytoplasm,and no abnormality was observed in the kidney cells compared with blank control group.After 48 h injection of sodium urate,the degree of joint swelling was reduced by up to 27.1% in SDE group compared with model group.Compared with model group,the serum IL-10 level in 20% SDE group was significantly increased(P<0.01),and the serum MIP-1α level was also significantly increased(P<0.01). Conclusion:Sunflower powder can reduce the UA level and has protective effect on the liver and kidney tissues and it can improve the body's anti-inflammatory activity and reduce the body's inflammatory response to achieve anti-gout arthritis activity.

Levels of antioxidant enzymes and alkaline protease from pulp and peel of sunflower

Objective The activity of enzymes participating in the systems of antioxidant protection was assayed in the peel and pulp of sunflower. The essential roles of proteases in food stimulate research to find other sources of the enzyme especially from non-conventional sources. In the present work, we study several biochemical parameters in the pulp and peel of sunflower. Methods Pulp and peel of sunflower was extracted, antioxidant enzymes and non-enzymatic antioxidant were measured. Alkaline protease was measured and purified from pulp in sunflower. Results High carbohydrate concentration, beta-carotene, catalase and ascorbate peroxidase activities, free radical scavenging capacity and free flavonoid content were observed in the peel of sunflower. Whereas, MDA and ceruloplasmin activities were high in the pulp of sunflower. Conclusions The present study concluded that peel in sunflower are strong radical scavengers and can be considered as good sources of natural antioxidants for medicinal and commercial uses. Further analysis showed that protease activity was a significantly high in the pulp compared to the peel.

Effect of dietary supplementation with olive and sunflower oils on lipid profile and liver histology in rats fed high cholesterol diet

Objective To compare the effects of high-monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) against the metabolic disorders elicited by a high-cholesterol diet (HC) in rats. Methods Using in vivo dietary manipulation, rats were fed with different diets containing 4% soybean oil (cholesterol free diet) and 1% HC containing 12% olive oil (HC + OO) enriched with MUFA and 12% sunflower oil (HC + SO) enriched with PUFA for 60 d. Serum lipid levels and hepatic steatosis were evaluated after the treatment period. Results Comparatively, rats treated with HC + OO diet experienced a decrease in the serum LDL-C, VLDL-C and CT levels compared to those fed with HC + SO diet (P < 0.05). Otherwise, HC + OO provoked significant microvesicular steatosis situated in the hepatic acinar zone 1. Conclusions HC + OO diet has high absorption velocity in the acinar zone 1 of liver compared to the HC + SO diet. Based on this, the reduction of the LDL-C, VLDL-C and CT serum levels in the animals treated with HC + OO diet may have been caused by the delay in the FA release to the blood.

Effect of dietary supplementation with olive and sunflower oils on lipid profile and liver histology in rats fed high cholesterol diet

OBJECTIVE@#To compare the effects of high-monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) against the metabolic disorders elicited by a high-cholesterol diet (HC) in rats.@*METHODS@#Using in vivo dietary manipulation, rats were fed with different diets containing 4% soybean oil (cholesterol free diet) and 1% HC containing 12% olive oil (HC + OO) enriched with MUFA and 12% sunflower oil (HC + SO) enriched with PUFA for 60 d. Serum lipid levels and hepatic steatosis were evaluated after the treatment period.@*RESULTS@#Comparatively, rats treated with HC + OO diet experienced a decrease in the serum LDL-C, VLDL-C and CT levels compared to those fed with HC + SO diet (P < 0.05). Otherwise, HC + OO provoked significant microvesicular steatosis situated in the hepatic acinar zone 1.@*CONCLUSIONS@#HC + OO diet has high absorption velocity in the acinar zone 1 of liver compared to the HC + SO diet. Based on this, the reduction of the LDL-C, VLDL-C and CT serum levels in the animals treated with HC + OO diet may have been caused by the delay in the FA release to the blood.

Conversão enzimática de triacilgliceróis em mono e diacilgliceróis de interesse industrial / Enzymatic conversion of triacylglycerols to mono and diacilglycerols of industrial interest

Mono e diacilgliceróis são produtos empregados na indústria alimentícia, farmacêutica, cosmética e química como emulsificantes e melhoradores de viscosidade de produtos alimentícios, cosméticos e farmacêuticos. No entanto, a forma mais usual de obtê-los é por síntese química, o que acaba rendendo produtos finais caros e com atributos de qualidade, rendimento e de aplicabilidade tecnológica inferiores aos esperados. A busca por formas de obtenção mais racionais, eficientes e com melhor padrão de qualidade destes produtos foi o objetivo principal do trabalho, por meio de hidrólise parcial enzimática, que necessita de condições de reação mais brandas. Foram avaliadas a hidrólise enzimática descontína, empregando como substrato a trioleína técnica, e a hidrólise enzimática descontínua-alimentada, usando como substrato o óleo de girassol médio oléico. Foi utilizada, em ambos processsos, a lipase imobilizada sn-1,3 específica Lipozyme RM IM (de Rhizomucor miehei). A caracterização dos padrões e dos substrados, bem como o acompanhamento da formação dos produtos da hidrólise enzimática foram feitos por determinação da porcentagem de hidrólise, cromatografia em camada delgada (TLC), dos perfis das curvas de fusão e cristalização por calorimetria diferencial de varredura (DSC), cromatografia gasosa (CG) e cromatografia de exclusão de tamanho de alto desempenho (HPSEC). Os parâmetros de hidrólise descontínua foram o tempo de reação, a temperatura e a concentração inicial de substrato. Os parâmetros de hidrólise descontínua-alimentada foram tempo de enchimento e intervalo de alimentação de substrato. Para as respostas analíticas de porcentagem de hidrólise e de composição de frações lipídicas foi aplicado um modelo de regressão múltipla com base em metodologia de superfície de resposta. Os resultados experimentais observados nas reações de hidrólise enzimática descontínua de trioleína técnica mostraram de 24,7 a 34,2% de mono e diacilgliceróis (para 5% de óleo na emulsão) e de 21,4 a 33,6% de mono e diacilgliceróis (para 20% de óleo na emulsão). Os resultados experimentais observados nas reações de hidrólise enzimática descontínua-alimentada de óleo de girassol médio oléico (para 15% de óleo na emulsão), mostraram de 7,9 a 31,8% de mono e diacilgliceróis. Os modelos de superfície de resposta foram considerados significativos e preditivos. As hidrólises obtidas no formato descontínuo e descontínuo-alimentado permitiram efetivamente a obtenção de frações de mono/ diacilgliceróis com vários graus de eficiência de conversão e com corretas identificação e quantificação das frações de lipídios procuradas. As correlações feitas entre porcentagem de hidrólise e entalpias de cristalização e fusão, corroboradas com os resultados qualitativos e/ou quantitativos diretos obtidos na cromatografia de camada delgada (TLC) e de HPSEC, demonstraram que estes atributos podem positivamente indicar a ocorrência efetiva de reação de hidrólise, além de auferir uma escala de desempenho de reação alinhada com o previsto na literatura, à medida que são aumentadas a temperatura, o tempo de hidrólise e a porcentagem inicial de substrato oleoso, sob regime descontínuo, e que puderam ser melhoradas, de forma inovadora, sob parâmetros de tempo total de alimentação e de intervalo de alimentação, sob regime descontínuo-alimentado. A hidrólise parcial enzimática de triacilgliceróis utilizando lipase imobilizada sn-1,3 específica pode ser considerada uma alternativa às vias químicas para a produção de misturas de mono e diacilgliceróis para utilização como aditivos químicos.

Mono and diacylglycerols are products used in the food, pharmaceutical, cosmetic and chemical industries as emulsifiers and viscosity improvers for food products, cosmetics and pharmaceuticals. However, the most usual forms of obtaining them are by chemical synthesis, which ends up yielding expensive final products with attributes of quality, yield and technological applicability lower than expected. The search for more rational, efficient and better quality standards of these products was the aim of the work, through partial enzymatic hydrolysis, which requires milder reaction conditions. Discontinuous enzymatic hydrolysis was evaluated using technical triolein as substrate and discontinuous-fed enzymatic hydrolysis using as the substrate the mid oleic sunflower oil. In both processes, immobilized lipase sn-1,3 specific Lipozyme RM IM (from Rhizomucor miehei) was used. The characterization of the patterns and substrates, as well as the monitoring of the formation of the products from the enzymatic hydrolysis were made by determining the percentage of hydrolysis, thin layer chromatography (TLC), profiles of the melting and crystallization curves by differential scanning calorimetry ( DSC), gas chromatography (GC) and high performance size exclusion chromatography (HPSEC). The parameters of discontinuous hydrolysis were the reaction time, the temperature and the initial substrate concentration. The parameters of discontinuous-fed hydrolysis were filling time and substrate feed interval. For the analytical responses of hydrolysis percentage and composition of lipid fractions a multiple regression model was applied based on response surface methodology. The experimental results observed in the reactions of discontinuous enzymatic hydrolysis of technical triolein indicated amounts of mono- and diacylglycerols from 24.7 to 34.2% (for 5% of oil in the emulsion) and from 21.4 to 33.6% for mono and diacylglycerols with 20% oil in the emulsion. The experimental results observed in the reactions of discontinuous-fed enzymatic hydrolysis of mid oleic sunflower oil (for 15% oil in the emulsion), showed from 7.9 to 31.8% of mono and diacylglycerols. Response surface models were considered significant and viii predictive. The hydrolysis obtained in the discontinuous and discontinuous-fed form allowed to obtain fractions of mono / diacylglycerols with various degrees of conversion efficiency and with correct identification and quantification of the lipid fractions sought. The correlations between the percentage of hydrolysis and enthalpies of crystallization and fusion, corroborated with the qualitative and / or quantitative direct results obtained in thin layer chromatography (TLC) and HPSEC, showed that these attributes can positively indicate the effective occurrence of reaction of Hydrolysis, in addition to achieving a reaction performance scale in line with the literature, as the temperature rate, the hydrolysis time and the initial percentage of oily substrate are increased under a discontinuous regime and can be improved, in a innovative form, under parameters of total filling time and feeding interval, under a fed-batch regime. The partial enzymatic hydrolysis of triacylglycerols using specific sn-1,3-specific immobilized lipase may be considered an alternative to the chemical pathways for the production of mono- and diacylglycerol blends for use as chemical additives.

Levels of antioxidant enzymes and alkaline protease from pulp and peel of sunflower

Objective: The activity of enzymes participating in the systems of antioxidant protection was assayed in the peel and pulp of sunflower. The essential roles of proteases in food stimulate research to find other sources of the enzyme especially from non-conventional sources. In the present work, we study several biochemical parameters in the pulp and peel of sunflower. Methods: Pulp and peel of sunflower was extracted, antioxidant enzymes and non-enzymatic antioxidant were measured. Alkaline protease was measured and purified from pulp in sunflower. Results: High carbohydrate concentration, beta-carotene, catalase and ascorbate perox-idase activities, free radical scavenging capacity and free flavonoid content were observed in the peel of sunflower. Whereas, MDA and ceruloplasmin activities were high in the pulp of sunflower. Conclusions: The present study concluded that peel in sunflower are strong radical scavengers and can be considered as good sources of natural antioxidants for medicinal and commercial uses. Further analysis showed that protease activity was a significantly high in the pulp compared to the peel.